Effects of <em>Rheum tanguticum</em> polysaccharide on TNBS -induced colitis and CD4<sup>+</sup> T cells in rats

Li Liu

Zhi Wang

Chang Xu

Bo Pan

Li Liu, Zhi-Peng Wang, Qi-Bing Mei, Si-Yuan Zhou, Department of Pharmacology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Yin Long, Jia-Yun Liu, Department of Traditional Chinese Medicine, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Chang-Tai Xu, Editorial Department of Journal of Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Bo-Rong Pan, Department of Oncology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Qi-Bing Mei, Department of Pharmacology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. moc.liamtoh@iembq

Telephone: +86-29-3374552 Fax: +86-29-3374552

Li Liu, Zhi-Peng Wang, Qi-Bing Mei, Si-Yuan Zhou, Department of Pharmacology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Yin Long, Jia-Yun Liu, Department of Traditional Chinese Medicine, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Chang-Tai Xu, Editorial Department of Journal of Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Bo-Rong Pan, Department of Oncology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Qi-Bing Mei, Department of Pharmacology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. moc.liamtoh@iembq

Telephone: +86-29-3374552 Fax: +86-29-3374552

Received 2003 Mar 10; Revised 2003 Jun 12; Accepted 2003 Jun 19.

Abstract

AIM: To study the effects of Rheum tanguticum polysaccharide_-1 (RTP_-1) on ulcerative colitis in rats induced by 2, 4, 6-trinitrophene sulphonic acid (TNBS) and their possible mechanism.

METHODS: RTP₁ (200 mg·kg^{, ig) extracted from}Rheum tanguticum Maxim. ex Regel was administrated to rats with colitis induced by TNBS for 5 d, 7 d, 10 d and 14 d, respectively. The effects of RTP₁ and dexamethasone (DX, 0.2 mg·kg^{, ig) were contrastively investigated. The MPO level and SOD activity were determined by chromatometry. The expansion and protein expression of CD4}^{T lymphocytes isolated from colon mucosae and mesenteric lymph nodes of colitis rats were performed by immunohistochemical analysis and Western-blot methods.}

RESULTS: Treatments of RTP₁ (200 mg·kg^{, ig) significantly reduced diarrhea, mortality, colon mass, ulcer areas and MPO level in colon mucosae on days 5, 7, 10 and 14 (5.2 ± 1.4, 5.4 ± 0.7, 5.2 ± 1.8,}P < 0.05. 3.4 ± 0.8, P < 0.01. 16.1 ± 12.1, P < 0.01. 31.8 ± 8.6, 17.7 ± 5.3, 12.7 ± 4.1, P < 0.05). The effects of RTP₁ were similar to those noted above in DX group, but there were no immunosupressive effects of DX in RTP_-1 group, such as body mass loss, thymus and spleen atrophy. The decreased number and down-regulated protein levels of CD4^{T cells isolated from the colon of colitis rats treated with RTP}₁ were found.

CONCLUSION: RTP₁ shows significantly protective effects but lower side effects on rats with colitis induced by TNBS. The mechanism may be due to the resistance to over expansion of CD4.

Abstract

^P < 0.05,

Footnotes

Supported by the National Natural Science Foundation of China, No 30100239

Edited by Zhu L and Wang XL

Footnotes

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