Abstract:

Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.

Open in

PUBMED | DOI | PMC | Google Scholar | Wikipedia

Relations:

Content

Citations

(1)

Diseases

(1)

Conditions

(1)

Chemicals

(2)

Genes

(2)

Organisms

(1)

Processes

(2)

Affiliates

(1)

Expression of the protective antigen for PEDV in transgenic duckweed, <em>Lemna minor</em>

Pyung Lim+2 authors

Abstract

Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.

Additional key words: aquatic plant, porcine diarrhea, spike protein, transformation, vaccine

^{Subtropical Horticulture Research Institute, Jeju National University, Jeju, 690-756 Korea}

^{Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806 Korea}

^{Research Institute for Basic Sciences, Jeju National University, Jeju, 690-756 Korea}

^{Department of Science Education, Jeju National University, Jeju, 690-756 Korea}

^{Faculty of Biotechnology, Jeju National University, Jeju, 690-756 Korea}

Hyo-Yeon Lee, Email: rk.ca.ujehc@noeyoyh.

Contributor Information.

^{Corresponding author.}

Received 2011 Jan 5; Accepted 2011 Jun 23.

This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

Footnotes

These authors are contributed equally to this work.

Footnotes

Contributor Information

Hyo-Yeon Lee, Email: rk.ca.ujehc@noeyoyh.

Suk Weon Kim, Email: rk.er.bbirk@wsmik.

Contributor Information

Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.

Learn More