Lilium brownii F. E. Brown var. viridulum Baker is a well-known and very important traditional Chinese medicine. It has been used as sedative, antitussive, anti-inflammatory or as a nutrient. Five compounds were isolated from the bulbs of Lilium brownii. On the basis of spectroscopic analysis (IR, FAB-MS, 1HNMR, 13CNMR, DEPT, HMQC, HMBC) and their physicochemical properties, they were identified as beta-sitosterol (I), daucosterol (II), n-butyl-beta-D-fructopyranoside (III), 26-O-beta-D-glucopyranosyl-3 beta, 26-dihydroxy-5-cholesten-16, 22-dioxo-3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside (IV), 26-O-beta-D-glucopyranosyl-3beta, 26-dihydroxy cholestan-16, 22-dioxo-3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside(V). Among them, I, II and III were isolated from this plant for the first time, IV and V are new compounds.