We investigate a novel design for all-solid large mode area (LMA) leakage channel fibers (LCFs) for high-power Yb-doped fiber lasers and amplifiers, based on a single down-doped-silica rod ring surrounding a seven-cell pure-silica core, aiming for effectively single-mode behavior and low bending loss characteristics. Through detailed numerical simulations based on the finite element method (FEM), we find that the proposed all-solid LMA-LCFs, having a seven-cell core and two different sizes of down-doped rods, can achieve sufficient differential mode loss and much lower bending loss, as compared with a previously-reported LCF with a one-cell core and six large down-doped-silica rods.

We demonstrate for the first time a flat-top mode from a 50 µm-core Yb-doped leakage channel fiber (LCF). The flat intensity distribution leads to an effective mode area of ~1880 µm(2) in the straight fiber, an over 50% increase comparing to that of regular LCF with the same core diameter. The flat-top mode was achieved by using a uniform Yb-doped silica glass in the core center with an index of ~2 × 10(-4) lower than that of the silica background. The fiber was also tested in a laser configuration, demonstrating an optical-to-optical efficiency of ~77% at 1026 nm with respect to the pump at 975 nm.

During an investigation of yeast biota in the rhizosphere of lentisk in Sardinian semi-arid areas, a strain was isolated that could not be assigned to any known species. The sequence of the D1/D2 domain of the large subunit rDNA gene revealed that the strain belonged to the genus Kazachstania and was phylogenetically related to a clade including Kazachstania aerobia, Kazachstania servazzii, Kazachstania solicola and Kazachstania unispora. The novel isolate differed from members of this clade in its ability to assimilate D-glucono-1,5-lactone and its very weak fermentation of glucose and sucrose; its assimilation profile was unique within the genus Kazachstania. Monosporal colonies were able to sporulate, indicating that the species is homothallic. It is proposed that the isolate represents a novel species, Kazachstania ichnusensis sp. nov., with LCF 1675(T) (=CBS 11859(T)) as type strain.

Stocky galaxias Galaxias tantangara is a newly-described freshwater fish restricted to a single population, occupying a 3 km reach of a small headwater stream in the upper Murrumbidgee River catchment of south eastern Australia. Listed as critically endangered under IUCN Red List criteria, knowledge of the species' ecology is critical for future conservation efforts to establish additional populations by translocation and captive breeding. This study details the first account of spawning and reproductive ecology of G. tantangara, including reproductive development, timing of spawning and a description of one spawning site. Peak gonadosomatic index was observed in March/April in males and October in females. Absolute fecundity ranged from 211 oocytes for a 76 mm LCF fish, to 810 oocytes for a 100 mm LCF fish. The observation of spent females in mid-November 2017, and discovery of an egg mass eight days later suggests spawning had occurred, and over a relatively short period. Larvae were subsequently detected in monthly electrofishing surveys in December 2017. Findings from this study provide new understanding of existing and future threats to G. tantangara and has important implications for conservation management of not only this species, but also other closely related threatened Galaxias species. This article is protected by copyright. All rights reserved.

Keywords: Galaxias tantangara; conservation; freshwater; incubation; life history; reproduction.

Intercellular interactions amongst eosinophils and adjacent cells are involved in the recruitment and functioning of eosinophils within tissues. For their mobilization from the vasculature and adherence to endothelial cells, eosinophils utilize mechanisms dependent on CD18 as well as on binding to either endothelial leukocyte adhesion molecule-1 or vascular cell adhesion molecule-1 (VCAM). Eosinophils, unlike neutrophils, express VLA-4, the ligand mediating adhesion to VCAM. Eosinophil recruitment is also promoted by lymphokines, including lymphocyte chemoattractant factor (LCF) and interleukin-2 (IL-2), both of which are about 1,000 times more potent than either PAF or C5a as eosinophil chemoattractants. As on lymphocytes and monocytes, LCF utilizes CD4 on eosinophils as its receptor; and IL-2 stimulated migration is mediated by apparent high affinity IL-2 receptors on eosinophils. Eosinophils recruited into tissues may have multiple functional capabilities, including the capacity to elaborate cytokines active on other cells, including TGF-α, TGF-β1 and IL-1α, and the capacity to serve as MHC-restricted, HLA-DR-dependent antigen-presenting cells to stimulate proliferative responses of CD4+ lymphocytes.

