Evaluation of Antidiabetic and Antioxidant Potential of Hydromethanolic Seed Extract of <em>Datura stramonium</em> Linn (Solanaceae)

^{Department of Pharmacy, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia}

Correspondence: Gedefaw Getnet Amare Wollo University, Dessie, 1145, Ethiopia, Phone: Tel +25 1911737428, Email gedefawg39@gmail.com

Received 2020 Apr 16; Accepted 2020 Jun 9.

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Abstract

Background

Nature has gifted a variety of phytochemicals having a potential effect against diabetes mellitus. Datura stramonium has been used as a remedy for the treatment of diabetes mellitus. The study aimed to determine the in vivo antidiabetic potential of hydromethanolic seed extract of the plant.

Methods

Dried seeds of Datura stramonium were macerated in hydromethanol. Three doses (100, 200, and 400 mg/kg) of the seed extract were given orally to normoglycemic, glucose-loaded, and Streptozocin-induced diabetic mice. Diphenyl-1-picrylhydrazine (DPPH) assay was employed to determine antioxidant activity of the seed extract.

Results

All doses of hydromethanolic seed extract of D. stramonium were devoid of any significant hypoglycemic effect in normoglycemic mice compared to the negative control group. Acute glucose reduction was significant (P<0.05 at 100, P<0.01 at 200 and 400 mg/kg) with respect to negative control in oral glucose-loaded mice. All doses of seed extract significantly (P<0.0l) reduced blood glucose level on weeks 1 and 2 in STZ-induced daily-treated diabetic mice. The seed extract at the doses of 200 and 400 mg/kg significantly (P<0.05) improved the body weight of diabetic mice on weeks 1 and 2. A low (100 mg/kg) dose of the seed extract delayed and significantly (P<0.05) increased body weight of mice on week 2 compared to negative control. The finding showed that the antioxidant activity of the hydromethanolic seed extract was concentration dependent and comparable with ascorbic acid. IC50 of the seed extract and ascorbic acid was found to be 11.95 and 5.07 mg/mL, respectively.

Conclusion

The findings of the study showed that hydromethanolic seed extract of Datura stramonium endowed significant antihyperglycemic and antioxidant activity.

Keywords: diabetes, Streptozotocin, Datura stramonium, antioxidant, mice

Abstract

Notes: +, present; –, absent.

Note: Values of % inhibition of DPPH free radical is described as mean±standard error of the mean.

Abbreviations: AA, ascorbic acid; DPPH, 2.2-diphenyl-1-picrylhydrazine; IC, inhibitory concentration; SE, seed extract.

Notes: Each data describes as mean±standard error of the mean, n=6; ^{compared to negative control;}^{to 100 mg/kg;}^{to 5 mg/kg GB;}^P<0.05; ^P<0.001.

Abbreviations: BGL, blood glucose level; GB, glibenclamide; NC, negative control; SE, seed extract.

Notes: Each data described as mean±standard error of the mean, n=6; ^{compared to negative control;}^P<0.01; ^P<0.001.

Abbreviations: BGL, blood glucose level; GB, glibenclamide; NC, negative control; SE, seed extract.

Notes: Each data described as mean±standard error of the mean, n=6; ^{compared to negative control;}^P<0.05; ^P<0.01.

Abbreviations: GB, glibenclamide; NC, negative control; SE, seed extract.

Acknowledgments

We would like to acknowledge Wollo University for providing the necessary facilities in this study.

Acknowledgments

Funding Statement

Not applicable.

