Effect of Antioxidants, Amino Acids and Plant Growth Regulators on <em> in vitro</em> Propagation of <em>Rosa centifolia</em>

Gulzar Akhtar

Muhammad Jaskani

Yasar Sajjad

Ahsan Akram

^{Department of Horticulture, University College of Agriculture, University of Sargodha, Sargodha, Pakistan}

^{Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan}

^{Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad, Pakistan}

^{Corresponding author:} Gulzar Akhtar, Department of Horticulture, University College of Agriculture, University of Sargodha, Sargodha, Pakistan. Tel: +92-3216211912, Fax: +92-483703665, moc.oohay@9481ttub_razluG

Received 2015 Feb 8; Revised 2015 Jul 7; Accepted 2015 Dec 12.

Abstract

Background

Rosa centifoliais commercially propagated by asexual means but in vitro propagation ensure the production of disease free and healthy plants and browning of explants creates hurdle in their multiplication.

Objectives

The aim was to reduce oxidative browning of shoots of R. centifolia in MS medium during in vitro propagation.

Materials and Methods

Axillary buds of R. centifolia were sterilized with 70% ethyl alcohol for 4 min and 5% sodium hypochlorite for 2 min followed by three washing with sterilized double distilled water. In order to control oxidative browning, Ascorbic acid (100 mg.L^{), citric acid (100 mg.L}^{) and activated charcoal (3 g.L}^{) were used while to control withering of shoots, different concentrations (3.0 mg.L}^{, 6.0 mg.L}^{, 9.0 mg.L}^{) of either glutamine, asparagine and proline were put into trial. Different concentrations of Benzyl aminopurine (BAP) and naphthalene acetic acid (NAA) were used for}in vitro shoot and root formation.

Results

Minimum browning percentage (20%) was achieved in the presence of activated charcoal (3.0 g.L^{) and pretreatment of explants with running tap water. Asparagin (9.0 mg.L}^{) produced maximum shooting (93%), minimum withering (6.67%), and it took longer period (27 days) for shoots to wither. BAP (3.0 mg.L}^{) + NAA (0.5 mg.L}^{) was produced the highest number of shoots (1.63), in a shortest periods (9 days). For root production, NAA (1.5 mg.L}^{) + BAP (0.5 mg.L}^{) reduced the time to 11 days with maximum number of roots (4.33) and root length (4.20 cm).}

Conclusions

The supplement of activated charcoal (3.0 g.L^{), a sparagin (9.0 mg.L}^{) and combination of BAP and NAA in the MS medium is effective for}in vitro propagation of R. centifolia.

Keywords: Activated charcoal, Browning, In vitro Propagation , centifolia

Abstract

Effect of different treatments on browning of the MS medium

A: Browning of axillary buds in control MS medium, B: Browning of axillary buds in 3 g.L^{activated charcoal, C: Withering of}in vitro shoot in control, D: Withering of in vitro shoot in 0.09 mg.L^{Asparagin, E:}In vitro Shoot formation, F: In vitro Root induction, G: Acclimatization

Means with same letters are considered as non-significant

Acknowledgments

The present study was funded by the Higher Education Commission of Pakistan under indigenous fellowship program.

Acknowledgments

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