Underwater sounds are very important in social communication of harp seals (Pagophilus groenlandicus) because they are the main means of long- and short-distance communication. Individual harp seals must try to avoid being masked and emit only those calls that will benefit them. Underwater vocalizations of harp seals were recorded during the breeding season. The physical characteristics associated with antimasking attributes of 16 call types were examined. Rising frequency or increasing amplitude within calls were not common. Most of the calls ended abruptly (range 145-966 dB/s), but call onset was more gradual. At high calling rates (95.1-135 calls/min) there were significantly more calls overlapping temporally than at medium (75.1-95 calls/min) or low (35-75 calls/min) calling rates, but even at the highest calling rates, 79.1% of the calls were not overlapped. When 2, 3, or 4 calls overlapped, there were significantly fewer frequency separations of less than 1/3 octave than would be expected by chance. This is important because sounds that are separated by less than 1/3 octave likely mask each other. When 2-4 calls are occurring simultaneously, only 4.5% to 14.2% are masked by virtue of being within 1/3 octave from their nearest neighbor. None of the overlappping calls was of the same type. This suggests that the seals are actively listening to each other's calls and are not randomly using the different call types. Harp seals use frequency and temporal separation in conjunction with a wide vocal repertoire to avoid masking each other.

The ability of a listener to recognize sound sources, and in particular musical instruments from the sounds they produce, raises the question of determining the acoustical information used to achieve such a task. It is now well known that the shapes of the temporal and spectral envelopes are crucial to the recognition of a musical instrument. More recently, Modulation Power Spectra (MPS) have been shown to be a representation that potentially explains the perception of musical instrument sounds. Nevertheless, the question of which specific regions of this representation characterize a musical instrument is still open. An identification task was applied to two subsets of musical instruments: tuba, trombone, cello, saxophone, and clarinet on the one hand, and marimba, vibraphone, guitar, harp, and viola pizzicato on the other. The sounds were processed with filtered spectrotemporal modulations with 2D Gaussian windows. The most relevant regions of this representation for instrument identification were determined for each instrument and reveal the regions essential for their identification. The method used here is based on a "molecular approach," the so-called bubbles method. Globally, the instruments were correctly identified and the lower values of spectrotemporal modulations are the most important regions of the MPS for recognizing instruments. Interestingly, instruments that were confused with each other led to non-overlapping regions and were confused when they were filtered in the most salient region of the other instrument. These results suggest that musical instrument timbres are characterized by specific spectrotemporal modulations, information which could contribute to music information retrieval tasks such as automatic source recognition.

Ronald et al. (1977) suggested that blood flow in the caudal/lumbar sections of the extradural intravertebral vein (EIV) of seals changes direction from running towards the head before diving, to the opposite during diving. The possible advantage would be that the oxygen-depleted venous effluent from the brain is routed via the EIV to the posterior parts of the hepatic sinuses and the inferior caval vein and, hence, is prevented from mixing with the more oxygen-rich venous blood in their anterior parts. We have re-examined this hypothesis by use of Doppler flowmetry. A catheter-tip flow probe was introduced into the EIV of two similar-sized juvenile harp seals, and flow direction and rate determined before, during and after simulated dives lasting for 5 min, at three positions (caudal, lumbar and thoracic) along the EIV. Regardless of probe position, blood was mainly flowing towards the head in 11 of 13 experiments prior to diving, in 8 of 13 experiments during diving and in 11 of 13 experiments during recovery after diving (and away from the head in the remaining experiments). Flow direction was most variable in the caudal position. Mean blood velocity in the EIV was substantially lower during diving (0.10 +/- 0.22 cm s-1 (n=5) in thoracic position) than in the pre-dive (3.98 +/- 3.32 cm s-1 [n=5]) and post-dive (5.75 +/- 4.07 cm s-1 [n=5]) situations. Thus, the direction and rate of flow in the EIV was variable, particularly during diving, as is to be expected in a system of anastomosing, valveless veins. We conclude that the hypothesis of Ronald et al. (1977) most likely is false.