Funding Statement

References

1. Skyler JS, Bakris GL, Bonifacio E, et al. Differentiation of diabetes by pathophysiology, natural history, and prognosis. Diabetes. 2017;66(2):241–255. doi:10.2337/db16-0806 ] [
2. Alberti KGMM, Zimmet P. Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus. Provisional report of a WHO consultation. Diabet Med. 1998;15(7):539–553. doi:10.1002/(SICI)1096-9136(199807)15:7<539::AID-DIA668>3.0.CO;2-S [] [[PubMed]
3. Schofield JD, Liu Y, Rao-Balakrishna P, Malik RA, Soran H. Diabetes dyslipidemia. Diabetes Ther. 2016;7(2):203–219. doi:10.1007/s13300-016-0167-x ] [
4. Vijayaraghavan K. Treatment of dyslipidemia in patients with type 2 diabetes. Lipids Health Dis. 2010;9(1):144. doi:10.1186/1476-511X-9-144 ] [
5. Ormazabal V, Nair S, Elfeky O, et al. Association between insulin resistance and the development of cardiovascular disease Cardiovasc. Diabetol. 2018;17:122.
6. International Diabetes Federation. IDF Diabetes Atlas. 8th ed. Belgium: Brussels; 2017. [PubMed]
7. Sharma BR, Kim H, Rhyu D. Caulerpa lentillifera extract ameliorates insulin resistance and regulates glucose metabolism in C57BL/KsJ-db/db mice via PI3K/AKT signaling pathway in myocytes. J Transl Med. 2015;13:62. doi:10.1186/s12967-015-0412-5 ] [
8. Janda E, Lascala A, Martino C, et al. Molecular mechanisms of lipid-and glucose-lowering activities of bergamot flavonoids. Pharma Nutrition. 2016;4:S8–S18. doi:10.1016/j.phanu.2016.05.001 [[PubMed]
9. Hung HY, Qian K, Morris-Natschke SL, Hsu CS, Lee KH. Recent discovery of plant-derived anti-diabetic natural products. Nat Prod Rep. 2012;29(5):580–606. [[PubMed]
10. D A, Geleta G, Bacha K, Abdissa N. Phytochemical investigation of Aloe pulcherrima roots and evaluation for its antibacterial and antiplasmodial activities. PLoS One. 2018;12(3):e0173882.
11. Yousef F, Yousef N, Mansour O, Herbali J. Metformin: a unique herbal origin medication. Glob J Med Res. 2017. [PubMed]
12. Goel PK. Novel antidiabetic furostanolic saponin rich (fsr) fraction from fenugreek seeds. Google Patents; 2012. [PubMed]
13. Toma A, Makonnen E, Mekonnen Y, Debella A, Adisakwattana S. Antidiabetic activities of aqueous ethanol and n-butanol fraction of Moringa stenopetala leaves in streptozotocin-induced diabetic rats. BMC Complement Altern Med. 2015;15(1):242. doi:10.1186/s12906-015-0779-0 ] [
14. Abo-Youssef AM, Messiha BA. Beneficial effects of Aloe vera in treatment of diabetes: comparative in vivo and in vitro studies. Bull Fac Pharm Cairo Univ. 2013;51(1):7–11. doi:10.1016/j.bfopcu.2012.03.002 [[PubMed]
15. HF H, Hajiaghaee R, Dabaghian FH. Anti-hyperglycemic and anti-hypercholesterolemic effects of Aloe vera leaf gel in hyperlipidemic type 2 diabetic patients: a randomized double-blind placebo-controlled clinical trial. Planta Med. 2012;78(04):311–316. doi:10.1055/s-0031-1280474 [] [[PubMed]
16. Samuels J. Biodiversity of food species of the Solanaceae family: a preliminary taxonomic inventory of subfamily Solanoideae. Resources. 2015;4(2):277–322. doi:10.3390/resources4020277 [[PubMed]
17. Gebhardt C. The historical role of species from the Solanaceae plant family in genetic research. Theor Appl Genet. 2016;129(12):2281–2294. doi:10.1007/s00122-016-2804-1 ] [
