Rosa canina L. Fruit Hydro-Alcoholic Extract Effects on Some Immunological and Biochemical Parameters in Rats.
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Journal: 2013/May - BioImpacts
ISSN: 2228-5652
Abstract:
BACKGROUND
This research investigates the possible potential of Rosa canina (RC) as an immunomodulator in rats and its effects on some biochemical parameters.
METHODS
In this experiment, 45 male Wistar rats were obtained and divided into three groups (n = 15). These groups received normal saline (10 mg/kg), RC fruit extract (250 mg/kg) and RC fruit extract (500 mg/kg) as oral gavages every day for a period of four weeks, respective-ly. After obtaining blood samples (at the end of each week), differential white blood cell (WBC) counts, phagocyte activity (number), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphates (ALP) albumin and globulins levels of sam-ples were obtained. The malondialdehyde (MDA) and glutathione (GSH) levels in the se-rum were determined only in day 28 of study. The radical scavenger activity (RSA) of the RC extract was measured spectrophotometrically.
RESULTS
the gamma globulin level, neu-trophil and monocyte counts and phagocyte activity increased significantly in comparison with the normal saline group. ALT, AST and ALP had not significantly differences in compared to control group. RC extract significantly increased thiobarbituric acid reactive substances (TBARS) and also decreased GSH levels in comparing to control group in day 28.
CONCLUSIONS
the data suggest that the RC extract has been used in traditional medicine might have immunomodulatory effects.
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Bioimpacts 1(4): 219-224
PMID: 23678431

<em>Rosa canina L</em>. Fruit Hydro-Alcoholic Extract Effects on Some Immunological and Biochemical Parameters in Rats

Department of Molecular Neurobiology, Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Biochemistry, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Department of chemistry, Tabriz Branch, Payame Noor University, Tabriz, Iran
Department of Microbiology, Faculty of Science, Islamic Azad University of Lahijan, Lahijan, Iran
Department of Biotechnology, Faculty of Agriculture, Payame Noor University of Tehran, Karaj, Iran
Department Animal Science, Faculty of Agriculture, Islamic Azad University, Branch of Shabestar, Shabestar, Iran
Corresponding author: Saeed Sadigh-Eteghad (PhD), Tel.: +98 (914) 1038689,moc.liamg@dagetegidas.deeas
Received 2011 Sep 20; Revised 2011 Nov 10; Accepted 2011 Dec 8.
Copyright © 2011 by Tabriz University of Medical Sciences

Abstract

Introduction

This research investigates the possible potential of Rosa canina (RC) as an immunomodulator in rats and its effects on some biochemical parameters.

Methods

In this experiment, 45 male Wistar rats were obtained and divided into three groups (n = 15). These groups received normal saline (10 mg/kg), RC fruit extract (250 mg/kg) and RC fruit extract (500 mg/kg) as oral gavages every day for a period of four weeks, respective-ly. After obtaining blood samples (at the end of each week), differential white blood cell (WBC) counts, phagocyte activity (number), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphates (ALP) albumin and globulins levels of sam-ples were obtained. The malondialdehyde (MDA) and glutathione (GSH) levels in the se-rum were determined only in day 28 of study. The radical scavenger activity (RSA) of the RC extract was measured spectrophotometrically.

Results

the gamma globulin level, neu-trophil and monocyte counts and phagocyte activity increased significantly in comparison with the normal saline group. ALT, AST and ALP had not significantly differences in compared to control group. RC extract significantly increased thiobarbituric acid reactive substances (TBARS) and also decreased GSH levels in comparing to control group in day 28.

Conclusion

the data suggest that the RC extract has been used in traditional medicine might have immunomodulatory effects.

Keywords: Rosa canina, Immunological Parameters, Biochemical Parameters, Rats
Abstract

Differential WBCs percentage in various groups and days. Data is expressed as mean, bars represent standard deviation. *p<0.05 significant from normal saline.

Phagositic activity in various groups and days. Data is expressed as mean, bars represent standard deviation. *p<0.05 significant from normal saline.

Percentage of albumin and globulins percentage in various groups and days. Data is expressed as mean, bars represent standard deviation. *p<0.05 significant from normal saline.

normal saline group, herb 250 mg/kg, herb 500 mg/kg

Unit of used extract and vitamin C were (mg/ml) and (µg/ml) respectively.

TRABS and GSH levels in serum of experimental and control groups in day 28. *p<0.05 significant from normal saline.

Acknowledgments

The authors are thankful to research office of Tabriz Medical University for spiritual auspices.

Acknowledgments

References

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