Background: Robotic liver surgery is a novel technique expanding the field of minimally invasive approaches. An increasing number of studies assess the outcomes of robotic liver resections (RLR). The aim of our meta-analysis is to provide an up-to-date comparison of RLR versus open liver resections (OLR), evaluating its safety and efficacy.

Materials and methods: A systematic search of MEDLINE, Scopus, Google Scholar, Cochrane, and Clinicaltrials.gov for articles published from January 2000 until January 2022 was undertaken.

Results: Thirteen non-randomized retrospective and one prospective clinical study enlisting 1801 patients met our inclusion criteria, with 640 patients undergoing RLR and 1161 undergoing OLR. RLR resulted in significantly lower overall morbidity (p < 0.001), shorter length of hospital stay (p = 0.002), and less intraoperative blood loss (p < 0.001). Operative time was found to be significantly higher in the RLR group (p < 0.001). Blood transfusion requirements, R0 resection, and mortality rates presented no difference among the two groups. The cumulative rate of conversion was 5% in the RLR group.

Conclusion: The increasing experience in the implementation of the robot will undoubtedly generate more prospective randomized studies, necessary to assess its potential superiority over the traditional open approach, in a variety of hepatic lesions.

Keywords: Hepatectomy; Liver resection; Robot-assisted; Robotic.

Disclosure of new molecular probes as chromogenic and fluorogenic cation sensors is scientifically exigent work. Recently chalcone derivatives gained more attention because of their structural variability. A suitable donor and acceptor groups separated by delocalized π-orbitals display excellent chromogenic and fluorogenic properties because of intramolecular charge transfer (ICT). These designed molecular frameworks provide the coordination sites to the incoming metal ions results in small changes in the optical properties. In a typical sensing behavior, coordination leads to a large conjugation plane with the probe resulted in hypo/hyperchromic shifts or red/blue shifts. In this review, we tried to converge the reported chalcone-derived sensors and explored the design, synthesis, metal ion sensing mechanism, and practical application of the probes. We expect that this review gives a basic outline for researchers to explore the field of chalcone-based sensors further.

Keywords: Chalcone; Chemosensor; Fluorescence; Metal ions; Pyrazoline.

Polycystic ovarian syndrome (PCOS) is caused by abnormal production of androgens resulting in the formation of small fluid-filled sacs in the ovaries. This condition worsens the life quality of women by disturbing their physiology and psychology in reproductive age. PCOS may also be associated with other morbidities like diabetes and hypertension. Numerous factors like imbalanced dietary patterns, poor lifestyle activities, improper care and medication, late diagnosis, and ignorance are involved in the prevalence of this disease in women. Hence, an early diagnosis and improved dietary and lifestyle management may improve the life quality and timely recovery of the patient from this disease. Various herbal extracts show a positive correlation in reducing the indicators associated with PCOS. This review discusses the effect of dietary and lifestyle practices on PCOS as prominent features. It has been recommended that a balanced diet with 40% energy from carbohydrates, 30% from fats, and 30% from protein with optimum physical activity could reduce severe PCOS symptoms and improve metabolic balance. Furthermore, recommendations for modification of diet and lifestyle activities are made which may positively influence the recovery from PCOS. PRACTICAL APPLICATIONS: Polycystic ovarian syndrome (PCOS) severely affects the life quality of suffering women. The dietary patterns, lifestyle activities, and co-medical conditions collectively affect the severity and related consequences of PCOS. This article provides sufficient information on dietary and lifestyle modifications to effectively manage this disease. Furthermore, dietitians, nutritionists, gynecologists, and obstetrics physicians can benefit from the information provided in it to understand and develop the management strategies to alleviate the disease symptoms and severity.

Keywords: androgens; cysts; dietary therapy; lifestyle management; polycystic ovarian syndrome.

Objective: This study aimed to evaluate prognostic outcomes of PVL-derived oral squamous cell carcinomas (P-OSCC) based on recurrence, new primary tumour, metastasis and survival information.

Study design: Five databases and grey literature were searched electronically with the following main keywords (proliferative verrucous leukoplakia, squamous cell carcinoma and malignant transformation) to answer the following review question: 'Are survival outcomes for P-OSCC worse?' based on the PECOS principle. The Joanna Briggs Institute Critical Appraisal tool was used to identify possible biases and assess the quality of each of the primary studies.