18. Al-Snafi AE. Medical importance of Datura fastuosa (syn: Datura metel) and Datura stramonium-A review. IOSR J Pharm. 2017;7(2):43–58. doi:10.9790/3013-0702014358 [[PubMed]
19. Soni P, Siddiqui AA, Dwivedi J, Soni V. Pharmacological properties of Datura stramonium L. as a potential medicinal tree: an overview. Asian Pac J Trop Biomed. 2012;2(12):1002–1008. doi:10.1016/S2221-1691(13)60014-3 ] [
20. Miraj S. Datura stramonium: an updated review. Der Pharma Chemica. 2016;8(17):253–257. [PubMed]
21. Lewis O, Nieman E, Munz A. Origin of amino acids in Datura stramonium seeds. Ann Bot. 1970;34(4):843–848. doi:10.1093/oxfordjournals.aob.a084415 [[PubMed]
22. Rashid S, Ahmad M, Zafar M, et al. Ethnopharmacological evaluation and antioxidant activity of some important herbs used in traditional medicines. J Tradit Chin Med. 2016;36(5):689–694. doi:10.1016/S0254-6272(16)30091-7 [] [[PubMed]
23. Baynesagne S, Berhane N, Sendeku W, Ai L. Antibacterial activity of Datura stramonium against standard and clinical isolate pathogenic microorganisms. J Med Plants Res. 2017;11(31):501–506. doi:10.5897/JMPR2017.6381 [[PubMed]
24. Belayneh M, Birhanu Z, Birru M, Getenet G. Evaluation of in vivo antidiabetic, antidyslipidemic and in vitro antioxidant activities of hydromethanolic root extract of Datura stramonium L. (Solanaceae). J Exp Pharmacol. 2019;11:29–38. doi:10.2147/JEP.S192264 ] [
25. Mehrabadi M, Saadati F, Mahmudvand M. α-Amylase activity of stored products insects and its inhibition by medicinal plant extracts. J Agr Sci Tech. 2011;13:1173–1182. [PubMed]
26. Meresa A, Gemechu W, Basha H, et al. Herbal medicines for the management of diabetic mellitus in Ethiopia and Eretria including their phytochemical constituents. Am J Adv Drug Deliv. 2017;5(1):40–58. doi:10.21767/2321-547X.1000011 [[PubMed]
27. Janet CG, Joseph T, Bielitzki L, Ann C. Guide for the Care and Use of Laboratory Animals. Eighth ed. Washington, DC: Institute of Laboratory Animal Resources, National Research Council; 2010:248. [PubMed]
28. Pandey A, Tripathi S. Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. J Pharmacogn Phytochem. 2014;2(5). [PubMed]
29. Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Int Pharm Sci. 2011;1(1):98–106. [PubMed]
30. OECD/OCDE. OECD guideline for the testing of chemicals: acute oral toxicity; up-and-down procedure (UDP). No 425 OECD; 2008. [PubMed]
31. MacDonald‐Wicks LK, Wood LG, Garg ML. Methodology for the determination of biological antioxidant capacity in vitro: a review. J Sci Food Agric. 2006;86(13):2046–2056. doi:10.1002/jsfa.2603 [[PubMed]
32. Etuk E. Animal models for studying diabetes mellitus. ABJNA. 2010;1(2):130–134. [PubMed]
33. MMPC. Streptozotocin-induced type 1 diabetes model. Mouse Metabolic Phenotyping Centers protocols.2013.
34. Sulyman A, Akolade J, Sabiu S, Aladodo R, Muritala H. Antidiabetic potentials of ethanolic extract of Aristolochia ringens (Vahl.) roots. J Ethnopharmacol. 2016;182:122–128. doi:10.1016/j.jep.2016.02.002 [] [[PubMed]
35. Baquer NZ, Kumar P, Taha A, Kale R, Cowsik S, McLean P. Metabolic and molecular action of Trigonella foenum-graecum (fenugreek) and trace metals in experimental diabetic tissues. J Biosci. 2011;36(2):383–396. doi:10.1007/s12038-011-9042-0 [] [[PubMed]
