Primary afferent depolarization of cat pudendal afferents during micturition and segmental afferent stimulation.
PDF (1.9M)
Journal: 1995/February - Journal of Physiology
ISSN: 0022-3751
PUBMED: 7837101
Abstract:
1. This investigation examined primary afferent depolarization (PAD) of perineal afferents during micturition and evoked by electrical stimulation of perineal, hindlimb cutaneous and muscle-nerves. PAD was inferred from changes in excitability of spinal terminals of single afferents in decerebrate and chloralose-anaesthetized paralysed male cats. Observations were made on perineal afferent fibres travelling in the sensory branch of the pudendal (SPud) and superficial perineal (SPeri) nerves. 2. Micturition was evoked by distension of the bladder and excitability changes were measured in twenty-seven SPud afferents. In ten afferents, there was evidence of PAD during micturition. The time course of PAD was similar to the period of decreased activity in sphincter muscle efferents during micturition. In four afferents, there was decreased excitability during voiding that was interpreted as removal of tonic PAD. In the remaining thirteen afferents there were no detectable changes in excitability. Bladder distension in the absence of micturition failed to change the excitability of any SPud afferents tested. 3. Almost all SPud afferents were subject to PAD upon stimulation of cutaneous nerves. Superficial perineal, long saphenous, caudal cutaneous sural and the predominantly cutaneous posterior tibial nerves were particularly effective in evoking PAD. While group I strength stimulation of hindlimb muscle-nerves produced PAD of some SPud fibres, group II stimulation often increased the magnitude or incidence of PAD. The patterns and magnitude of PAD observed in SPeri afferents were similar to those observed in SPud afferents. 4. Since some SPud afferents were subject to PAD during micturition, PAD is probably one mechanism responsible for suppression of sphincter reflexes during micturition. Additional roles of PAD of perineal afferents evoked by activation of hindlimb cutaneous and muscle afferents are discussed.
Open in
PUBMED | PMC | Google Scholar | Wikipedia
Relations:
Content
Citations
(10)
References
(34)
Conditions
(1)
Organisms
(2)
Processes
(3)
Anatomy
(5)
Affiliates
(1)
Similar articles
Articles by the same authors
Discussion board
J Physiol 479(Pt 3): 451-461
PMID: 7837101

Primary afferent depolarization of cat pudendal afferents during micturition and segmental afferent stimulation.

Abstract

1. This investigation examined primary afferent depolarization (PAD) of perineal afferents during micturition and evoked by electrical stimulation of perineal, hindlimb cutaneous and muscle-nerves. PAD was inferred from changes in excitability of spinal terminals of single afferents in decerebrate and chloralose-anaesthetized paralysed male cats. Observations were made on perineal afferent fibres travelling in the sensory branch of the pudendal (SPud) and superficial perineal (SPeri) nerves. 2. Micturition was evoked by distension of the bladder and excitability changes were measured in twenty-seven SPud afferents. In ten afferents, there was evidence of PAD during micturition. The time course of PAD was similar to the period of decreased activity in sphincter muscle efferents during micturition. In four afferents, there was decreased excitability during voiding that was interpreted as removal of tonic PAD. In the remaining thirteen afferents there were no detectable changes in excitability. Bladder distension in the absence of micturition failed to change the excitability of any SPud afferents tested. 3. Almost all SPud afferents were subject to PAD upon stimulation of cutaneous nerves. Superficial perineal, long saphenous, caudal cutaneous sural and the predominantly cutaneous posterior tibial nerves were particularly effective in evoking PAD. While group I strength stimulation of hindlimb muscle-nerves produced PAD of some SPud fibres, group II stimulation often increased the magnitude or incidence of PAD. The patterns and magnitude of PAD observed in SPeri afferents were similar to those observed in SPud afferents. 4. Since some SPud afferents were subject to PAD during micturition, PAD is probably one mechanism responsible for suppression of sphincter reflexes during micturition. Additional roles of PAD of perineal afferents evoked by activation of hindlimb cutaneous and muscle afferents are discussed.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
icon of scanned page 451
451
icon of scanned page 452
452
icon of scanned page 453
453
icon of scanned page 454
454
icon of scanned page 455
455
icon of scanned page 456
456
icon of scanned page 457
457
icon of scanned page 458
458
icon of scanned page 459
459
icon of scanned page 460
460
icon of scanned page 461
461