Results: A total of 21 articles met the inclusion criteria, and the results of this systematic review suggest that P-OSCC can recur and generate new primary tumours; however, metastases are rare. Thus, most patients remain alive for an average period of 5 years.

Conclusion: Apparently, P-OSCC has better clinical prognostic characteristics than conventional OSCC. There is a lack of information on the main prognostic outcomes of P-OSCC; therefore, specific studies must be performed to achieve a better comparison between P-OSCC and conventional OSCC progression.

Keywords: malignant transformation; metastasis; new primary tumors; new primary tumours; prognosis; proliferative verrucous leukoplakia; recurrence; survival; systematic review.

Objectives: The objective of the current review was to synthesize the literature on intersectionality relative to disparities across the cancer care continuum. A model to support future intersectional cancer research was proposed.

Methods: Web-based discovery services and discipline-specific databases were queried for both peer-reviewed and gray literature. Study screening and data extraction were facilitated through the Covidence software platform.

Results: Among 497 screened studies, 28 met study inclusion criteria. Most articles were peer-reviewed empirical studies (n = 22) that focused on pre-diagnosis/screening (n = 19) and included marginalized racial/ethnic (n = 22) identities. Pre-cancer diagnosis, sexual orientation and race influenced women's screening and vaccine behaviors. Sexual minority women, particularly individuals of color, were less likely to engage in cancer prevention behaviors prior to diagnosis. Race and socioeconomic status (SES) were important factors in patient care/survivorship with worse outcomes among non-white women of low SES. Emergent themes in qualitative results emphasized the importance of patient intersectional identities, as well as feelings of marginalization, fears of discrimination, and general discomfort with providers as barriers to seeking cancer care.

Conclusions: Patients with intersectional identities often experience barriers to cancer care that adversely impact screening, diagnosis, treatment, as well as survivorship. The use of an "intersectional lens" as a future clinical and research framework will facilitate a more multidimensional and holistic approach to the care of cancer patients.

Keywords: cancer; culturally congruent care; healthcare disparities; patient care; psycho-oncology; social determinants of health.

The advancements in flexible electronics call for invention of fiber-based electronic systems by surface modification or encapsulation. Here we present novel shear-flow-induced graphene nanosheets coating microfibers by integrating the dip coating approach with the microfluidic spinning method. The core hydrogel microfiber was first spun continuously from the microfluidic device, and the shear flow from the dip coating approach allowed formation of the thin graphene oxide (GO) nanosheet coating shell. Because the fluid components and flow rates in the microfluidic spinning together with the lifting speed in the dip coating approach are highly controllable, the morphology of the resultant microfibers could be precisely tailored, including the core-shell structure, conductivity, and thermal responsibilities. These features equipped the resultant microfibers with the potential of thermal and motion sensors, and their value in gesture indicators has also been explored. Microfibers generated from such a simple and controllable method could be versatile in flexible electronics.

Keywords: flexible electronics; graphene oxide; hydrogel; microfiber; microfluidics.

Single ventricle heart defects (SVHDs) are a severe type of congenital heart disease with poorly understood pathogenic mechanisms. New research using patient-specific induced pluripotent stem cells (iPSCs) as a cellular model is beginning to uncover genetic and cellular etiologies of SVHDs. Hypoplastic left heart syndrome (HLHS) is a type of SVHD that is characterized by an underdeveloped left ventricle and other malformations in the left side of the heart. Hypoplastic right heart syndrome (HRHS), the second type of SVHD, is characterized by an underdeveloped right heart, including malformed tricuspid and pulmonary valves. Despite a noticeable lack of research on SVHD, emerging technologies offer a promising future to further probe the genetic and cellular mechanisms of these diseases. Pediatric cardiovascular research is at the dawn of a new era in terms of what can be discovered with patient-specific iPSCs in conjunction with other technologies (e.g., organoids, single-cell genomics, CRISPR/Cas9 genome editing). In this review, we present recent approaches and findings utilizing patient-specific iPSCs to identify cellular mechanisms responsible for improper cardiac organogenesis in HLHS and HRHS.

Keywords: CRISPR/Cas9; hypoplastic left heart syndrome (HLHS); hypoplastic right heart syndrome (HRHS); induced pluripotent stem cells (iPSCs); organoids; single-cell genomics.

