Abstract:

The stimulation by KCl of stomatal opening in isolated epidermal strips of Vicia faba was examined. In dark + normal air the opening response was maximal at 100 mm KCl while in light + CO(2)-free air it was maximal at about 10 mm KCl. CO(2)-free air was more influential than light in reducing the KCl concentration required for maximal opening. K(+) was essential while Cl(-) seemed to be of secondary importance in these processes.The use of (86)Rb(+) as a tracer for K(+) showed that the increase in stomatal aperture under various conditions was well correlated with K(+) uptake. The estimated amount of K(+) taken up by guard cells, along with a counter ion, was sufficient to account for the changes in solute potential associated with opening. It is suggested that the absorption of extracellular solutes, such as K(+), may be the primary mechanism of stomatal opening. Both opening and K(+) absorption are stimulated by light + CO(2)-free air.The increase in stomatal aperture was also well correlated with the decrease in stainable starch in guard cells under all conditions. It is suggested that this is a secondary change, although perhaps closely linked to K(+) absorption.

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Stomatal Opening in Isolated Epidermal Strips of <em>Vicia faba</em>. II. Responses to KCl Concentration and the Role of Potassium Absorption <sup><a href="#fn1" rid="fn1" class=" fn">1</a></sup>

R Fischer

T Hsiao

Abstract

The stimulation by KCl of stomatal opening in isolated epidermal strips of Vicia faba was examined. In dark + normal air the opening response was maximal at 100 mm KCl while in light + CO₂-free air it was maximal at about 10 mm KCl. CO₂-free air was more influential than light in reducing the KCl concentration required for maximal opening. K^{was essential while Cl}^{seemed to be of secondary importance in these processes.}

The use of ^Rb^{as a tracer for K}^{showed that the increase in stomatal aperture under various conditions was well correlated with K}^{uptake. The estimated amount of K}^{taken up by guard cells, along with a counter ion, was sufficient to account for the changes in solute potential associated with opening. It is suggested that the absorption of extracellular solutes, such as K}^{, may be the primary mechanism of stomatal opening. Both opening and K}^{absorption are stimulated by light + CO}₂-free air.

The increase in stomatal aperture was also well correlated with the decrease in stainable starch in guard cells under all conditions. It is suggested that this is a secondary change, although perhaps closely linked to K^absorption.

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Fischer RA. Stomatal opening: role of potassium uptake by guard cells. Science. 1968 May 17;160(3829):784–785. [PubMed] [Google Scholar]
Fischer RA. Stomatal Opening in Isolated Epidermal Strips of Vicia faba. I. Response to Light and to CO(2)-free Air. Plant Physiol. 1968 Dec;43(12):1947–1952.[PMC free article] [PubMed] [Google Scholar]
Hiatt AJ. Relationship of Cell Sap pH to Organic Acid Change During Ion Uptake. Plant Physiol. 1967 Feb;42(2):294–298.[PMC free article] [PubMed] [Google Scholar]
Rains DW. Light-enhanced potassium absorption by corn leaf tissue. Science. 1967 Jun 9;156(3780):1382–1383. [PubMed] [Google Scholar]
Rains DW. Kinetics and Energetics of Light-enhanced Potassium Absorption by Corn Leaf Tissue. Plant Physiol. 1968 Mar;43(3):394–400.[PMC free article] [PubMed] [Google Scholar]

Laboratory of Plant-Water Relations, Department of Water Science and Engineering, University of California, Davis, California 95616

^{Present address: Research School of Biological Sciences, Australian National University, Canberra, A.C.T., Australia.}

^{Supported in part by grant B-029-CAL from the Office of Water Resources Research, United States Department of the Interior, and by a grant from the Water Resources Center, University of California.}

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Abstract

The stimulation by KCl of stomatal opening in isolated epidermal strips of Vicia faba was examined. In dark + normal air the opening response was maximal at 100 mm KCl while in light + CO₂-free air it was maximal at about 10 mm KCl. CO₂-free air was more influential than light in reducing the KCl concentration required for maximal opening. K^{was essential while Cl}^{seemed to be of secondary importance in these processes.}The use of ^Rb^{as a tracer for K}^{showed that the increase in stomatal aperture under various conditions was well correlated with K}^{uptake. The estimated amount of K}^{taken up by guard cells, along with a counter ion, was sufficient to account for the changes in solute potential associated with opening. It is suggested that the absorption of extracellular solutes, such as K}^{, may be the primary mechanism of stomatal opening. Both opening and K}^{absorption are stimulated by light + CO}₂-free air.The increase in stomatal aperture was also well correlated with the decrease in stainable starch in guard cells under all conditions. It is suggested that this is a secondary change, although perhaps closely linked to K^absorption.

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