Expression of a human lactoferrin cDNA in tobacco cells produces antibacterial protein(s).
Abstract
A suspension tobacco (Nicotiana tabacum L.) cell line was transformed to express human lactoferrin, an iron-binding glycoprotein. The transgenic calli produced a protein that was significantly smaller than the full-length lactoferrin protein. Total protein extracts made from transgenic tobacco callus exhibited much higher antibacterial activity than commercially available purified lactoferrin as determined by the decrease of colony-forming units when tested with four phytopathogenic species of bacteria. Introduction of the lactoferrin gene in crop plants may provide resistance against phytopathogenic bacteria.
Full Text
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arnold RR, Cole MF, McGhee JR. A bactericidal effect for human lactoferrin. Science. 1977 Jul 15;197(4300):263–265. [PubMed] [Google Scholar]
- Fromm ME, Morrish F, Armstrong C, Williams R, Thomas J, Klein TM. Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Biotechnology (N Y) 1990 Sep;8(9):833–839. [PubMed] [Google Scholar]
- Green MR, Pastewka JV. Lactoferrin is a marker for prolactin response in mouse mammary explants. Endocrinology. 1978 Oct;103(4):151–103. [PubMed] [Google Scholar]
- Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed] [Google Scholar]
- Liu YH, Teng CT. Characterization of estrogen-responsive mouse lactoferrin promoter. J Biol Chem. 1991 Nov 15;266(32):21880–21885. [PubMed] [Google Scholar]
- Logemann J, Schell J, Willmitzer L. Improved method for the isolation of RNA from plant tissues. Anal Biochem. 1987 May 15;163(1):16–20. [PubMed] [Google Scholar]
- Mitra A, An G. Three distinct regulatory elements comprise the upstream promoter region of the nopaline synthase gene. Mol Gen Genet. 1989 Jan;215(2):294–299. [PubMed] [Google Scholar]
- Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed] [Google Scholar]
- Tomita M, Bellamy W, Takase M, Yamauchi K, Wakabayashi H, Kawase K. Potent antibacterial peptides generated by pepsin digestion of bovine lactoferrin. J Dairy Sci. 1991 Dec;74(12):4137–4142. [PubMed] [Google Scholar]
- Ward PP, May GS, Headon DR, Conneely OM. An inducible expression system for the production of human lactoferrin in Aspergillus nidulans. Gene. 1992 Dec 1;122(1):219–223. [PubMed] [Google Scholar]
- Yamauchi K, Tomita M, Giehl TJ, Ellison RT., 3rd Antibacterial activity of lactoferrin and a pepsin-derived lactoferrin peptide fragment. Infect Immun. 1993 Feb;61(2):719–728.[PMC free article] [PubMed] [Google Scholar]
Abstract
A suspension tobacco (Nicotiana tabacum L.) cell line was transformed to express human lactoferrin, an iron-binding glycoprotein. The transgenic calli produced a protein that was significantly smaller than the full-length lactoferrin protein. Total protein extracts made from transgenic tobacco callus exhibited much higher antibacterial activity than commercially available purified lactoferrin as determined by the decrease of colony-forming units when tested with four phytopathogenic species of bacteria. Introduction of the lactoferrin gene in crop plants may provide resistance against phytopathogenic bacteria.