Keywords: Antigen presentation; CD4; ELAM; Eosinophils; Interleukin-1α; Interleukin-2; Lymphocyte chemoattractant factor; Transforming growth factor-α; Transforming growth factor-β; VCAM; VLA-4.

The bioaccessibility of As in soil, rather than its total concentration, is closely related to its potential risk. In this study, the in situ formation of amorphous Fe oxides was applied to As-contaminated soil to induce As-Fe coprecipitates that can withstand the gastric digestion condition of human beings. To promote the formation of Fe oxides, 2% ferric nitrate (w/w) and 30% water (v/w) were introduced, and the pH was adjusted to ~7. The chemical extractability of As in soil was determined using the solubility/bioavailability research consortium method and five-step sequential extraction. In situ formation of Fe oxides resulted in a remarkable increase in the As associated with amorphous Fe oxides, decreasing most of the exchangeable As (i.e., the sum of SO₄^2- and PO₄^3- extractable As), and thereby reducing the bioaccessibility of As. The types of association between As and Fe oxides were investigated using X-ray absorption spectroscopy analysis. Linear combination fit (LCF) analysis demonstrated that As bound to amorphous Fe oxides could exist as coprecipitates with ferrihydrite and schwertmannite after stabilization. The bioaccessibility of the coprecipitated As in soil further decreased as amorphous Fe oxides transformed to crystalline form with time, which was supported by the LCF results showing an increase of goethite in aged soil.

Keywords: As-Fe coprecipitates; Fe oxide crystallization; In situ Fe oxide formation; Oral bioaccessibility; XAS (X-ray absorption spectroscopy).

Arterial hypertension (AHT) is a leading cardiovascular disease, with a high negative impact on the quality of life. Crinum zeylanicum (C. zeylanicum) leaves extract is used in the West region of Cameroon to treat AHT and heart problems. This study aimed to investigate the antihypertensive effect of C. zeylanicum extract in N ^ω -nitro-L-arginine methyl ester- (L-NAME-) induced hypertensive rats. The aqueous extract of C. zeylanicum (LAE) was obtained by lyophilizing the juice of triturated fresh leaves. The methanol extract (LME) prepared by maceration of the dried leaves was further partitioned to chloroform (LCF), ethyl acetate (LEAF), and residual (LRF) fractions. The total polyphenol, flavonoid content, and antiradical potentials of these extracts were determined. The curative antihypertensive and renal function protective effects of LME and LEAF were evaluated in vivo on L-NAME-induced hypertensive rats. Hypertension was induced in rats by oral administration of L-NAME (30 mg/kg/day) for 3 consecutive weeks. Thereafter, plant extracts were administered orally at the doses of 30, 60, and 120 mg/kg/day, concomitantly with L-NAME for three other weeks. Body weight, heart rate, and arterial blood pressure were measured at the end of each week throughout the experimental period. At the end of the treatment, 24-hour urine and plasma were collected to assay nitric oxide (NO), creatinine, and protein. The results revealed that LEAF has the higher content of total polyphenol and flavonoid and exhibited the best antiradical potential. Moreover, treatment of hypertensive rats with LME and LEAF significantly (p < 0.001) reduced AHT and heart rate. LME and LEAF significantly increased rat's body mass, plasmatic NO, and urinary creatinine and reduced urine NO and protein contents as compared to the L-NAME group. LME and its LEAF possess potent antihypertensive effects and further protect the renal function in L-NAME-induced hypertensive rats, thus supporting the use of C. zeylanicum in the management of AHT.

