The objective of this study was to evaluate the antimicrobial effect of Agave fructans against the Salmonella Typhimurium in "in vitro" experiments. The result of the antimicrobial activity was 263.89 ± 0, 414.95 ± 12.83, 494.54 ± 13.88, 522.29 ± 0, 580.41 ± 14.92 AU for 10, 20, 30, 40 and 50% of Agave fructans (AF) respectively. In addition, there is a significant effect on the growth of the bacteria with all the percentages of AF evaluated (p < 0.001, R2 = 0.859) with respect to the control. The growth rate of Salmonella with 25% AF was statistically significant compared to the control (-0.7353 ± 0.586, 0.0079 ± 0.002 D.O./h, respectively; p > 0.01). Agave fructans could be an alternative to prevent the infections caused by Salmonella.