Plant Physiol 50(5): 531-535
Location of Glycoproteins That Contain Glucosamine in Plant Tissues <sup><a href="#fn1" rid="fn1" class=" fn">1</a></sup>
Abstract
When radioactive d-glucosamine is provided to Acer pseudoplatanus cells in liquid culture in order to label those glycoproteins that contain amino sugars, it is incorporated predominantly into a crude cell wall fraction. This observation was confirmed histologically by preparing autoradiographs of thin tissue sections from plasmolyzed cells. Highly purified cell wall material from unlabeled cells has also been shown to contain small amounts of glucosamine. Similarly, about one-half of the amino sugar recovered from cultured cells of Nicotiana tabacum is present in their cell walls. In corn roots, however, the labeled glycoproteins that are formed after glucosamine incorporation are predominantly cytoplasmic and not deposited outside the protoplast.
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- BOAS NF. Method for the determination of hexosamines in tissues. J Biol Chem. 1953 Oct;204(2):553–563. [PubMed] [Google Scholar]
- Coulter HD. Rapid and improved methods fr embedding biological tissues in Epon 812 and Araldite 502. J Ultrastruct Res. 1967 Oct 31;20(5):346–355. [PubMed] [Google Scholar]
- Eylar EH. On the biological role of glycoproteins. J Theor Biol. 1966 Jan;10(1):89–113. [PubMed] [Google Scholar]
- Filner P. Semi-conservative replication of DNA in a higher plant cell. Exp Cell Res. 1965 Aug;39(1):33–39. [PubMed] [Google Scholar]
- Heath MF, Northcote DH. Glycoprotein of the wall of sycamore tissue-culture cells. Biochem J. 1971 Dec;125(4):953–961.[PMC free article] [PubMed] [Google Scholar]
- Kalb AJ. The separation of three L-fucose-binding proteins of Lotus tetragonolobus. Biochim Biophys Acta. 1968 Dec 3;168(3):532–536. [PubMed] [Google Scholar]
- KOHN P, WINZLER J, HOFFMAN RC. Metabolism of D-glucosamine and N-acetyl-D-glucosamine in the intact rat. J Biol Chem. 1962 Feb;237:304–308. [PubMed] [Google Scholar]
- Lamport DT. The isolation and partial characterization of hydroxyproline-rich glycopeptides obtained by enzymic degradation of primary cell walls. Biochemistry. 1969 Mar;8(3):1155–1163. [PubMed] [Google Scholar]
- OTA S, MOORE S, STEIN WH. PREPARATION AND CHEMICAL PROPERTIES OF PURIFIED STEM AND FRUIT BROMELAINS. Biochemistry. 1964 Feb;3:180–185. [PubMed] [Google Scholar]
- Roberts RM. The incorporation of D-glucosamine-14C into root tissues of higher plants. Plant Physiol. 1970 Mar;45(3):263–267.[PMC free article] [PubMed] [Google Scholar]
- Roberts RM, Butt VS. Patterns of incorporation of pentose and uronic acid into the cell walls of maize root-tips. Exp Cell Res. 1968 Aug-Sep;51(2-3):519–530. [PubMed] [Google Scholar]
- Roberts RM, Connor AB, Cetorelli JJ. The formation of glycoproteins in tissues of higher plants. Specific labelling with D-(1- 14 C)glucosamine. Biochem J. 1971 Dec;125(4):999–1008.[PMC free article] [PubMed] [Google Scholar]
- Sadava D, Chrispeels MJ. Cell wall protein in plants: autoradiographic evidence. Science. 1969 Jul 18;165(3890):299–300. [PubMed] [Google Scholar]
- Shannon LM, Kay E, Lew JY. Peroxidase isozymes from horseradish roots. I. Isolation and physical properties. J Biol Chem. 1966 May 10;241(9):2166–2172. [PubMed] [Google Scholar]
- STARK GR, DAWSON CR. On the accessibility of sulfhydryl groups in ascorbic acid oxidase. J Biol Chem. 1962 Mar;237:712–716. [PubMed] [Google Scholar]
- Takahashi T, Ramachandramurthy P, Liener IE. Some physical and chemical properties of a phytohemagglutinin isolated from Phaseolus vulgaris. Biochim Biophys Acta. 1967 Jan 18;133(1):123–133. [PubMed] [Google Scholar]