The fatigue behaviors of metals are different under different in-service environment and loading conditions. This study was devoted to investigating the combined effects of high and low cycle fatigue loads on the performance of the low alloy steel Q345. Three kinds of experiments were carried out, including the pure high cycle fatigue (HCF) tests, the pure low cycle fatigue (LCF) tests, and the combined high and low cycle fatigue (HLCF) tests. The prediction formulae were proposed for the combined high and low cycle fatigue failure. Scanning electron microscopy (SEM) and stereo microscope were used to analyze the microstructure and fracture morphology due to different fatigue loads. Case study on the combined high and low cycle fatigue damage of a steel arch bridge was carried out based on the FE method and the proposed formula. The results show that the LCF life decreases evidently due to the prior HCF damages. The HLCF fracture surface is relatively flat near the crack initiation side, and rugged at the other half part. The fatigue damages at the bridge joints increase significantly with consideration of the pre-fatigue damages caused by traffic load. In the 100th anniversary of service, the fatigue damage index without considering the HCF pre-damage is only about 50% of the coupled damage value.

Keywords: combined high and low cycle fatigue; high cycle fatigue; low alloy steel; low cycle fatigue; material experiment; structural performance.

A HILIC-ESI^--MS coupled with MAMA depolymerization is proposed here for unusual discrimination and characterization of plant polysaccharides: a case study of fruit polysaccharides in SCP and SSP. The optimized MAMA hydrolysis procedure was proposed for sample preparations of low-polymerization saccharides (M_w < 5000 Da) released in SCP and SSP. In addition, HILIC-MS/MS was employed for elucidation of isomeric glycosidic linkages in terms of ¹⁸O labelling. The MAMA hydrolysates showed that the amount of neutral →(⁴Hex¹)_n→ moiety is confirmed to be more bigger than that of acidic →(⁴HexA¹)_n → in SCP, whereas the amount of acidic →(⁴HexA¹)_n→ moiety seems to be more bigger than that of neutral →(⁴Hex¹)_n→ in SSP. The resulting LCF showed the performance on rapid visualization of SCP and SSP by HILIC-MIM-MS. PCA and HCA further unveils several key Q-markers (e.g., m/z 503, 369, 665, 827, 989, 1151 and 735) for rapid discrimination of SCP and SSP. This practical study showed that the LCF with PCA and HCA could effectively reflect structural differences and could rapidly achieve discrimination of SCP and SSP.

Keywords: HILIC-ESI(−)-MS; Low-polymerization compositional fingerprinting; Microwave assisted mild acid hydrolysis; Schisandra polysaccharides.

Selective laser melting (SLM) is a promising additive manufacturing (AM) process for high-strength or high-manufacturing-cost metals such as Ti-6Al-4V widely applied in aeronautical industry components with high material waste or complex geometry. However, one of the main challenges of AM parts is the variability in fatigue properties. In this study, standard cyclic fatigue and monotonic tensile testing specimens were fabricated by SLM and subsequently heat treated using the standard heat treatment (HT) or hot isostatic pressing (HIP) methods. All the specimens were post-treated to relieve the residual stress and subsequently machined to the same surface finishing. These specimens were tested in the low-cycle fatigue (LCF) regime. The effects of post-process methods on the failure mechanisms were observed using scanning electron microscopy (SEM) and optical microscopy (OM) characterization methods. While the tensile test results showed that specimens with different post-process treatment methods have similar tensile strength, the LCF test revealed that no significant difference exists between HT and HIP specimens. Based on the results, critical factors influencing the LCF properties are discussed. Furthermore, a microstructure-based multistage fatigue model was employed to predict the LCF life. The results show good agreement with the experiment.

Keywords: SLM; additive manufacturing; fatigue model; low-cycle fatigue; titanium alloy.