Images in this article

Figure 2
Figure 2
on p.454
Figure 8
Figure 8
on p.460
Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bayev KV, Kostyuk PG. Primary afferent depolarization evoked by the activity of spinal scratching generator. Neuroscience. 1981;6(2):205–215. [PubMed] [Google Scholar]
  • BISHOP B. Reflex activity of external anal sphincter of cat. J Neurophysiol. 1959 Nov;22:679–692. [PubMed] [Google Scholar]
  • Brink E, Jankowska E, Skoog B. Convergence onto interneurons subserving primary afferent depolarization of group I afferents. J Neurophysiol. 1984 Mar;51(3):432–449. [PubMed] [Google Scholar]
  • Cottrell DF, Iggo A, Kitchell RL. Electrophysiology of the afferent innervation of the penis of the domestic ram. J Physiol. 1978 Oct;283:347–367.[PMC free article] [PubMed] [Google Scholar]
  • Curtis DR, Lodge D. The depolarization of feline ventral horn group Ia spinal afferent terminations by GABA. Exp Brain Res. 1982;46(2):215–233. [PubMed] [Google Scholar]
  • Decima EE, Goldberg LJ. Centrifugal dorsal root discharges induced by motoneurone activation. J Physiol. 1970 Mar;207(1):103–118.[PMC free article] [PubMed] [Google Scholar]
  • Dueñas SH, Rudomin P. Excitability changes of ankle extensor group Ia and Ib fibers during fictive locomotion in the cat. Exp Brain Res. 1988;70(1):15–25. [PubMed] [Google Scholar]
  • Edgley SA, Jankowska E. Field potentials generated by group II muscle afferents in the middle lumbar segments of the cat spinal cord. J Physiol. 1987 Apr;385:393–413.[PMC free article] [PubMed] [Google Scholar]
  • Fedirchuk B, Hochman S, Shefchyk SJ. An intracellular study of perineal and hindlimb afferent inputs onto sphincter motoneurons in the decerebrate cat. Exp Brain Res. 1992;89(3):511–516. [PubMed] [Google Scholar]
  • Fedirchuk B, Shefchyk SJ. Membrane potential changes in sphincter motoneurons during micturition in the decerebrate cat. J Neurosci. 1993 Jul;13(7):3090–3094.[PMC free article] [PubMed] [Google Scholar]
  • Fedirchuk B, Song L, Downie JW, Shefchyk SJ. Spinal distribution of extracellular field potentials generated by electrical stimulation of pudendal and perineal afferents in the cat. Exp Brain Res. 1992;89(3):517–520. [PubMed] [Google Scholar]
  • GARRY RC, ROBERTS TD, TODD JK. Reflexes involving the external urethral sphincter in the cat. J Physiol. 1959 Dec;149:653–665.[PMC free article] [PubMed] [Google Scholar]
  • Gossard JP, Cabelguen JM, Rossignol S. Intra-axonal recordings of cutaneous primary afferents during fictive locomotion in the cat. J Neurophysiol. 1989 Nov;62(5):1177–1188. [PubMed] [Google Scholar]
  • Harrison PJ, Jankowska E. Primary afferent depolarization of central terminals of group II muscle afferents in the cat spinal cord. J Physiol. 1989 Apr;411:71–83.[PMC free article] [PubMed] [Google Scholar]
  • Jankowska E, McCrea D, Rudomín P, Sykova E. Observations on neuronal pathways subserving primary afferent depolarization. J Neurophysiol. 1981 Sep;46(3):506–516. [PubMed] [Google Scholar]
  • Jankowska E, Riddell JS. Interneurones in pathways from group II muscle afferents in sacral segments of the feline spinal cord. J Physiol. 1994 Mar 15;475(3):455–468.[PMC free article] [PubMed] [Google Scholar]
  • Jankowska E, Riddell JS, McCrea DA. Primary afferent depolarization of myelinated fibres in the joint and interosseous nerves of the cat. J Physiol. 1993 Jul;466:115–131.[PMC free article] [PubMed] [Google Scholar]
  • Johnson RD, Kitchell RL, Gilanpour H. Rapidly and slowly adapting mechanoreceptors in the glans penis of the cat. Physiol Behav. 1986;37(1):69–78. [PubMed] [Google Scholar]
  • Kruse MN, Mallory BS, Noto H, Roppolo JR, de Groat WC. Modulation of the spinobulbospinal micturition reflex pathway in cats. Am J Physiol. 1992 Mar;262(3 Pt 2):R478–R484. [PubMed] [Google Scholar]
  • Madrid J, Alvarado J, Dutton H, Rudomín P. A method for the dynamic continuous estimation of excitability changes of single fiber terminals in the central nervous system. Neurosci Lett. 1979 Mar;11(3):253–258. [PubMed] [Google Scholar]
  • Mendell L. Properties and distribution of peripherally evoked presynaptic hyperpolarization in cat lumbar spinal cord. J Physiol. 1972 Nov;226(3):769–792.[PMC free article] [PubMed] [Google Scholar]
  • Pearson KG, Rossignol S. Fictive motor patterns in chronic spinal cats. J Neurophysiol. 1991 Dec;66(6):1874–1887. [PubMed] [Google Scholar]
  • Rampal G, Mignard P. Organization of the nervous control of urethral sphincter. A study in the anaesthetized cat with intact central nervous system. Pflugers Arch. 1975;353(1):21–31. [PubMed] [Google Scholar]
