Availability of Chloride Affects the Balance between Potassium Chloride and Potassium Malate in Guard Cells of <em>Vicia faba</em> L. <sup><a href="#fn1" rid="fn1" class=" fn">1</a></sup>
Abstract
Electron probe microanalysis for K and Cl and enzymic determination of malate were performed on epidermal strips of Vicia faba L. which had been incubated with 0.1 equivalent of K per liter in the absence or presence of Cl. In the absence of Cl, iminodiacetate, a presumed impermeant zwitterion, served as anion. With no Cl in the medium, 91% of the K imported into the guard cells during stomatal opening was neutralized by malate production; import of Cl (presumably from the rest of the epidermal tissue) contributed 6%. In the presence of Cl, 50% of the necessary negative charges were provided by malate synthesis, 45% by Cl import. Stomatal opening was not obviously affected by the chloride concentration in the incubation medium, but malate production declined roughly linearly with the logarithm of [Cl] between 10 and 10 equivalent per liter.
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