Abstract:

A study was done on the effects of various factors on carbohydrate inhibition of flowering and on in vitro activity of glucose 6-phosphate dehydrogenase in Lemna perpusilla 6746 grown on dilute Hutner's medium in short days. Autoclaving decreased the flower inhibitory effect of sucrose but increased the effects of glucose and fructose. Sucrose inhibition was mimicked by CO(2) and was partially reversed by agitation of the cultures. Inhibition by sucrose was also partially reversed by ATP and intermediates of the tricarboxylic acid cycle, the glycolytic pathway, or the pentose phosphate pathway. Tartaric acid was inactive. Glycine, l-alanine, l-aspartate, l-asparagine, l-serine, l-glutamate, and l-glutamine were active, whereas l-cysteine, l-arginine, l-lysine, l-leucine, l-isoleucine, l-proline, l-tyrosine, l-tryptophane, and l-phenylalanine were not. Incubation of cultures on distilled water during a single inductive long night prevented flowering. This inhibition was partially reversed by l-alanine and glucose 6-phosphate. There was a correlation between carbohydrate inhibition of flowering and enhancement of glucose 6-phosphate dehydrogenase. Possible mechanisms for the carbohydrate inhibition of flowering are discussed.

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Inhibitory Effect of Carbohydrate on Flowering in <em>Lemna perpusilla</em>

H Posner

Abstract

A study was done on the effects of various factors on carbohydrate inhibition of flowering and on in vitro activity of glucose 6-phosphate dehydrogenase in Lemna perpusilla 6746 grown on dilute Hutner's medium in short days. Autoclaving decreased the flower inhibitory effect of sucrose but increased the effects of glucose and fructose. Sucrose inhibition was mimicked by CO₂ and was partially reversed by agitation of the cultures. Inhibition by sucrose was also partially reversed by ATP and intermediates of the tricarboxylic acid cycle, the glycolytic pathway, or the pentose phosphate pathway. Tartaric acid was inactive. Glycine, l-alanine, l-aspartate, l-asparagine, l-serine, l-glutamate, and l-glutamine were active, whereas l-cysteine, l-arginine, l-lysine, l-leucine, l-isoleucine, l-proline, l-tyrosine, l-tryptophane, and l-phenylalanine were not. Incubation of cultures on distilled water during a single inductive long night prevented flowering. This inhibition was partially reversed by l-alanine and glucose 6-phosphate. There was a correlation between carbohydrate inhibition of flowering and enhancement of glucose 6-phosphate dehydrogenase. Possible mechanisms for the carbohydrate inhibition of flowering are discussed.

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Gamborg OL, Shyluk JP. The culture of plant cells with ammonium salts as the sole nitrogen source. Plant Physiol. 1970 May;45(5):598–600.[PMC free article] [PubMed] [Google Scholar]
Halaban R, Hillman WS. Response of Lemma perpusilla to Periodic Transfer to Distilled Water. Plant Physiol. 1970 Nov;46(5):641–644.[PMC free article] [PubMed] [Google Scholar]
Hillman WS, Posner HB. Ammonium Ion and the Flowering of Lemna perpusilla. Plant Physiol. 1971 Apr;47(4):586–587.[PMC free article] [PubMed] [Google Scholar]
HOLZER H. Regulation of carbohydrate metabolism by enzyme competition. Cold Spring Harb Symp Quant Biol. 1961;26:277–288. [PubMed] [Google Scholar]
Posner HB. Inhibitory Effect of Carbohydrate on Flowering in Lemna perpusilla. I. Interaction of Sucrose With Calcium and Phosphate Ions. Plant Physiol. 1969 Apr;44(4):562–566.[PMC free article] [PubMed] [Google Scholar]
Posner HB. Inhibitory Effect of Carbohydrate on Flowering in Lemna perpusilla: II. Reversal by Glycine and l-Aspartate. Correlation with Reduced Levels of beta-Carotene and Chlorophyll. Plant Physiol. 1970 Jun;45(6):687–690.[PMC free article] [PubMed] [Google Scholar]

^{Department of Biological Sciences, State University of New York, Binghamton, New York 13901}

^{This work was supported by National Science Foundation Grant GB-12955.}

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Abstract

A study was done on the effects of various factors on carbohydrate inhibition of flowering and on in vitro activity of glucose 6-phosphate dehydrogenase in Lemna perpusilla 6746 grown on dilute Hutner's medium in short days. Autoclaving decreased the flower inhibitory effect of sucrose but increased the effects of glucose and fructose. Sucrose inhibition was mimicked by CO₂ and was partially reversed by agitation of the cultures. Inhibition by sucrose was also partially reversed by ATP and intermediates of the tricarboxylic acid cycle, the glycolytic pathway, or the pentose phosphate pathway. Tartaric acid was inactive. Glycine, l-alanine, l-aspartate, l-asparagine, l-serine, l-glutamate, and l-glutamine were active, whereas l-cysteine, l-arginine, l-lysine, l-leucine, l-isoleucine, l-proline, l-tyrosine, l-tryptophane, and l-phenylalanine were not. Incubation of cultures on distilled water during a single inductive long night prevented flowering. This inhibition was partially reversed by l-alanine and glucose 6-phosphate. There was a correlation between carbohydrate inhibition of flowering and enhancement of glucose 6-phosphate dehydrogenase. Possible mechanisms for the carbohydrate inhibition of flowering are discussed.

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