Ammonium is a paradoxical chemical because it is a nutrient but also damages ecosystems at high concentration. As the most eco-friendly method of water restoration, phytoremediation technology still faces great challenges. To provide more theoretical support, we exploited six common submerged macrophytes and selected the most ammonium-tolerant and -sensitive species; then further explored and compared the mechanisms underlying ammonium detoxification. Our results showed the activity of glutamate dehydrogenase (GDH) in the ammonium-tolerant species Myriophyllum spicatum leaves performed a dose-response curve (increased 169% for NADH-dependent GDH and 103% for NADPH-dependent GDH) with the [NH₄⁺-N] increasing from 0 to 100 mg/L while glutamine synthetase (GS) activity slightly changed. But for the ammonium-sensitive species, Potamogeton lucens, the activity of GDH recorded no major changes, while the GS increased slightly (17%). Based on this, we conclude that the alternative pathway of GDH is more important than the pathway catalyzed by GS in determining the tolerance of submerged macrophytes to high ammonium concentration (up to 100 mg N/L). Our present study identifies submerged macrophytes that are tolerant of high concentrations of ammonium and provides mechanistic support for practical water restoration by aquatic plants.