  • Riddell JS, Jankowska E, Eide E. Depolarization of group II muscle afferents by stimuli applied in the locus coeruleus and raphe nuclei of the cat. J Physiol. 1993 Feb;461:723–741.[PMC free article] [PubMed] [Google Scholar]
  • Rudomín P, Engberg I, Jiménez I. Mechanisms involved in presynaptic depolarization of group I and rubrospinal fibers in cat spinal cord. J Neurophysiol. 1981 Sep;46(3):532–548. [PubMed] [Google Scholar]
  • Rudomin P, Nuñez R, Madrid J, Burke RE. Primary afferent hyperpolarization and presynaptic facilitation of Ia afferent terminals induced by large cutaneous fibers. J Neurophysiol. 1974 May;37(3):413–429. [PubMed] [Google Scholar]
  • Schmidt RF. Presynaptic inhibition in the vertebrate central nervous system. Ergeb Physiol. 1971;63:20–101. [PubMed] [Google Scholar]
  • Sethi RK, Bauer SB, Dyro FM, Krarup C. Modulation of the bulbocavernosus reflex during voiding: loss of inhibition in upper motor neuron lesions. Muscle Nerve. 1989 Nov;12(11):892–897. [PubMed] [Google Scholar]
  • Shefchyk SJ. The effects of lumbosacral deafferentation on pontine micturition centre-evoked voiding in the decerebrate cat. Neurosci Lett. 1989 Apr 24;99(1-2):175–180. [PubMed] [Google Scholar]
  • Shimoda N, Takakusaki K, Nishizawa O, Tsuchida S, Mori S. The changes in the activity of pudendal motoneurons in relation to reflex micturition evoked in decerebrate cats. Neurosci Lett. 1992 Feb 3;135(2):175–178. [PubMed] [Google Scholar]
  • Sundin T, Carlsson CA, Kock NG. Detrusor inhibition induced from mechanical stimulation of the anal region and from electrical stimulation of pudendal nerve afferents. An experimental study in cats. Invest Urol. 1974 Mar;11(5):374–378. [PubMed] [Google Scholar]
  • TODD JK. AFFERENT IMPULSES IN THE PUDENDAL NERVES OF THE CAT. Q J Exp Physiol Cogn Med Sci. 1964 Jul;49:258–267. [PubMed] [Google Scholar]
  • Wójcik G, Lupa K, Niechaj A. Differential decerebrate control of depolarization in the central terminals of cutaneous afferents in the sacral cord. Brain Res. 1983 May 5;266(2):233–241. [PubMed] [Google Scholar]
  • Woolf CJ, Fitzgerald M. Do opioid peptides mediate a presynaptic control of C-fibre transmission in the rat spinal cord? Neurosci Lett. 1982 Mar 17;29(1):67–72. [PubMed] [Google Scholar]
Department of Physiology, University of Manitoba, Winnipeg, Canada.
Department of Physiology, University of Manitoba, Winnipeg, Canada.
Copyright notice
Abstract
1. This investigation examined primary afferent depolarization (PAD) of perineal afferents during micturition and evoked by electrical stimulation of perineal, hindlimb cutaneous and muscle-nerves. PAD was inferred from changes in excitability of spinal terminals of single afferents in decerebrate and chloralose-anaesthetized paralysed male cats. Observations were made on perineal afferent fibres travelling in the sensory branch of the pudendal (SPud) and superficial perineal (SPeri) nerves. 2. Micturition was evoked by distension of the bladder and excitability changes were measured in twenty-seven SPud afferents. In ten afferents, there was evidence of PAD during micturition. The time course of PAD was similar to the period of decreased activity in sphincter muscle efferents during micturition. In four afferents, there was decreased excitability during voiding that was interpreted as removal of tonic PAD. In the remaining thirteen afferents there were no detectable changes in excitability. Bladder distension in the absence of micturition failed to change the excitability of any SPud afferents tested. 3. Almost all SPud afferents were subject to PAD upon stimulation of cutaneous nerves. Superficial perineal, long saphenous, caudal cutaneous sural and the predominantly cutaneous posterior tibial nerves were particularly effective in evoking PAD. While group I strength stimulation of hindlimb muscle-nerves produced PAD of some SPud fibres, group II stimulation often increased the magnitude or incidence of PAD. The patterns and magnitude of PAD observed in SPeri afferents were similar to those observed in SPud afferents. 4. Since some SPud afferents were subject to PAD during micturition, PAD is probably one mechanism responsible for suppression of sphincter reflexes during micturition. Additional roles of PAD of perineal afferents evoked by activation of hindlimb cutaneous and muscle afferents are discussed.
Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.