Lipid based formulations (LBFs) are commonly employed to enhance the absorption of highly lipophilic, poorly water-soluble drugs. However, the utility of LBFs can be limited by low drug solubility in the formulation. Isolation of ionizable drugs as low melting, lipophilic salts or ionic liquids (ILs) provides one means to enhance drug solubility in LBFs. However, whether different ILs benefit from formulation in different LBFs is largely unknown. In the current studies, lumefantrine was isolated as a number of different lipophilic salt/ionic liquid forms and performance was assessed after formulation in a range of LBFs. The solubility of lumefantrine in LBF was enhanced 2- to 80-fold by isolation as the lumefantrine docusate IL when compared to lumefantrine free base. The increase in drug loading subsequently enhanced concentrations in the aqueous phase of model intestinal fluids during in vitro dispersion and digestion testing of the LBF. To assess in vivo performance, the systemic exposure of lumefantrine docusate after administration in Type II-MCF, IIIB-MCF, IIIB-LCF, and IV formulations was evaluated after oral administration to rats. In vivo exposure was compared to control lipid and aqueous suspension formulations of lumefantrine free base. Lumefantrine docusate in the Type IIIB-LCF showed significantly higher plasma exposure compared to all other formulations (up to 35-fold higher). The data suggest that isolation of a lipid-soluble IL, coupled with an appropriate formulation, is a viable means to increase drug dose in an oral formulation and to enhance exposure of lumefantrine in vivo.

Exploring optimal strategies to improve patient outcome postoperatively is still under challenge. Cancer immunotherapy has great potential to prevent the postoperative tumor recurrence and metastasis, which could be further strengthened by re-education of tumor microenvironment (TME). Herein, a local and sustained drug delivery system of liquid crystal formation system (LCFS) co-loaded with doxorubicin (DOX) and resiquimod (R848) (D/R@LCFS) is reported to confer effective chemoimmunotherapy with reduced systematic toxicity. After local administration, D/R@LCFS turns tumor into in situ vaccine via DOX-triggered immunogenic cell death effect accompanied with immunostimulatory effect of R848. Meanwhile, combination treatment of D/R@LCFS facilitates the recruitment of effector CD8⁺ T cells and the polarization of myeloid-derived suppressor cells and immunosuppressive type 2-polarized macrophages to tumoricidal antigen-presenting cells, favoring antigen-specific T cell immune response and inducing more immunogenic phenotypes in tumors. The generated in situ vaccine as well as reshaped TME by D/R@LCFS elicited systematic immune response and long term immune-memory effect in combination with immune checkpoint blockade to significantly prevent postoperative B16F10 or 4T1 tumor recurrence and metastasis. Therefore, this combination strategy of spatiotemporal TME modulation is expected to provide a clinical available option for effective postoperative chemoimmunotherapy.

Keywords: cancer chemoimmunotherapy; in situ vaccine; liquid crystal formation system; postoperative treatment; tumor microenvironment.

Using anesthetized mongrel dogs exposed to 60 min of ligation of the left anterior descending coronary artery followed by 60 min of reperfusion, we examined the effect of superoxide dismutase (SOD) and dimethylthiourea (DMTU) on evidence of endothelial injury in coronary rings studied in vitro. In 13 dogs treated with saline rings from the normal left circumflex coronary artery (LCF) relaxed by 98 +/- 4% when exposed to 10(-5) M acetylcholine whereas rings from the left anterior descending coronary artery (LAD) relaxed by 79 +/- 7% (p less than 0.05). In the same rings maximum relaxation with the ionophore A23187 was 107 +/- 5% versus 87 +/- 8% (p less than 0.05) for the LCF and the LAD, respectively. Comparisons of concentration-response curves through a range of doses of both acetylcholine and A23187 revealed significant differences for both vasodilators between the LCF and the LAD (p less than 0.01 for each). Nine dogs were treated with bovine SOD infused in the left atrium the last 20 min of ligation and throughout reperfusion (140 units/kg/min) and six other dogs were treated with DMTU 500 mg/kg i.v. given the last 30 min of the ligation period. Neither SOD nor DMTU prevented endothelial injury in the LAD. Despite pretreatment with these agents, there were significant reductions in maximum relaxation and in total concentration-response curves in the LAD as compared with the results in rings from the LCF with both acetylcholine and A23187. There were normal responses to nitroprusside in both the LCF and LAD in all three experimental groups.(ABSTRACT TRUNCATED AT 250 WORDS)