Keywords: cardiac magnetic resonance; computed tomography; echocardiography; right ventricle; tricuspid valve.

Background: Recent genome-wide studies revealed the molecular subtypes and mutational landscape of bladder cancer, which is the 10th most common cancer causing many deaths. ELF3 is one of the frequently mutated genes in bladder cancer with 14% alteration rate. It mainly functions as an epithelial transcription factor and its proper function is critical for the urothelium development. However, the impact of ELF3 mutations in bladder cancer is currently unknown.

Methods and results: In this study, we analysed the gene expression data available for primary bladder cancer and bladder cancer cell lines according to the mutation status of ELF3. Our results show that de-regulated genes common in cell lines and primary tissue are primarily involved in ameboidal type cell migration and cell-cell junction organization. Additionally, we identify that ELF3-mutant cases in primary samples significantly overexpress PIK3C2B and ELF3 and PIK3C2B and ELF3 are significantly co-mutated in many cancer types. Our integrative analysis with existing Hi-C data further revealed the genes proximally located to ELF3, including PIK3C2B to be upregulated in ELF3 mutant cases, potentially as a result of truncated ELF3 protein product and subsequent changes in regulatory interactions.

Conclusions: Our results provide important insights about how ELF3 mutation contributes to bladder tumorigenesis and uncover previously unknown dependencies.

Keywords: Bladder cancer; ELF3; Epithelial cell; PIK3C2B.

Visual working memory (VWM) is prone to interference from stored items competing for its limited capacity. Distinctiveness or similarity of the items is acknowledged to affect this competition, such that poor item distinctiveness causes a failure to discriminate between items sharing common features. In three experiments, we studied how the distinctiveness of studied real-world objects (i.e., whether the objects belong to the same or different basic categories) affects the retrieval of objects themselves (simple recognition) and object-location conjunctions (information about which object was where in a display, cued recall). In Experiments 1 and 2, we found that distinctiveness did not affect memories for objects or for locations, but low-distinctive objects were more frequently reported at "swapped" locations that originally contained other objects, showing object-location memory swaps. In Experiments 3 we found that observers swapped the location of a tested object with another object from the same category more frequently than with any of the objects from another category. This suggests that more similar studied objects cause more retrieval competition in object-location judgments than in simple recognition. Additionally, we discuss a possible role of categorical labeling of locations that can support object-location retrieval when the studied objects are highly distinct.

Keywords: memory distinctiveness; object-location memory; recognition memory; swap errors; visual working memory.

Tumor purity is the percentage of cancer cells within a tissue section. Pathologists estimate tumor purity to select samples for genomic analysis by manually reading hematoxylin-eosin (H&E)-stained slides, which is tedious, time consuming, and prone to inter-observer variability. Besides, pathologists' estimates do not correlate well with genomic tumor purity values, which are inferred from genomic data and accepted as accurate for downstream analysis. We developed a deep multiple instance learning model predicting tumor purity from H&E-stained digital histopathology slides. Our model successfully predicted tumor purity in eight The Cancer Genome Atlas (TCGA) cohorts and a local Singapore cohort. The predictions were highly consistent with genomic tumor purity values. Thus, our model can be utilized to select samples for genomic analysis, which will help reduce pathologists' workload and decrease inter-observer variability. Furthermore, our model provided tumor purity maps showing the spatial variation within sections. They can help better understand the tumor microenvironment.

Keywords: computational pathology; deep learning; digital histopathology; digital pathology; genomic sequencing; multiple instance learning; spatial omics; tumor microenvironment; tumor purity; whole-slide images.

