Abstract:

Leaf epidermal strips of Vicia faba were floated on 10 millimolar KCl at temperatures of 10 to 45 C for several time periods in the dark. For the duration of the experiment, a jet of water-saturated, CO(2)-free air was passed over the strips. The width of the stomatal aperture was measured microscopically and the potassium content was estimated and expressed as the percentage of area of the guard cell pair stained with cobaltinitrite. Stomatal opening in the dark occurred at temperatures above 35 C. The high temperature aperture reduction previously observed at 45 C in the light was not observed in the dark. Potassium accumulation occurred at all temperatures except 10 C, with a maximum at 45 C, which appeared functionally different from that seen previously in the light.

Open in

PUBMED | PMC | Google Scholar | Wikipedia

Relations:

Content

Citations

(1)

References

(6)

Affiliates

(1)

Dark Opening of Stomates of <em>Vicia faba</em> in CO<sub>2</sub>-free Air

C Rogers

Abstract

Leaf epidermal strips of Vicia faba were floated on 10 millimolar KCl at temperatures of 10 to 45 C for several time periods in the dark. For the duration of the experiment, a jet of water-saturated, CO₂-free air was passed over the strips. The width of the stomatal aperture was measured microscopically and the potassium content was estimated and expressed as the percentage of area of the guard cell pair stained with cobaltinitrite. Stomatal opening in the dark occurred at temperatures above 35 C. The high temperature aperture reduction previously observed at 45 C in the light was not observed in the dark. Potassium accumulation occurred at all temperatures except 10 C, with a maximum at 45 C, which appeared functionally different from that seen previously in the light.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (502K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

Fischer RA. Stomatal Opening in Isolated Epidermal Strips of Vicia faba. I. Response to Light and to CO(2)-free Air. Plant Physiol. 1968 Dec;43(12):1947–1952.[PMC free article] [PubMed] [Google Scholar]
Fischer RA. Role of Potassium in Stomatal Opening in the Leaf of Vicia faba. Plant Physiol. 1971 Apr;47(4):555–558.[PMC free article] [PubMed] [Google Scholar]
Fischer RA, Hsiao TC. Stomatal Opening in Isolated Epidermal Strips of Vicia faba. II. Responses to KCl Concentration and the Role of Potassium Absorption. Plant Physiol. 1968 Dec;43(12):1953–1958.[PMC free article] [PubMed] [Google Scholar]
Humble GD, Hsiao TC. Specific requirement of potassium for light-activated opening of stomata in epidermal strips. Plant Physiol. 1969 Feb;44(2):230–234.[PMC free article] [PubMed] [Google Scholar]
Rogers CA. Relationship of Temperature to Stomatal Aperture and Potassium Accumulation in Guard Cells of Vicia faba. Plant Physiol. 1979 Feb;63(2):388–391.[PMC free article] [PubMed] [Google Scholar]
Willmer CM, Pallas JE, Black CC. Carbon dioxide metabolism in leaf epidermal tissue. Plant Physiol. 1973 Nov;52(5):448–452.[PMC free article] [PubMed] [Google Scholar]

^{Biosystems Research Division, Bioengineering Program.}

^{Department of Plant Sciences, Texas Agricultural Experiment Station.}

^{This work was supported by the Division of Environmental Biology of the National Science Foundation Grant DEB 77/14408 to the Biosystems Research Division, Department of Industrial Engineering and by the Texas Agricultural Experiment Station, Texas A&M University.}

Copyright notice

Abstract

Leaf epidermal strips of Vicia faba were floated on 10 millimolar KCl at temperatures of 10 to 45 C for several time periods in the dark. For the duration of the experiment, a jet of water-saturated, CO₂-free air was passed over the strips. The width of the stomatal aperture was measured microscopically and the potassium content was estimated and expressed as the percentage of area of the guard cell pair stained with cobaltinitrite. Stomatal opening in the dark occurred at temperatures above 35 C. The high temperature aperture reduction previously observed at 45 C in the light was not observed in the dark. Potassium accumulation occurred at all temperatures except 10 C, with a maximum at 45 C, which appeared functionally different from that seen previously in the light.

Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.

Learn More