Comparative Study on the Effect of Plantago psyllium and Ocimum basilicum Seeds on Anthropometric Measures in Nonalcoholic Fatty Liver Patients

INTRODUCTION

Nonalcoholic fatty liver disease (NAFLD) as a silent killer with accumulating extra fat in hepatocytes more than 5% with no history of alcohol abuse, follows by creating inflammation, nonalcoholic steatohepatitis, which leads to liver fibrosis and finally liver cirrhosis.[1] High prevalence of NAFLD in diabetic patients and obese individuals showed the relationship between NAFLD and these chronic diseases which insulin resistance considered as the main reason.[2] Due to 25%–30% prevalence of total population,[3] finding solutions for NAFLD has an exclusive importance in public health.

Even though life styles modifications such as weight lose[4] are suggested, nutritional patterns changes are considered as important approaches in NAFLD management.[5] Simple carbohydrates, especially fructose and sucrose cause fat accumulation in body tissues such as liver.[6] Many studies assessed effect of nutrients such as Vitamin D, symbiotic and green tea as a solution.[789] Moreover, most NAFLD patients daily consume much lower fiber than required amounts.[10]

Plantago psyllium (PP) and Ocimum basilicum (OB) as two types of high fiber and mucilage (10%–15%),[11] plant seeds can consider as the key solution of NAFLD by decreasing appetite with occupying stomach and decreasing food glysemic index.

METHODS

Intervention and monitoring

We supplied PP and OB seeds from the center of Iran, Isfahan, which packaged in 2 5 g parts for daily (10 g) usage. Each pack should be eaten 20–30 min before lunch and dinner with only a glass of water and no additional sugar or even artificial sweeteners for 3 months. We followed patient with weekly texting and monthly calling.

Mucilage of PP and OB absorb water and get a sticky and viscose condition which leads to unpleasant feeling, so we lost participants and mentioned in Figure 1.

Figure 1

Flow diagram of the progress through the phases of study

RESULTS

Eight participants could not tolerate PP and OB because of constipation, nausea or stomach-ache and were excluded from our study. Moreover, 42 patients that were involved to exclusion criteria during the study or they did not want to continue were eliminated. There were no significant differences regarding sex distribution, smoking condition, education status, and body weight in four groups at baseline. However, age was the only factor that was significantly different among groups which was adjusted in our analysis. No significant difference of receiving pill was observed among groups. Table 1 is showing all participants demographic information.

Table 1

Baseline demographic information of participants

First of all, we compared before and after anthropometric measurements in different groups separately that some of them had been significantly decreased. Weight (P = 0.02), BMI (P = 0.01), and MBF (P = 0.04) in OB group were significantly reduced. Moreover, weight (P = 0.00), BMI, SLM, and lean body mass (LBM) (P = 0.01) were lowered in PP group. In PP and OB mixed group, we saw a similar result to the last group. Weight, BMI, SLM, and LBM were significantly different while, SLM of trunk was marginally significant. Similarly, control group data showed considerable reduction in weight and BMI P = 0.00 and 0.02, respectively, we examined among group changes as more important aim in order to reach reliable consequences of our intervention that we did not see any significant effects among groups [Table 2].

Table 2

Result of within group and between groups comparison

In addition, we averaged out a 3-day food and a 2-day record in order to controlled confounding such as a total dietary, total fiber, insoluble fiber, and soluble fiber intake [Table 3] and also, physical activity [Table 1]. None of the mean dietary confounding factors and mean of physical activity was significantly different among four groups.

Table 3

Baseline dietary intakes

DISCUSSION

This study illustrates a 10 g increasing of PP, OB, or mix of them have no effects on weight, BMI, % PBF, or SLM during 12 weeks. Previous studies have recommended that increased fiber intake up to Recommended Dietary Allowance (RDA) amount (38 for men and 25 g for women[17] leads to increased postmeal satiety.[18] Moreover, the gut hormone, ghrelin, gastrointestinal peptide, and glucagon-like peptide (GLP-1) were increased. Ghrelin can suppress appetite and GLP-1 can enhance postprandial satiety time.[19]

However, the effects of fiber on weight loss are controversial. Similar to our study, no protective effect of PP on weight change did not observed in 36 diabetic patients.[20] Likewise, when soluble fiber was compared against insoluble fiber neither of them have cause reduction in weight.[21]

In contrast, some documents showed positive outcomes of soluble fiber in anthropometric characters. High dose of PP (36 g daily) demonstrated considerable decrease in weight, BMI, and, especially % body fat either with a diet modification or without that.[22] Similarly, both soluble (24 g PP) and insoluble (28 g wheat bran) elucidated a significantly weight loss after 6 weeks, while the participants had gone on a modified diet.[23]

As other studies, our study includes some limitations inevitably. Some used drugs by patients to improve liver status, might fade the effects of PP and OB on anthropometric measures. Our participants were new cases of NAFLD who may have gone on a diet unconsciously.

The aim of present study was to demonstrate protective effect of PP and OB mixture on weight reduction but it was not observed which can be due to the low-dose of soluble fiber used in present study.

In a similar meta-analysis, the effect of guar gum on body weight was evaluated rigorously. As a consequence, when 11 randomized controlled trials were put together, no significant robust effect of guar gum consumption on weight loss was observed.[24] Although most animal studies showed a significant effect of fiber on weight reduction,[25] human studies have yielded inconsistent inferences.[2021] Likewise, it is shown in another systematic review that soluble fiber has no effect on weight loss.[26] However, it should be noted in our study and other similar studies, participant's baseline weight was normal or overweight which may have precluded the expected results. This study has been registered both in Iranian registry clinical trial (IRCT2014101811763N17) and Isfahan University of Medical Sciences (393583).

CONCLUSIONS

This study indicated that supplementation with 10 mg/day PP, 10 mg/day OB, and 10 mg/day PP and OB mixed for 12 weeks did not improve weight, BMI, SLM, LBM, MBF, and PBF significantly. Further trials should be done in this area with higher dose of PP and OB and also, in bigger population which draw a clear link between anthropometric measures and fiber supplementation.

This study was funded by Isfahan University of Medical Sciences and Sedigheh Tahereh, Isfahan Endocrine and Metabolism Research Center was involved in data collection.