A new series of thiosemicarbazone derivatives (1-11) were prepared from various aldehydes and isocyanates with high yields and practical methods. The structures of these compounds were elucidated by Fourier transform infrared, ¹ H-nuclear magnetic resonance (NMR), ¹³ C-NMR spectroscopic methods and elemental analysis. Cytotoxic effects of target compounds were determined by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide assay and compound 1 showed significant cytotoxic activity against both MCF-7 and MDA-MB-231 cells, with half-maximal inhibitory concentration values of 2.97 μM and 6.57 μM, respectively. Moreover, in this study, the anticholinergic and antidiabetic potentials of these compounds were investigated. To this aim, the effect of the newly synthesized thiosemicarbazone derivatives on the activities of acetylcholinesterase (AChE) and αglycosidase (α-Gly) was evaluated spectrophotometrically. The title compounds demonstrated high inhibitory activities compared to standard inhibitors with K_i values in the range of 122.15-333.61 nM for α-Gly (K_i value for standard inhibitor = 75.48 nM), 1.93-12.36 nM for AChE (K_i value for standard inhibitor = 17.45 nM). Antiproliferative activity and enzyme inhibition at the molecular level were performed molecular docking studies for thiosemicarbazone derivatives. 1M17, 5FI2, and 4EY6, 4J5T target proteins with protein data bank identification with (1-11) compounds were docked for anticancer and enzyme inhibition, respectively.

Keywords: Schiff base; antiproliferative activity; enzyme inhibition; molecular docking; thiosemicarbazone.

Metformin is the first-line drug to treat type 2 diabetes mellitus. Its mechanism of action is still debatable, and recent studies report that metformin attenuates oxidative stress. This study evaluated the in vitro antioxidant effects of a broad range of metformin concentrations on insulin-producing cells. The cell cycle, metabolism, glucose-stimulated insulin secretion, and cell death were evaluated to determine the biguanide effects on beta-cell function and survival. Antioxidant potential was based on reactive oxygen species (ROS), reduced glutathione (GSH), oxidative stress biomarker levels, and antioxidant enzyme and transcriptional factor Nrf2 activities. The results demonstrate that metformin disrupted GSIS in a concentration-dependent manner, lowered insulin content, and attenuated beta-cell metabolism. At high concentrations, metformin induced cell death and cell cycle arrest as well as increased ROS generation, consequently reducing GSH content. Although carbonylated protein content was elevated, indicating oxidative stress, the antioxidant enzyme and Nrf2 activities were not altered. In conclusion, our results show that metformin disrupts pancreatic beta-cell functionality but does not exert a putative antioxidant effect. It is important to note that the drug could potentially affect beta-cells, especially at high circulating levels.

Keywords: ROS; beta-cells; insulin secretion; metformin; oxidative stress.

Purpose: To evaluate the blood flow at the site of delta-shaped anastomosis during robotic distal gastrectomy and determine surgical outcomes and risk factors for ischemia at the anastomotic site from the perspective of clinical characteristics, surgical outcomes and perigastric vascular anatomy.

Methods: We included 55 patients who underwent robotic distal gastrectomy with a blood flow evaluation in the duodenal walls using intravenous indocyanine green injection with the da Vinci Xi-equipped Firefly system. Additional resection was performed in patients with a poor blood flow. Clinical characteristics, surgical outcomes and perigastric vascular anatomy were compared between the 45 patients with sufficient blood flow (group S) and the 10 patients with insufficient blood flow (group I). Vascular anatomy was assessed by preoperative contrast-enhanced computed tomography according to Hiatt's classification.

Results: The patient characteristics and surgical outcomes were not significantly different. No patient developed anastomotic complications requiring intervention; more patients in group I had aberrant branching of the left hepatic artery than those in group S (p = 0.047).

Conclusions: An indocyanine green-based blood flow evaluation might be an effective method of preventing anastomotic complications of delta-shaped anastomosis. Anatomical branching variations of the left hepatic artery might be a risk factor for impaired vascular perfusion of the anastomotic site.

Keywords: Delta-shaped anastomosis; Indocyanine green; Ischemia; Robotic distal gastrectomy; da Vinci FireFly system.

Tannins are an interesting class of polyphenols, characterized, in almost all cases, by a different degree of polymerization, which, inevitably, markedly influences their bioavailability, as well as biochemical and pharmacological activities. They have been used for the process of tanning to transform hides into leather, from which their name derives. For several time, they have not been accurately evaluated, but now researchers have started to unravel their potential, highlighting anti-inflammatory, antimicrobial, antioxidant and anticancer activities, as well as their involvement in cardiovascular, neuroprotective and in general metabolic diseases prevention. The mechanisms underlying their activity are often complex, but the main targets of their action (such as key enzymes modulation, activation of metabolic pathways and changes in the metabolic fluxes) are highlighted in this review, without losing sight of their toxicity. This aspect still needs further and better-designed study to be thoroughly understood and allow a more conscious use of tannins for human health.