BACKGROUND

Myocardial motion is an important observable for the assessment of heart condition. Accurate estimates of ventricular (LV) wall motion are required for quantifying myocardial deformation and assessing local tissue function and viability. Harmonic Phase (HARP) analysis was developed for measuring regional LV motion using tagged magnetic resonance imaging (tMRI) data. With current computer-aided postprocessing tools including HARP analysis, large motions experienced by myocardial tissue are, however, often intractable to measure. This paper addresses this issue and provides a solution to make such measurements possible.

METHODS

To improve the estimation performance of large cardiac motions while analyzing tMRI data sets, we propose a two-step solution. The first step involves constructing a model to describe average systolic motion of the LV wall within a subject group. The second step involves time-reversal of the model applied as a spatial coordinate transformation to digitally relax the contracted LV wall in the experimental data of a single subject to the beginning of systole. Cardiac tMRI scans were performed on four healthy rats and used for developing the forward LV model. Algorithms were implemented for preprocessing the tMRI data, optimizing the model parameters and performing the HARP analysis. Slices from the midventricular level were then analyzed for all systolic phases.

RESULTS

The time-reversal operation derived from the LV model accounted for the bulk portion of the myocardial motion, which was the average motion experienced within the overall subject population. In analyzing the individual tMRI data sets, removing this average with the time-reversal operation left small magnitude residual motion unique to the case. This remaining residual portion of the motion was estimated robustly using the HARP analysis.

CONCLUSIONS

Utilizing a combination of the forward LV model and its time reversal improves the performance of motion estimation in evaluating the cardiac function.

A comparison of identified faunal assemblages from the Dorset site of Phillip's Garden indicates that harp seal hunting was the main focus of activity throughout the site's occupation. Despite the highly specialized nature of site use, it appears that reliance on harp seal decreased over time while fish and birds became increasingly important. These changes may reflect longer seasonal occupations at the site in later centuries, and/or a decrease in the local availability of harp seal. The observed shift coincides with the onset of a local climatic warming trend, which might have affected harp seal movements in the area. Dorset subsistence and settlement patterns in Newfoundland are still poorly understood due to a lack of preserved faunal assemblages in the region. The temporal trend illustrated here indicates that we cannot assume that these patterns were static throughout the Dorset occupation of the island.

Concentrations of 22 elements were determined in blubber, liver, kidney and muscle of five male and five female, 8-month-old harp seals (Phoca groenlandica) by ICP-MS. Young harp seals are hunted during the spring and fall and represent an important traditional dietary item for some northern fishing communities. Interest in the commercial use of seal meat products and the limited data on the level of contaminants in tissues of harp seal beaters motivated our investigation. For most elements, concentrations in liver or kidney were greater than those in muscle. Blubber concentrations were generally lowest, but concentrations of Li, As, Sr, Ba and Pb were highest in blubber. Largest concentration factors [(tissue)/(water)>> 10,000] were seen for P, Fe, Zn, Cu, Cd, Se, Mn, Ag, Pb and Co, a list that includes essential elements such as Fe and Zn, as well as several important contaminants such as Cd and Pb. Differences in concentration between male and female seals were only seen in eight of 88 element/tissue comparisons. Any effects of environmental exposure due to location or dietary intake are difficult to detect. Principle component analysis shows an association of Ca with Sr and P with S; and also a distinct grouping of the elements V, Mo, Cu and Ag.

