Abstract:

Detached pistils of Lilium longiflorum were labeled with d-glucose-U-(14)C 24 hours after anthesis and then sampled for the next 6 days to determine the appearance of label into exudate from the stylar canal and the stigmatic surface of the pistil. Results were obtained with unpollinated cv. Ace pistils and pollinated cv. Ace pistils, selfed or crossed (cv. Croft pollen). Limited data were also obtained on cv. Croft pistils, selfed or crossed (cv. Ace pollen).Exudate appeared in the canal and on the stigmatic surface soon after anthesis. In unpollinated pistils it continued to accumulate for about 5 days in the canal and for the full term of the experiment, 7 days, on the stigmatic surface. Canal exudate eventually mixed with stigmatic exudate in unpollinated pistils. Pollination interrupted the flow of exudate, and a portion of the pistil secretion product was diverted toward the synthetic requirements of the developing pollen tubes. Two days after pollination, the specific radioactivity of pollen tube cytoplasm had reached a level comparable to canal exudate. Twenty-four hours later, the specific radioactivity of pollen tube wall substance was about 80% of the value found in canal exudate. Similar patterns of (14)C incorporation and similar carbohydrate contents were obtained from self- and cross-pollinated pistils, although the former contained pollen tubes of less than one-half the length of the latter.

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The Nutritional Role of Pistil Exudate in Pollen Tube Wall Formation in <em>Lilium longiflorum</em>

C Labarca

F Loewus

Abstract

Detached pistils of Lilium longiflorum were labeled with d-glucose-U-^{C 24 hours after anthesis and then sampled for the next 6 days to determine the appearance of label into exudate from the stylar canal and the stigmatic surface of the pistil. Results were obtained with unpollinated cv. Ace pistils and pollinated cv. Ace pistils, selfed or crossed (cv. Croft pollen). Limited data were also obtained on cv. Croft pistils, selfed or crossed (cv. Ace pollen).}

Exudate appeared in the canal and on the stigmatic surface soon after anthesis. In unpollinated pistils it continued to accumulate for about 5 days in the canal and for the full term of the experiment, 7 days, on the stigmatic surface. Canal exudate eventually mixed with stigmatic exudate in unpollinated pistils. Pollination interrupted the flow of exudate, and a portion of the pistil secretion product was diverted toward the synthetic requirements of the developing pollen tubes. Two days after pollination, the specific radioactivity of pollen tube cytoplasm had reached a level comparable to canal exudate. Twenty-four hours later, the specific radioactivity of pollen tube wall substance was about 80% of the value found in canal exudate. Similar patterns of ^{C incorporation and similar carbohydrate contents were obtained from self- and cross-pollinated pistils, although the former contained pollen tubes of less than one-half the length of the latter.}

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Kroh M, Miki-Hirosige H, Rosen W, Loewus F. Incorporation of label into pollen tube walls from myoinositol-labeled Lilium longiflorum pistils. Plant Physiol. 1970 Jan;45(1):92–94.[PMC free article] [PubMed] [Google Scholar]
Labarca C, Kroh M, Loewus F. The Composition of Stigmatic Exudate from Lilium longiflorum: Labeling Studies with Myo-inositol, d-Glucose, and l-Proline. Plant Physiol. 1970 Jul;46(1):150–156.[PMC free article] [PubMed] [Google Scholar]
Labarca C, Loewus F. The Nutritional Role of Pistil Exudate in Pollen Tube Wall Formation in Lilium longiflorum: I. Utilization of Injected Stigmatic Exudate. Plant Physiol. 1972 Jul;50(1):7–14.[PMC free article] [PubMed] [Google Scholar]
Hubbert WT, Rosen MN. Pasteurella multocida Infections: I. Pasteurella Multocida Infection Due to Animal Bite. Am J Public Health Nations Health. 1970 Jun;60(6):1103–1108.[PMC free article] [PubMed] [Google Scholar]

^{Department of Biology, State University of New York at Buffalo, Buffalo, New York 14214}

^{Present address: Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile.}

^{This investigation was supported by National Institutes of Health Research Grant GM-12422 from the National Institute of General Medical Sciences. A preliminary report was presented at a symposium on the “Biogenesis of Plant Cell Wall Polysaccharides,” 164th National Meeting, American Chemical Society, New York (6).}

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Abstract

Detached pistils of Lilium longiflorum were labeled with d-glucose-U-^{C 24 hours after anthesis and then sampled for the next 6 days to determine the appearance of label into exudate from the stylar canal and the stigmatic surface of the pistil. Results were obtained with unpollinated cv. Ace pistils and pollinated cv. Ace pistils, selfed or crossed (cv. Croft pollen). Limited data were also obtained on cv. Croft pistils, selfed or crossed (cv. Ace pollen).}Exudate appeared in the canal and on the stigmatic surface soon after anthesis. In unpollinated pistils it continued to accumulate for about 5 days in the canal and for the full term of the experiment, 7 days, on the stigmatic surface. Canal exudate eventually mixed with stigmatic exudate in unpollinated pistils. Pollination interrupted the flow of exudate, and a portion of the pistil secretion product was diverted toward the synthetic requirements of the developing pollen tubes. Two days after pollination, the specific radioactivity of pollen tube cytoplasm had reached a level comparable to canal exudate. Twenty-four hours later, the specific radioactivity of pollen tube wall substance was about 80% of the value found in canal exudate. Similar patterns of ^{C incorporation and similar carbohydrate contents were obtained from self- and cross-pollinated pistils, although the former contained pollen tubes of less than one-half the length of the latter.}

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