Keywords: Complex tannins; Ellagitannins; Gallotannins; Pharmacological activities; Proanthocyanidins; Toxicology.

Hermit crabs have an intimate relationship with gastropod shells and show numerous activities by which they locate, select, and change shells in different contexts. They gather information about new shells and update information about their existing shells. This involves integration of different sensory modalities, memory-formation, and comparison of the overall value of each shell. Crabs also fight to get shells from other crabs, and again they gather information about the shell qualities and the opponent. Attacking crabs monitor their fight performance, and defenders are influenced by attacker activities, and both crabs are influenced by the gain or loss that might be made by swapping shells. Swapping shells involves the defender being naked for a short period. Leaving a shell also occurs if the shell is experimentally fixed in place or buried in sand or if small electric shocks are applied to the abdomen, and the quality of the current shell is traded-off against escaping possible asphyxiation or the aversive shocks. Hermit crabs show remarkable abilities, involving future planning, with respect to recognizing the shape and size of shells, and how they limit their passage through environmental obstructions. They also assess if shells might become available and wait for that to happen. Groups of crabs arrange themselves in size order so that orderly transfer of shells might occur down a line of crabs. These observations are discussed in the light of complex perceptual and cognitive abilities, and the possibility of sentience and awareness is discussed.

Keywords: Assessment; Cognition; Decision-making; Memory; Perception; Sentience.

Dr. Jose Delgado performed audacious demonstrations utilizing brain stimulation to instantly change behavior in animals. These feats spark ethical debates to this day. However, behind his controversial career is an important legacy of neurological discoveries and technological innovation. Delgado pioneered techniques in causally manipulating brain patterns and behavior with electrical stimulation and developed innovative, closed-loop neural devices. His inventive devices and techniques were ahead of his time and remain relevant to the field of neuromodulation today.

"My favorite saying is "there must be a use for my talent" … I chose chemistry as a career because chemical reactions are wonderful. Chemistry is everywhere in life and affects life all the time …" Find out more about Yawei Liu in her Introducing … Profile.

Patients with classic congenital adrenal hyperplasia due to 21-hydroxylase deficiency (21OHD) need life-long medical treatment to replace the lacking glucocorticoids and potentially lacking mineralocorticoids and to lower elevated adrenal androgens. Long-term complications are common, including gonadal dysfunction, infertility, and cardiovascular and metabolic co-morbidity with reduced quality of life. These complications can be attributed to the exposure of supraphysiological dosages of glucocorticoids and the longstanding exposure to elevated adrenal androgens. Development of novel therapies is necessary to address the chronic glucocorticoid overexposure, lack of circadian rhythm in glucocorticoid replacement, and inefficient glucocorticoid delivery with concomitant periods of hyperandrogenism. In this review we aim to give an overview about the current treatment regimens and its limitations and describe novel therapies especially evaluated for 21OHD patients.

Keywords: 21-hydroxylase deficiency; Adrenocorticotropic hormone antagonist; Congenital Adrenal Hyperplasia; Corticotropin-releasing hormone receptor antagonist; Glucocorticoid replacement therapy; Modified-release; Steroid production inhibitors.

Purpose of review: This review will discuss micropapillary urothelial carcinoma with respect to biology, histopathologic characteristics, genetic and molecular features, diagnosis, clinical management, and future directions of research.

Recent findings: Recent consensus opinion study showed only moderate interobserver reproducibility in the diagnostic criteria. The most reproducible criteria with the highest consensus were multiple nests in the same lacunar spaces. There are recent reports of high rates of intratumoral heterogeneity of ERBB2 amplification within tumor containing both micropapillary and classic urothelial components. Micropapillary urothelial carcinoma is a well-documented highly aggressive variant of urothelial carcinoma with proven worse outcomes. Accurate recognition and reporting of this pattern is critical for optimal management. Newer therapeutic strategies related to the molecular and genetic findings seen in MPUC remain to be explored further.

Keywords: Aggressive variant; Bladder; Lacunae; Management; Micropapillary; Morphology; Prognosis; Urothelial carcinoma.

