Evaluation of antifungal activity of free fatty acids methyl esters fraction isolated from Algerian <em>Linum usitatissimum</em> L. seeds against toxigenic <em>Aspergillus</em>

Amrouche Abdelillah

Benmehdi Houcine

Dalile Halima

Chabane Meriem

Objective

The aim of this study was to evaluate the antifungal activity of the major fraction of fatty acids methyl esters (FAMEs) isolated from Linum usitatissimum L. seeds oil collected from Bechar department (Algeria).

Methods

The assessment of antifungal activity was carried out in terms of percentage of radial growth on solid medium (potatoes dextrose agar PDA) and biomass growth inhibition on liquid medium (potatoes dextrose broth PDB) against two fungi.

Results

The FAMEs was found to be effective in inhibiting the radial mycelial growth of Aspergillus flavus more than Aspergillus ochraceus on all tested concentrations. The highest antifungal index was found to be (54.19%) compared to Aspergillus ochraceus (40.48%). The results of the antifungal activity of the FAMEs inhibition of biomass on liquid medium gave no discounted results, but this does not exclude the antifungal activity.

Conclusions

We can assume that the observed antifungal potency may be due to the abundance of linoleic and α-linolenic acids in linseed oil which appears to be promising to treat fungal infections, storage fungi and food spoilage in food industry field.

*Corresponding author: Benmehdi Houcine, Faculty of Sciences and technology, Department of Technology, University of Bechar 08000 Algeria. Tel: 00213779517854, E-mail: rf.oohay@09neb_h

Received 2013 Apr 5; Accepted 2013 May 20.

Abstract

Objective

Methods

Results

Conclusions

Keywords: Antifungal activity, FAMEs, Linum usitatissimum L., Extraction, Aspergillus

Abstract

Acknowledgments

This work received supplied assistance from university of Bechar and the Algerian Ministry of the higher education and scientific research. This work has been funded by a university research project (PRU project approved in 2011/2013 under the code F03820100015). We are great to our co-workers who supplied fungal isolates and provided useful suggestions and information's for this study.

Acknowledgments

Notes

Comments

Background

A. flavus and A. ochraceus are able to deteriorate food, inducing organoleptic and chemical deteriorations with serious risks of intoxications for the consumers. Produced A. flavus of aflatoxine group's substances are toxic and carcinogenic (disease X of turkeys and other livestock). A. ochraceus produced of the ochratoxine, this mycotoxin is the cause of human renal disease, and easily contaminates the cereal grains stored in the mould.

Research frontiers

The means of fight against the moulds are numerous: A rigorous hygiene, physical agents like the temperature or radiations but cannot be used in a universal way, certain chemicals (fungicidal) can also be used, but harmful for the man, very recently lactic Bacillus and other bacteria can play a determining role. This work is interested has the action of the FAME extradited of the plant: L. usutatissimum. Indeed, several work showed that certain parts of the plants has inhibiting properties with respect to the bacteria, mushrooms and insects without side effects.

Related reports

The objective of this study is to evaluate the antiphonic activity of the fatty acids (FAME) of seeds of L. usutatissimum Exit of the Bechar (Algérie) area. Methodology is well described, the means implemented make it possible to control an experimental work well on the living organisms: Identification of the moulds and quantification of the activity, the latter was evaluated in terms of percentage of the radial growth on solid medium and by the inhibition of the growth on liquid medium, with respect to two moulds: Aspergillus F was shown more sensitive with a rate of 54.19% compared to A. okraceus (40.48%), but the results on the biomass seems to be can convincing.

Innovations and breakthroughs

Fatty acids from the plant have antifungal activity may be quite large and the natural products label food preservation, but deeper studies should be conducted to confirm that they do not present a danger to the living; it would be interesting to see how they are working on these foods (spray coating ...).

Applications

The presence of producing toxin moulds must be taken with the serious one, they are carcinogenic molecules even has low dose since, not or with difficulty metabolize and thus accumulates in the organization, the prevention is essential in the silos and other places of storages which must be refractory mediums with the development of mushrooms. This plant and well of others of the area must undergo a screening for their metabolites have fine to replace: Chemicals and other radiations for the conservation of food.

Peer review

This contribution can be a remedy for the gravity and the abundance of the contamination of the foodstuffs. Indeed, FAMEs of seeds of L. usutatissimum, exit of the Bechar (Algeria) area, showed an appreciable activity about (54.19% and 40.48%) successively on Aspergillus F and A. oracles. This antifungal capacity can be due to the abundance of the linoleic and linolenic acids in the linseed oil which seems promising to treat the fungi infections, the mushrooms of storage and deterioration of food in the field of food industry.

Notes

Footnotes

Foundation Project: Supported from the Algerian Ministry of the higher education and scientific research, PRU project approved in 2011/2013 under the code F03820100015).

Conflict of interest statement: We declare that we have no conflict of interest.

Footnotes

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