As part of a study on the effects of pollutants on forests, a long-term monitoring programme has been operating at two sites (602 and 275 m above sea level (a.s.l.)) in Glentress Forest, south-east Scotland, since June 1987. At these sites, equipment has been recording 20-min data for weather parameters, and cloud and rain event frequency, duration and intensity. The chemical composition of bulk cloud and rainwater has been measured. Cloudwater detection and collection has been made using passive 'Harp wire' gauges (with a cross-sectional area of 0.047 m2) strung with polypropylene filament and having a typical collection efficiency of 29% when compared with independent measurements of windspeed and liquid water content. During 1988, the annual rainfall at the upper site was 1213 mm, occurring over 1776 h. The equivalent cloudwater deposition to a forest with a drag coefficient of 0.06 was estimated to be 375 mm over 1936 h. A 'typical cloud event' lasted 4.5 h and would deposit to forests at a rate of 0.2 mm h(-1). There were significantly higher loadings of suspended particulate material >> 0.2 microm) in cloudwater (mean 18.42, max. 94.5 mg litre(-1)) compared with rainwater (mean 2.6, max. 25.6 mg litre(-1)). There were similar differences in ion concentrations, e.g. for H+ in cloudwater (mean 163, max. 1259 microm) and in rainwater (mean 33, max. 262 microM). It is concluded that cloudwater deposition represents a major pathway for pollutant transfer to the upland environment, especially where the surface vegetation is efficient at capturing cloudwater, i.e. forests. The consequences of this increased pollutant loading to forests and water catchments are yet to be assessed.

OBJECTIVE

The aim was to evaluate the use of combination of SBT (sequence based typing) and HARP (heterozygous ambiguity resolving primer) in HLA typing to acquire high resolution typing results.

METHODS

167 DNA samples were analyzed by SBT. The web site HARPs Finder provided by Conexio Genomics, the developer of HARPs (http://www.harpsfinder.conexio-genomics.com/index.html) was then used to search for appropriate HARPs.

RESULTS

HARPs can resolve 95% of ambiguities for locus A; 86% for B and 60% for DRB1 locus. However, there are still limitations. Practically PCR products of un-separated alleles are used as templates for sequencing by HARP; sometimes, it is still impossible to get unambiguous typing.

CONCLUSIONS

We outlined the advantages and disadvantages of SBT/HARP. A list of HARPs for choice to resolve ambiguity of SBT in Taiwanese population is concluded.

Male grigs, bush crickets and crickets produce mating calls by tegminal stridulation: the scraping together of modified forewings functioning as sound generators. Bush crickets (Tettigoniidae) and crickets (Gryllinae) diverged some 240 million years ago, with each lineage developing unique characteristics in wing morphology and the associated mechanics of stridulation. The grigs (Prophalangopsidae), a relict lineage more closely related to bush crickets than to crickets, are believed to retain plesiomorphic features of wing morphology. The wing cells widely involved in sound production, such as the harp and mirror, are comparatively small, poorly delimited and/or partially filled with cross-veins. Such morphology is similarly observed in the earliest stridulating ensiferans, for which stridulatory mechanics remains poorly understood. The grigs, therefore, are of major importance to investigate the early evolutionary stages of tegminal stridulation, a critical innovation in the evolution of the Orthoptera. The aim of this study is to appreciate the degree of specialization on grig forewings, through identification of sound radiating areas and their properties. For well-grounded comparisons, homologies in wing venation (and associated areas) of grigs and bush crickets are re-evaluated. Then, using direct evidence, this study confirms the mirror cell, in association with two other areas (termed 'neck' and 'pre-mirror'), as the acoustic resonator in the grig Cyphoderris monstrosa Despite the use of largely symmetrical resonators, as found in field crickets, analogous features of stridulatory mechanics are observed between C. monstrosa and bush crickets. Both morphology and function in grigs represents transitional stages between unspecialized forewings and derived conditions observed in modern species.

