Abstract:

Electron probe microanalysis for K and Cl and enzymic determination of malate were performed on epidermal strips of Vicia faba L. which had been incubated with 0.1 equivalent of K(+) per liter in the absence or presence of Cl(-). In the absence of Cl(-), iminodiacetate, a presumed impermeant zwitterion, served as anion. With no Cl(-) in the medium, 91% of the K(+) imported into the guard cells during stomatal opening was neutralized by malate production; import of Cl(-) (presumably from the rest of the epidermal tissue) contributed 6%. In the presence of Cl(-), 50% of the necessary negative charges were provided by malate synthesis, 45% by Cl(-) import. Stomatal opening was not obviously affected by the chloride concentration in the incubation medium, but malate production declined roughly linearly with the logarithm of [Cl(-)] between 10(-5) and 10(-1) equivalent per liter.

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Availability of Chloride Affects the Balance between Potassium Chloride and Potassium Malate in Guard Cells of <em>Vicia faba</em> L. <sup><a href="#fn1" rid="fn1" class=" fn">1</a></sup>

K Raschke

H Schnabl

Abstract

Electron probe microanalysis for K and Cl and enzymic determination of malate were performed on epidermal strips of Vicia faba L. which had been incubated with 0.1 equivalent of K^{per liter in the absence or presence of Cl}^{. In the absence of Cl}^{, iminodiacetate, a presumed impermeant zwitterion, served as anion. With no Cl}^{in the medium, 91% of the K}^{imported into the guard cells during stomatal opening was neutralized by malate production; import of Cl}^{(presumably from the rest of the epidermal tissue) contributed 6%. In the presence of Cl}^{, 50% of the necessary negative charges were provided by malate synthesis, 45% by Cl}^{import. Stomatal opening was not obviously affected by the chloride concentration in the incubation medium, but malate production declined roughly linearly with the logarithm of [Cl}^{] between 10}^{and 10}^{equivalent per liter.}

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Selected References

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Humble GD, Raschke K. Stomatal opening quantitatively related to potassium transport: evidence from electron probe analysis. Plant Physiol. 1971 Oct;48(4):447–453.[PMC free article] [PubMed] [Google Scholar]
Jackson AO, Larkins BA. Influence of Ionic Strength, pH, and Chelation of Divalent Metals on Isolation of Polyribosomes from Tobacco Leaves. Plant Physiol. 1976 Jan;57(1):5–10.[PMC free article] [PubMed] [Google Scholar]
Van Kirk CA, Raschke K. Presence of Chloride Reduces Malate Production in Epidermis during Stomatal Opening. Plant Physiol. 1978 Mar;61(3):361–364.[PMC free article] [PubMed] [Google Scholar]

MSU-ERDA Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824

^{Permanent address: Institut für Botanik und Mikrobiologie der Technischen Universität München, Arcisstr. 21, D-8000 München 2, Federal Republic of Germany.}

^{Research supported by the U.S. Energy Research and Development Administration under Contract EY-76-C-02-1338.}

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Abstract

Electron probe microanalysis for K and Cl and enzymic determination of malate were performed on epidermal strips of Vicia faba L. which had been incubated with 0.1 equivalent of K^{per liter in the absence or presence of Cl}^{. In the absence of Cl}^{, iminodiacetate, a presumed impermeant zwitterion, served as anion. With no Cl}^{in the medium, 91% of the K}^{imported into the guard cells during stomatal opening was neutralized by malate production; import of Cl}^{(presumably from the rest of the epidermal tissue) contributed 6%. In the presence of Cl}^{, 50% of the necessary negative charges were provided by malate synthesis, 45% by Cl}^{import. Stomatal opening was not obviously affected by the chloride concentration in the incubation medium, but malate production declined roughly linearly with the logarithm of [Cl}^{] between 10}^{and 10}^{equivalent per liter.}

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