To compare the element compositions between intact (i.e., intact throughout its length) and disrupted (i.e., ligament no longer attached to the attachment) ligaments, the contents of elements in the human ligamenta capitum femorum (LCF) were analyzed by inductively coupled plasma-atomic emission spectrometry. Histological and immunohistological assessments were also performed in both groups. The subjects were 8 men and 32 women. Trace element analyses showed that the sulfur and iron contents were significantly greater in the intact group than in the disrupted group, while the phosphorus and magnesium contents were significantly smaller in the intact group than in the disrupted group. The calcium and zinc contents were smaller in the intact group than in the disrupted group, with no significant differences. Histologically, there were fibrocartilage cells and extracellular matrix metachromasia in ligaments of the intact group. In contrast, fibrocartilage cells disappeared, and fat cells appeared instead of collagen fibrils in ligaments of the disrupted group. The LCFs of the intact group were immunohistologically positive for all components examined including collagens, glycosaminoglycans, and proteoglycans. The increase in sulfur suggested the presence of high glycosaminoglycan levels associated with fibrocartilaginous metaplasia in the ligament by compressive force. The reduction in iron may show a decreased number of blood vessels in the synovium after ligament disruption. The increases in phosphorus, magnesium, and calcium are indicative of degenerative changes including calcification and ossification. We conclude that differences in the contents of elements between intact and disrupted LCFs indicate degenerative alterations to the ligament structure after disruption.

Chylous ascites is the leakage of lipid-rich lymph into the peritoneal cavity usually due to disruption of lymphatics or increased peritoneal lymphatic pressure. Various surgical options have been proposed to treat chylous ascites but most have shown suboptimal outcomes. The gastroepiploic vascularized lymph node (GE-VLN) flap has been described previously for the treatment of lymphedema. In chylous ascites, this flap could provide an alternate drainage pathway for the intraperitoneal chylous fluid. The purpose of this report is to present another option for the microsurgical treatment of refractory chylous ascites. Herein, we report two patients with refractory chylous ascites secondary to cancer who have undergone deep inferior epigastric-based lymphatic "cable" flap (DIE-LCF) connected to a pedicle GE-VLN flap. Patients were followed-up for a minimum of 2 years. Within the first 3 months following surgery, the patient's nutritional parameters improved along with drastic reduction of ascites. At 2 years follow-up postoperative abdominal circumference decreased significantly. None required further peritoneal paracentesis and all patients were free of chylous ascites symptoms. In conclusion, the DIE-LCF connected to a pedicle GE-VLN flap could be a feasible option for the microsurgical treatment of refractory chylous ascites.

The disabling effects of traumatic brain injuries (TBI) present a significant healthcare concern to developed countries. In order to achieve a reliable prognosis, validated assessment scales are used to monitor the cognitive outcome, like the Level of Cognitive Functioning Scale, or the overall functional outcome, namely the Functional Independence Measure and Glasgow Outcome Scale. The aim of our study was to evaluate the role of Level of Cognitive Functioning Scale (LCF) as an outcome prognostic index in patients with TBI.

Materials and methods: Fifty-four patients with TBI with a mean age of 44.9 years (SD 20.915) were enrolled in this retrospective study. Patients were evaluated at admission and at discharge using the Glasgow Outcome Scale, Functional Independence Measure, and Level of Cognitive Functioning Scale. The Glasgow Outcome Scale was also implemented at 6 months after discharge (OUTCOME.GOS), whereas the LCF was used twice a week throughout hospitalization. For our purpose, we named LCF at admission LCFa, whereas permanence in the same LCF value (number of days), LCFaL.

Results: Δ.GOS, Δ.FIM (Δ = difference between value at discharge and at admission), and OUTCOME.GOS were significantly affected by age, length of stay, LCFa, and LCFaL.

Conclusion: The LCF can give a valuable indication to the prognosis of patients with TBI besides monitoring changes in cognitive function. This allows for individual rehabilitation plan, and long-term management strategies could be developed more quickly upon patient's discharge. Consequently, valuable healthcare and social care resources could be assigned correctly.

