Fungicide pyraclostrobin has been widely employed to control plant diseases by inhibiting the mitochondrial respiration of pathogenic fungi. Due to its broad spectrum, the extensive use of pyraclorstrobin was reported to cause emerging resistance on crops. Here, we evaluated the control effect of 250 g L-1 of pyrachlostrobin suspension concentrate (SC) against freckle disease (caused by Phyllosticta spp) on banana. Meanwhile, the dissipation and residue dynamics of pyraclostrobin in banana and soil under field conditions were determined by high performance liquid chromatography (HPLC) with DAD detection in different locations. The analytical method was validated using spiked samples at three levels, which indicated the recoveries ranged from 92.0% to 99.0% with relative standard deviations (RSDs) below 5%, providing a sensitive, precise and reliable method to monitor pyraclostrobin in banana fruit and soil. The dissipation of pyraclostrobine followed the first-order kinetics and its half-lives were 5.25 to 9.90 days. In addition, the terminal residues of pyraclostrobin in banana, banana sarcocarp and soil were below the maximum residue limit (MRL) (0.02 mg kg-1) after a pre-harvest interval (PHI) of 42 days, which suggesting that the use of pyraclostrobin at recommended dosages was safe to banana and the environment. In summary, we demonstrated the integrated evaluation on the disease control capacity of pyraclostrobin and its environmental behavior on banana, aiming to provide solid and basic data for the safe use of fungicide pyraclostrobin.