In recent years, cannabinoid (CB) products have gained popularity among the public. The anti-inflammatory properties of CBs have piqued the interest of researchers and clinicians because they represent promising avenues for the treatment of autoimmune and inflammatory skin disorders that may be refractory to conventional therapy. The objective of this study was to review the existing literature regarding CBs for dermatologic conditions. A primary literature search was conducted in October 2020, using the PubMed and Embase databases, for all articles published from 1965 to October 2020. Review articles, studies using animal models, and nondermatologic and pharmacologic studies were excluded. From 248 nonduplicated studies, 26 articles were included. There were 13 articles on systemic CBs and 14 reports on topical CBs. Selective CB receptor type 2 agonists were found to be effective in treating diffuse cutaneous systemic sclerosis and dermatomyositis. Dronabinol showed efficacy for trichotillomania. Sublingual cannabidiol and Δ-9-tetrahydrocannabinol were successful in treating the pain associated with epidermolysis bullosa. Available evidence suggests that CBs may be effective for the treatment of various inflammatory skin disorders. Although promising, additional research is necessary to evaluate efficacy and to determine dosing, safety, and long-term treatment guidelines.

Keywords: 2-AG, 2-arachidonoylglycerol; ACR-CRISS, American College of Rheumatology-combined response index in diffuse cutaneous systemic sclerosis; AEA, anandamide; CB, cannabinoid; CB1R, cannabinoid receptor 1; CB2R, cannabinoid receptor 2; CBD, cannabidiol; CDASI, cutaneous dermatomyositis disease area and severity index; DM, dermatomyositis; ECS, endocannabinoid system; KC, keratinocyte; MRSS, modified Rodnan skin thickness score; N-PEA, N-palmitoylethanolamide; QOLHEQ, Quality of Life Hand Eczema Questionnaire; RCT, randomized controlled trial; SSc, systemic sclerosis; VAS, Visual Analog Score; Δ9-THC, delta-9-tetrahydrocannabinol.

Reactive oxygen species (ROS; free radical form O₂ ^•‾ , superoxide radical; OH^• , hydroxyl radical; ROO^• , peroxyl; RO^• , alkoxyl and non-radical form ¹ O₂ , singlet oxygen; H₂ O₂ , hydrogen peroxide) are inevitable companions of aerobic life with crucial role in gut health. But, overwhelming production of ROS can cause serious damage to biomolecules. In this review, we have discussed several sources of ROS production that can be beneficial or dangerous to the human gut. Microorganisms, organelles and enzymes play crucial role in ROS generation, where, NOX1 is the main intestinal enzyme, which produce ROS in the intestine epithelial cells. Previous studies have reported that probiotics play significant role in gut homeostasis by checking the ROS generation, maintaining the antioxidant level, immune system and barrier protection. With current knowledge, we have critically analyzed the available literature and presented the outcome in the form of bubble maps to suggest the probiotics that help in controlling the ROS-specific intestinal diseases, such as inflammatory bowel disease (IBD) and colon cancer. Finally, it has been concluded that rebooting of the gut microbiota with probiotics, postbiotics or fecal microbiota transplantation (FMT) can have crucial implications in the structuring of gut communities for the personalized management of the gastrointestinal (GI) diseases.

Keywords: Reactive oxygen species; dysbiosis; fecal microbiota transplantation; gut microbiota; inflammatory bowel disease; probiotics.

Macroalgae and seagrasses in coastal habitats are exposed to turbulent water currents and waves that deform them and can rip them off the substratum, but that also transport essential water-borne substances to them and disperse their propagules and wastes. Field studies of the physical environment, ecological interactions, and life history strategies of marine macrophytes reveal which aspects of their biomechanical performance are important to their success in different types of natural habitats and enable us to design ecologically relevant laboratory experiments to study biomechanical function. Morphology and tissue mechanical properties determine the hydrodynamic forces on macrophytes and their fate when exposed to those forces, but different mechanical designs can perform well in the same biophysical habitat. There is a trade-off between maximizing photosynthesis and minimizing breakage, and some macrophytes change their morphology in response to environmental cues. Water flow in marine habitats varies on a wide range of temporal and spatial scales, so diverse flow microhabitats can occur at the same site. Likewise, the size, shape, and tissue material properties of macrophytes change as they grow and age, so it is important to understand the different physical challenges met by macrophytes throughout their lives.

Keywords: Algae; biomechanics; ecomechanics; hydrodynamics; kelp; life history; marine macrophyte; plasticity; safety factor; seagrass.