Keywords: Level of cognitive functioning; Outcome scale; Prognosis; Rehabilitation; Traumatic brain injury.

Two surgical approaches, an anterolateral and a posterolateral approach, have been advocated for lateral condylar fractures (LCFs) of the humerus in children. The purpose of this study was to evaluate the radiographic and clinical outcomes of the 2 surgical approaches.

Methods: We retrospectively analyzed the data of consecutive patients <15 years of age with an LCF treated via open reduction and internal fixation through 1 of 2 surgical approaches during the period of April 2000 to March 2019. Patients were classified into the anterolateral (AL) and posterolateral (PL) groups, according to the surgical approach used. Postoperative complications and radiographic and clinical findings (including range of motion and findings on the basis of the Flynn criteria) were investigated. To investigate humeral deformity, the Baumann angle and the carrying angle were measured on anteroposterior radiographs.

Results: Sixty-one of 82 patients met the inclusion criteria. The AL group included 17 patients (13 male, 4 female), and the PL group included 44 patients (28 male, 16 female). In the PL group, 7 patients had cubitus varus deformity, 3 had malunion due to unacceptable reduction of fracture fragments, and 6 had elbow joint contracture. In the AL group, the overall clinical results were excellent for 15 patients and good for 2. In the PL group, the clinical results were excellent for 12 patients, good for 14, fair for 6, and poor for 12.

Conclusions: An anterolateral approach would be the optimal approach for an LCF in pediatric patients.

Level of evidence: Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.

Background: Severe infectious complications are a frequent problem in patients with disability due to a severe acquired brain injury. Previous studies reported that the rehabilitation outcome is significantly lower in patients colonized or infected. However, these results could be influenced by comorbidities of those patients admitted in rehabilitation hospital with a lower functional status.

Aim: To explore the influence of systemic infection, in particular concerning multi-drug resistant bacteria and analyze the role of comorbidities, as a risk factor for the development of systemic infection, on rehabilitation outcomes in patients with severe brain injury.

Design: This research is a cohort, prospective-observational study, comparing patients with and without systemic infections, in terms of rehabilitation outcomes .

Settings: An Italian Intensive Care Rehabilitation department.

Population: A group of 221 patients (mean age: 59 years, range: 16-93 years, 127 males, 94 females) with severe acquired brain injury admitted to rehabilitation hospital.

Methods: We compared the rehabilitation outcomes between patients with and without a systemic infection (at least a positive blood culture) during the rehabilitation period. A secondary analysis was performed on 70 patients with infection versus 70 patients without infection, matched for functional status at admission. The used clinical scores were: Cumulative Illness Rating Scale for Geriatrics (CIRS-G), Coma Recovery Scale Revised (CRS-R), Glasgow Coma Scale (GCS), Functional Independence Measure (FIM), Glasgow Outcome Scale (GOS), Disabilty Rating Scale (DRS), Levels of Cognitive Functioning (LCF) administered at admission and discharge. Length of hospitalization and the role of comorbidities were also considered.

Results: The group of patients with systemic infection (in particular due to Gram-negative bacteria) had a significantly lower outcome for 5 out 6 clinical scales and with a more than doubled length of hospitalization (p<0.001). However, these patients with, at least, a positive blood culture resulted having lower functional status at admission. In the secondary analysis, worst outcome was found in patients with positive blood culture in terms of FIM (p=0.033), GOS (p=0.048), and CRS-R (p=0.001).

Conclusions: Systemic infections during rehabilitation increased the length of hospitalization and reduce the rehabilitative outcomes, even when the analysis was performed on groups matched for the functional status at admission. Moreover, the cardiological and endocrine- metabolic comorbidities seem to influence the outcome, without represent a further risk factor for systemic infection.

Clinical rehabilitation impact: The impact of infections during rehabilitation inpatient should be more taken into account, with specific procedures and suitable environments for avoiding the diffusions of infections.

