Effect of hydroalcoholic extract of <em>Hibiscus rosa sinensis</em> Linn. leaves in experimental colitis in rats

Amit Kandhare

Kiran Raygude

Pinaki Ghosh

Arvindkumar Ghule

Tejas Gosavi

Sachin Badole

Subhash Bodhankar

Objective

To elucidate the ameliorative effect of hydroalcoholic extract of leaves of Hibiscus rosa sinensis (HRS) in acetic acid induced experimental colitis in male wistar rats.

Methods

The animals were administered with 2 mL acetic acid (4%) via intra rectal. The animals were divided into various treatment groups (n=6). Prednisolone was used as standard drug and HRS was administered at a dose of 50, 100 and 200 mg/kg p.o. The control group of animals received 1 mL of vehicle (distilled water). Ulcer area, ulcer index, spleen weight, colon weight to length ratio, macroscopic score, haematological parameters, colonic superoxide dismutase (SOD), glutathione (GSH), myeloperoxidase (MPO), malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), nitric oxide (NO) and histological changes were recorded after the treatment regimen of 11 days.

Results

Intrarectal instillation of acetic acid caused enhanced ulcer area, ulcer index, spleen weight, colon weight to length ratio, colonic MPO, MDA, NO and TNF-α It caused significant decreased level of SOD and GSH. Pretreatment with HRS for 7 days exhibited significant effect in lowering of oxidative stress, colonic NO, TNF-α and elevation of SOD and GSH at a dose of 100 and 200 mg/kg in acetic acid induced colitis.

Conclusions

The present investigation demonstrates HRS is of potent therapeutic value in the amelioration of experimental colitis in laboratory animals by inhibiting the proinflammatory mediator like NO and TNF-α.

^{Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, Maharashtra, 411038, India}

^{Department of Homeopathic Pharmacy, Homeopathic Medical College, Bharati Vidyapeeth Deemed University, Dhankawadi, Katraj, Pune, Maharashtra, 411043, India}

*Corresponding author: Dr. Bodhankar SL, Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Pune-411038 Maharashtra, India. Tel: +91-20-25437237, +91-20-25437229, Fax: +91-20-25439383, E-mail: moc.oohay@hdobs

Received 2011 Jul 25; Revised 2011 Sep 5; Accepted 2011 Sep 27.

Abstract

Objective

To elucidate the ameliorative effect of hydroalcoholic extract of leaves of Hibiscus rosa sinensis (HRS) in acetic acid induced experimental colitis in male wistar rats.

Methods

Results

Conclusions

Keywords: Acetic acid, Hibiscus rosa sinensis, Inflammatory bowel disease, Inflammatory cytokine, Nitrite/nitrate, Oxidative stress, Ulcerative colitis, Prednisolone

Abstract

Acknowledgments

The authors would like acknowledge Dr. SS Kadam, Vice-Chancellor and Dr. KR Mahadik, Principal, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, India, for providing necessary facilities to carry out the study. We are also thankful to the All India Council of Technical and Education (AICTE), India (Ref No. PG/GATE-SCM/2004-2005/G-39, Dated: 11/02/2011) for financial support by awarding GATE Scholarship to one of the author Mr. Kandhare Amit for the research work.

Acknowledgments

Footnotes

Foundation Project: Supported by the All India Council of Technical and Education (AICTE), India (Grant No. PG/GATE-SCM/2004-2005/G-39, Dated: 11/02/2011).

Conflict of interest statement: We declare that we have no conflict of interest.

Footnotes

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