36. Tamiru W, Engidawork E, Asres K. Evaluation of the effects of 80% methanolic leaf extract of Caylusea abyssinica (fresen.) fisch. & Mey. on glucose handling in normal, glucose loaded and diabetic rodents. BMC Complement Altern Med. 2012;12(1):151. doi:10.1186/1472-6882-12-151 ] [
37. Demoz MS, Gachoki KP, Mungai KJ, Negusse BG. Evaluation of the anti-diabetic potential of the methanol extracts of aloe camperi, meriandra dianthera and a polyherb. J Diabetes Mellit. 2015;5(04):267. doi:10.4236/jdm.2015.54033 [[PubMed]
38. Zhang Y, Feng F, Chen T, et al. Antidiabetic and antihyperlipidemic activities of Forsythia suspensa (Thunb.) Vahl (fruit) in streptozotocin-induced diabetes mice. J Ethnopharmacol. 2016;192(2562):56–263. doi:10.1016/j.jep.2016.07.002 [] [[PubMed]
39. Sharma S, Choudhary M, Bhardwaj S, Choudhary N, Rana AC. Hypoglycemic potential of alcoholic root extract of Cassia occidentalis Linn. in streptozotocin induced diabetes in albino mice. Bull Fac Pharm Cairo Univ. 2014;52(2):211–217. doi:10.1016/j.bfopcu.2014.09.003 [[PubMed]
40. Rajurkar B. Phyto-pharmacological investigations of Clerodendrum infortunatum Gartn. Int Res J Pharm. 2011;2:130–132. [PubMed]
41. Tesfaye A, Makonnen E, Gedamu S. Hypoglycemic and antihyperglycemic activity of aqueous extract of Justicia Schimperiana leaves in normal and streptozotocin-induced diabetic mice. Int. J Pharm Sci Res. 2016;7(02):110–113. [PubMed]
42. Hammeso W, Emiru Y, Ayalew G, Kahaliw W. Antidiabetic and antihyperlipidemic activities of the leaf latex extract of Aloe megalacantha baker (Aloaceae) in streptozotocin-induced diabetic model. Evid Based Complement Alternat Med. 2019;2019:1–9. doi:10.1155/2019/8263786 ] [
43. Otsuka HPurification by solvent extraction using partition coefficient In: Sarker D, Latif Z, Gray A, editors. Methods in Biotechnology Natural Products Isolation. Human Press; 2006:269–273. [PubMed]
44. Moon J-K, Shibamoto T. Antioxidant assays for plant and food components. J Agric Food Chem. 2009;57(5):1655–1666. doi:10.1021/jf803537k [] [[PubMed]
45. Misbah H, Aziz AA, Aminudin N. Antidiabetic and antioxidant properties of Ficus deltoidea fruit extracts and fractions. BMC Complement Altern Med. 2013;13:118. doi:10.1186/1472-6882-13-118 ] [
46. Belayneh Y, Birru EM, Ambikar D. Evaluation of hypoglycemic, antihyperglycemic and antihyperlipidemic activities of 80% methanolic seed extract of Calpurnia aurea (Ait.) Benth (Fabaceae) in mice. J Exp Pharmacol. 2019;11:73. doi:10.2147/JEP.S212206 ] [
47. Deeds M, Anderson J, Armstrong A, et al. Single dose streptozotocin-induced diabetes: considerations for study design in islet transplantation models. Lab Anim. 2011;45(3):131–140. doi:10.1258/la.2010.010090 ] [
48. Šoltésová D, Herichová I. On the mechanisms of diabetogenic effects of alloxan and streptozotocin. Diabetol Metab Endokrinologie. 2011;14:130–138. [PubMed]
49. Krishna Murthy B, Nammi S, Kota MK, Krishna Rao RV. Evaluation of hypoglycemic and antihyperglycemic effects of Datura metel (Linn.) seeds in normal and alloxan-induced diabetic rats. J Ethnopharmacol. 2004;91(1):95–98. doi:10.1016/j.jep.2003.12.010 [] [[PubMed]