This study evaluated by micro-computed tomography (μCT) the filling ability in curved root canals, besides the flow of AH Plus (AHP) and Neo MTA Plus (NMTAP) sealers using different methodologies. Mandibular molars mesial roots with two root canals and degree of curvature between 20° and 40° were selected. The specimens were prepared with the ProDesign R system up to size 35.05 and were filled with the sealers by a continuous wave of condensation technique, Thermo Pack II (n=12). The teeth were scanned using μCT after root canal preparation and obturation. The volumetric percentage of filling material and voids were calculated. Flow was evaluated based on ISO 6876/2012 (n=10). Flow and filling were also evaluated in μCT using a glass plate with a central cavity and four grooves from the central cavity (n=6). Flow was linearly calculated into the grooves. The central cavity filling (CCF) and lateral cavity filling (LCF) were calculated in mm³. Data were submitted to non-paired t test with a significance threshold at 5%. The percentage of filling and voids between the root canals filled with AHP or NMTAP was similar (p>0.05). NMTAP presented the lowest flow in conventional test (p<0.05). Using μCT, sealers had similar CCF, LCF and linear flow (p>0.05). In conclusion, NMTAP and AHP had similar filling ability in curved mesial root canals of mandibular molars without presence of isthmus. Although AHP presented better flow than NMTAP using ISO methodology, there was no difference between these materials regarding volumetric filling when evaluated by μCT.

Malaria -typhoid co-infection is associated with poverty and underdevelopment with significant morbidity and mortality with similarities in clinical features of the two diseases that often result in misdiagnosis and mistreatment of the febrile patients. The Co-administration of artemether lumefantrine (AL) with ciprofloxacin as treatment for malaria-typhoid co-infection is common in Nigeria and this increases risk of pharmacokinetic drug-drug interaction since ciprofloxacin is an inhibitor of CYP3A4 that metabolizes AL. In an open-label prospective three arm design with registration pactr201909811770922, one hundred and nineteen (119) febrile volunteers comprising 55 males and 64 females were distributed into three oral treatment regimen groups. Group 1 consist of sixty-five participants presenting malaria mono infection treated with AL only and fifty-four participants presenting malaria-typhoid co-infection randomly assigned to Group 2 treated with AL and ciprofloxacin concomitantly and Group 3 whose doses were staggered at 2 hours interval. Blood samples were collected from participants in the three groups on 3 different days: day 0 (before commencement of treatment); day 3 (after completion of AL); and day 7 (after completion of ciprofloxacin), The collected blood sample were used to determine parasite density, serum liver and kidney function parameters, haematological indices, and day 7 lumefantrine concentration. The data in this article provides the changes in PCR-uncorrected Early Treatment Failure (ETA), Late Clinical Failure (LCF), Late Parasitological Failure (LPF), Day 7 serum lumefantrine concentration, liver and kidney function parameters, axillary body temperature and PCV/Hb associated with the different treatment regimen. The dataset [1] as a baseline, will stimulate the need for a randomized clinical assessment of the efficacy of AL when co-administered with ciprofloxacin in the treatment regimen of Malaria-typhoid co-infection in endemic areas. Such findings are capable of influencing national treatment policy on the management of malaria-typhoid co-infection.

Keywords: Artemether lumefantrine; Ciprofloxacin; Day 7 Lumefantrine; Efficacy; Malaria-Typhoid.

The purpose of the study was to evaluate the clinical features and outcomes of lens capsule fragment (LCF) adherent to the posterior corneal surface after cataract surgery. Methods A total of 12 eyes from 12 patients were included with a mean follow-up duration of 19.4 ± 12.6 months. Demographics and clinical features were collected by reviewing medical records and slitlamp photographs. Outcome parameters included corrected distance visual acuity (CDVA), central corneal thickness, and anterior segment optical coherence tomography (AS-OCT) features. Results All LCF located centrally and remained fixed and turned to semitransparent in a mean time of 28.7 ± 20.1 days. The AS-OCT revealed an extra membrane at the posterior corneal surface, with an underlying intact Descemet membrane in 9 eyes. All patients experienced corneal edema associated with LCF, which was medically managed and resolved in a mean time of 58.1 ± 40.2 days. CDVA improved from logarithm of minimum angle of resolution scores of 0.91 ± 0.63 preoperatively to 0.25 ± 0.18 at 2 months after surgery. None of the patients expressed subjective visual complaints. Conclusions LCF adherent to the posterior corneal surface caused prolonged corneal edema after cataract surgery, but exhibited no clinically significant complications in the midterm follow-up. AS-OCT provided useful diagnostics and differentiating features.

