Immunochemical Analysis of the Temporal and Tissue-Specific Expression of an <em>Avena sativa</em> Plasma Membrane Determinant <sup><a href="#fn1" rid="fn1" class=" fn">1</a></sup>
Abstract
An immunoglobulin Mk monoclonal (F8IVE9) antibody raised against oat (Avena sativa cv Garry) root homogenate has been produced and characterized. The predominant target bound by this antibody is a 62-kilodalton protein (p62) that is expressed in both oat root and oat shoot cells. Treatment of the oat antigen with periodate, or with recombinant N-glycanase, affects the F8IVE9 binding to the antigen, suggesting that the specific epitope for this monoclonal antibody involves a carbohydrate determinant. Levels of p62 present in cells of the oat root increase approximately twofold as the root tissue matures during the first 11 days postgermination. In contrast, levels of expression in shoot tissue remain relatively constant during the same period. The p62 antigen has been shown to be expressed at the plasma membrane by immunohistochemical means, by immunofluorescent labeling of protoplasts, and by enzyme-linked immunosorbent assay analysis of purified plasma membrane. The F8IVE9 antigenic target appears to be uniformly distributed through root tissue but is differentially expressed in specific sections of the shoot. F8IVE9 antibody also binds to antigens expressed in a number of other species, including clover, corn, pea, broccoli, mustard, and bean, and has been shown to bind to Samanea protoplast plasma membranes. This monoclonal antibody may prove to be useful for a variety of investigations, including an analysis of the specific patterns of cellular differentiation that occur during early morphogenesis, and the characterization of plasma membrane-associated elements in plants.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barbier-Brygoo H, Ephritikhine G, Klämbt D, Ghislain M, Guern J. Functional evidence for an auxin receptor at the plasmalemma of tobacco mesophyll protoplasts. Proc Natl Acad Sci U S A. 1989 Feb;86(3):891–895.[PMC free article] [PubMed] [Google Scholar]
- Bush DR, Langston-Unkefer PJ. Amino Acid transport into membrane vesicles isolated from zucchini : evidence of a proton-amino Acid symport in the plasmalemma. Plant Physiol. 1988 Oct;88(2):487–490.[PMC free article] [PubMed] [Google Scholar]
- Chin JJ. Monoclonal antibodies that immunoreact with a cation-stimulated plant membrane ATPase. Biochem J. 1982 Apr 1;203(1):51–54.[PMC free article] [PubMed] [Google Scholar]
- de Boer AH, Watson BA, Cleland RE. Purification and identification of the fusicoccin binding protein from oat root plasma membrane. Plant Physiol. 1989;89:250–259.[PMC free article] [PubMed] [Google Scholar]
- Gorton HL, Satter RL. Extensor and flexor protoplasts from samanea pulvini : I. Isolation and initial characterization. Plant Physiol. 1984 Nov;76(3):680–684.[PMC free article] [PubMed] [Google Scholar]
- Heukeshoven J, Dernick R. Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels. Electrophoresis. 1988 Jan;9(1):28–32. [PubMed] [Google Scholar]
- Hussey PJ, Haas N, Hunsperger J, Larkin J, Snustad DP, Silflow CD. The beta-tubulin gene family in Zea mays: two differentially expressed beta-tubulin genes. Plant Mol Biol. 1990 Dec;15(6):957–972. [PubMed] [Google Scholar]
- Lamb CJ, Lawton MA, Dron M, Dixon RA. Signals and transduction mechanisms for activation of plant defenses against microbial attack. Cell. 1989 Jan 27;56(2):215–224. [PubMed] [Google Scholar]
- Lin CT, Sun D, Song GX, Wu JY. Calmodulin: localization in plant tissues. J Histochem Cytochem. 1986 May;34(5):561–567. [PubMed] [Google Scholar]
- Marshak-Rothstein A, Fink P, Gridley T, Raulet DH, Bevan MJ, Gefter ML. Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J Immunol. 1979 Jun;122(6):2491–2497. [PubMed] [Google Scholar]
- Rudofsky UH, Dilwith RL, Lynes M, Flaherty L. Murine monoclonal antikidney autoantibodies. I. Anti-renal proximal tubular basement membrane autoantibodies. Clin Immunol Immunopathol. 1982 Nov;25(2):165–178. [PubMed] [Google Scholar]
- Tate BF, Schaller GE, Sussman MR, Crain RC. Characterization of a Polyphosphoinositide Phospholipase C from the Plasma Membrane of Avena sativa. Plant Physiol. 1989 Dec;91(4):1275–1279.[PMC free article] [PubMed] [Google Scholar]
- Woodward MP, Young WW, Jr, Bloodgood RA. Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation. J Immunol Methods. 1985 Apr 8;78(1):143–153. [PubMed] [Google Scholar]