A one-pot synthesis of solvophobic bisdodecylaminepalladium(II) chloride (complex 1) was performed. Complex 1 was characterized using X-ray crystallography and other techniques, namely, mass spectrometry, Fourier transform infrared, NMR, elemental analysis, etc. A multifaceted approach was taken to explore the potential applications of complex 1. The micellization ability of complex 1 was estimated using conductivity method in n-alcohols. The metallomicelles are formed in alcohols, and the process is thermodynamically spontaneous in nature. Using complex 1 as precursor, palladium (Pd) nanoparticles were fabricated using two-phase redox method, where reduction is being performed in core of metallomicelles formed by complex 1 in dichloromethane (DCM). The micellization in DCM is confirmed by small-angle X-ray scattering (SAXS). The SAXS measurements reveal that the micellar of core 4-5 nm is being formed, which further controls the size of nanoparticle. This approach was advantageous in terms of size control, methodology, and yield. Pd nanoparticles were characterized using transmission electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, and UV-visible spectroscopy and were also screened for bovine serum albumin interactions. Complex 1 and Pd nanoparticles were found to possess antimicrobial property with broad spectrum and are active against bacteria and fungi. The cytotoxicity analyses were performed over healthy cells (Vero cell lines extracted from kidney of green monkey), and the results reveal IC50 value of 10 μg/mL for complex 1.