Abstract:

Epidermal strips of Vicia faba were floated on 10 millimolar KCl at various temperatures and for several time periods. The diameter of the stomatal aperture was determined microscopically and K(+) content was estimated and expressed as the per cent of the guard cell stained. Stomatal opening was associated with increased K(+) in guard cells, but the quantitative association was modified both by time and temperature. At low temperatures (0-20 C) there was a prolonged Spannungsphase while at higher temperatures (30-45 C) motorphase was exhibited. During the motorphase there was a rapid opening of the stomates which was highly correlated with K(+) influx. At treatment periods of 360 minutes and temperatures higher than 25 C there appeared to be a maintenance phase during which K(+) concentration of the guard cells decreased without an equivalent decrease in aperture.

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Relationship of Temperature to Stomatal Aperture and Potassium Accumulation in Guard Cells of <em>Vicia faba</em><sup><a href="#fn1" rid="fn1" class=" fn">1</a></sup>

C Rogers

Abstract

Epidermal strips of Vicia faba were floated on 10 millimolar KCl at various temperatures and for several time periods. The diameter of the stomatal aperture was determined microscopically and K^{content was estimated and expressed as the per cent of the guard cell stained. Stomatal opening was associated with increased K}^{in guard cells, but the quantitative association was modified both by time and temperature. At low temperatures (0-20 C) there was a prolonged}Spannungsphase while at higher temperatures (30-45 C) motorphase was exhibited. During the motorphase there was a rapid opening of the stomates which was highly correlated with K^{influx. At treatment periods of 360 minutes and temperatures higher than 25 C there appeared to be a maintenance phase during which K}^{concentration of the guard cells decreased without an equivalent decrease in aperture.}

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Selected References

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DeMichele DW, Sharpe PJ. An analysis of the mechanics of guard cell motion. J Theor Biol. 1973 Sep 14;41(1):77–96. [PubMed] [Google Scholar]
Fischer RA. Role of Potassium in Stomatal Opening in the Leaf of Vicia faba. Plant Physiol. 1971 Apr;47(4):555–558.[PMC free article] [PubMed] [Google Scholar]
Fischer RA, Hsiao TC. Stomatal Opening in Isolated Epidermal Strips of Vicia faba. II. Responses to KCl Concentration and the Role of Potassium Absorption. Plant Physiol. 1968 Dec;43(12):1953–1958.[PMC free article] [PubMed] [Google Scholar]
Humble GD, Hsiao TC. Light-dependent Influx and Efflux of Potassium of Guard Cells during Stomatal Opening and Closing. Plant Physiol. 1970 Sep;46(3):483–487.[PMC free article] [PubMed] [Google Scholar]
Humble GD, Raschke K. Stomatal opening quantitatively related to potassium transport: evidence from electron probe analysis. Plant Physiol. 1971 Oct;48(4):447–453.[PMC free article] [PubMed] [Google Scholar]
Sawhney BL, Zelitch I. Direct determination of potassium ion accumulation in guard cells in relation to stomatal opening in light. Plant Physiol. 1969 Sep;44(9):1350–1354.[PMC free article] [PubMed] [Google Scholar]

^{This work was supported by the Division of Environmental Biology of the National Science Foundation Grant DEB75-04108 to the Biosystems Research Division, Department of Industrial Engineering and by the Texas Agricultural Experiment Station, Texas A&M University.}

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Abstract

Epidermal strips of Vicia faba were floated on 10 millimolar KCl at various temperatures and for several time periods. The diameter of the stomatal aperture was determined microscopically and K^{content was estimated and expressed as the per cent of the guard cell stained. Stomatal opening was associated with increased K}^{in guard cells, but the quantitative association was modified both by time and temperature. At low temperatures (0-20 C) there was a prolonged}Spannungsphase while at higher temperatures (30-45 C) motorphase was exhibited. During the motorphase there was a rapid opening of the stomates which was highly correlated with K^{influx. At treatment periods of 360 minutes and temperatures higher than 25 C there appeared to be a maintenance phase during which K}^{concentration of the guard cells decreased without an equivalent decrease in aperture.}

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