The main object of the present study is to investigate genotypic differences in strontium (Sr) resistance of two oat varieties, including Neimengkeyi-1 (high accumulator) and Bayou-3 (low accumulator). The growth and antioxidant enzyme responses to five Sr concentrations (0, 25, 100, 500, and 1000 mg kg^-1) were compared after 30-day Sr treatment. The shoot-Sr concentrations of Neimengkeyi-1 were higher than those of Bayou-3 in all treatments. The translocation factors of Neimengkeyi-1 in all treatments were greater than 1 and significantly higher than those of Bayou-3. Sr ions did not affect the growth of Neimengkeyi-1. The H₂O₂ contents and the leaf malondialdehyde contents of Neimengkeyi-1 were lower than those of Bayou-3. The activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase of Neimengkeyi-1 were significantly higher than those of Bayou-3. Significant negative correlations were found between H₂O₂ contents and SOD and CAT activities in Neimengkeyi-1. The results of this study suggest that Sr accumulation patterns have significant genotypic differences, and SOD and CAT may play a pivotal role in the detoxification mechanism of Sr.