Keywords: Anterior segment optical coherence tomography; Corneal edema; Descemet membrane detachment; Lens capsule; Phacoemulsification.

Following a successful study on the prediction of fatigue life of high-heat-load components made of Glidcop, the thermal limitation of oxygen-free copper (OFC), which is used more commonly than Glidcop, has been studied. In addition to its general mechanical properties, the low-cycle-fatigue (LCF) and creep properties of OFC were investigated in detail and compared with those of Glidcop. The breaking mode of OFC, which was observed to be completely different from that of Glidcop in a fatigue fracture experiment, clarified the importance of considering the creep-fatigue interaction. An additional LCF test with compressive strain holding was conducted so that the creep-fatigue life diagram for out-of-phase thermal fatigue could be obtained on the basis of the strain-range partitioning method. The life predicted from elasto-plastic creep analysis agreed well with that determined from the void ratio estimated in the fatigue fracture experiment.

Beam deflection experiments were used to systematically examine size effects on the low cyclic fatigue (LCF) deformation behaviour of micro-sized bending beams of copper (Cu) single crystals oriented for single slip, critical and coplanar double slip. We present cyclic hardening curves and fatigue surface roughness, as well as dislocations structures of the micro-sized beams with sizes between 1 and 15 µm. A clear crystal orientation and size effect on the cyclic hardening curves, surface roughness, and the dislocation microstructures were observed. Based on the experimental results, the fatigue damage in single slip orientations clearly decreased with decreasing the sample size, however, below a critical size regime, the surface damage suddenly increases. Additionally, samples with sizes larger than 5 µm clearly revealed, besides PSBs-like structures, the emergence of kink bands leading to larger surface roughness in comparison to the smaller ones. Fatigue surface damages in microcrystals oriented for critical double slip became more prevalent compared to single slip orientations. Quantitatively, the correlation of the fatigue surface damage was also demonstrated with the formation of PSBs-like structures.

Orthopaedic implants experience large cyclic loads, and pre-clinical analysis is conducted to ensure they can withstand millions of loading cycles. Acetabular cup developments aim to reduce wall thickness to conserve bone, and this produces high pre-stress in modular implants. As part of an implant development process, we propose a technique for preclinical fatigue strength assessment of modular implants which accounts for this mean stress, stress concentrating features and material processing. A modular cup's stress distributions were predicted computationally, under assembly and in vivo loads, and its cyclic residual stress and stress amplitude were calculated. For verification against damage initiation in low-cycle-fatigue (LCF), the peak stress was compared to the material's yield strength. For verification against failure in high-cycle-fatigue (HCF) each element's reserve factor was calculated using the conservative Soderberg infinite life criterion. Results demonstrated the importance of accounting for mean stress. The cup was predicted to experience high cyclic mean stress with low magnitude stress amplitude: a low cyclic load ratio (Rl=0.1) produced a high cyclic stress ratio (Rs=0.80). Furthermore the locations of highest cyclic mean stress and stress amplitude did not coincide. The minimum predicted reserve factor Nf was 1.96 (HCF) and 2.08 (LCF). If mean stress were neglected or if the stress ratio were assumed to equal the load ratio, the reserve factor would be considerably lower, potentially leading to over-engineering, reducing bone conservation. Fatigue strength evaluation is only one step in a broader development process, which should involve a series of verifications with the full range of normal and traumatic physiological loading scenarios, with representative boundary conditions and a representative environment. This study presents and justifies a fatigue analysis methodology which could be applied in early stage development to a variety of modular and pre-stressed prosthesis concepts, and is particularly relevant as implant development aims to maximise modularity and bone conservation.