Lipoproteins and the impact of lipid lowering on progression and regression of coronary artery disease are discussed. Angiographically assessed regression studies are reviewed (NHLBI, LIT, LHT, CLAS I and II, FATS, POSCH, Heidelberg, STARS, SCRIP, MAAS, PLAC I, HARP, UC-SF), as are B-mode ultrasound studies (ACAPS, PLAC II) and survival studies (Oslo diet-smoking study, SSSS, Pravastatin, Oxford). Although study populations and the interventions are different in the studies, I have come to the following conclusions. Regression of atherosclerosis correlates well with reduction in LDL cholesterol and an increase in HDL cholesterol. Although overall improvement in the severity and extent of the disease was modest, reduction of clinical events was impressive. Lipid modulation may stabilize existing lesions by improving the stability of the lesion cap and/or promoting loss of cholesterol content from within the plaque. Survival studies indicate that lipid lowering lowers morbidity and increases longevity in patients with established coronary heart disease. The B-mode ultrasound studies using the carotid artery as surrogate for the change in atherosclerosis in the coronary seems extremely promising. The atherosclerotic process as well as complications may be studied at an early stage using noninvasive methods.

The number of harp seals, Phoca groenlandica (Eodeben 1777) may now range from 2.25 million to 3 million. The total population is divided among three separate breeding stocks in the White Sea, the Greenland Sea, and the Northwest Atlantic. The continued exploitation of the Northwest Atlantic stock has caused controversy, primarily because of public concern for the fate of the newborn "whitecoat." The harp seal's life-cycle is marked by a progression from on-ice birth to in-water mating and subsequent on-ice molt. An extensive migration follows, taking these animals northward to high Arctic summering grounds and southward in an autumnal return migration to the breeding grounds. Harp seals are efficient divers and possess well-developed microsensory perceptions associated with anatomical adaptation to their environment. The relation between our understanding of the basic biology of the species and the confrontation that occurs between these mammals and man is considered with respect to our technological invasion of the North, the regulated commercial kill, and the slow movement toward multispecies management. Sound decisions regarding the exploitation of this species can only be made with a knowledge of its biology.

OBJECTIVE

Layer specific strain assessment is increasingly being employed clinically. Cardiac magnetic resonance (MR) Feature Tracking (FT) is considered to be an adequate alternative for strain assessment. The aim of this study is to investigate the feasibility of FT derived layer specific strain assessment.

METHODS

CSPAMM and SSFP-Cine sequences were acquired in 38 individuals (19 patients with HFpEF, 19 controls) in identical midventricular short-axis locations. Global endocardial-, midmyocardial-, epicardial- peak systolic circumferential strain (PSCS) and regional epicardial PSCS were calculated and intra- as well inter-observer variability were assessed.

RESULTS

FT derived global epicardial and endocardial PSCS (7.9±2.3%; -19.6±4.9%) were significantly lower than tagging derived global epicardial and endocardial PSCS (-13.2±2.8%; -32.3±5.9%) (each p<0.001), while FT derived endocardial PSCS and tagging derived midmyocardial PSCS showed a strong correlation (r=0.71) and no significant differences. Global intra- and inter-observer variability of FT derived endocardial PSCS circumferential measures were acceptable (coefficient of variation 6.5% and 5.7%) while reproducibility of epicardial PSCS (coefficient of variation 16.8% and 18.1%) was poor.

CONCLUSIONS

The FT algorithm allows for reliable assessment of midmyocardial strain, while underestimating epicardial and endocardial strain and delivering less reproducible results than the gold standard of tagging.

The respiratory characteristics of mitochondria isolated from the subcutaneous brown adipose tissue of newborn harp seals indicate that the tissue is thermogenically active. Temperature recordings in vivo revealed, in fact, that the tissue was maintained at a temperature close to that of the body core during immersion of the pups in ice-water. Beta-adrenergic blockade markedly increased the cooling rates at both locations in ice-water, while curarization, accompanied by artificial respiration did not. We conclude that nonshivering thermogenesis through activated brown adipose tissue plays a decisive role in the defence against cold in the newborn harp seal.

A male yearling harp seal (Phoca groenlandica) stranded and was brought to Mystic Aquarium & Institute for Exploration's Seal Rescue and Rehabilitation Center. The seal presented with a bilateral pendular vertical nystagmus, negative menace response, and a positive palpebral response. Ophthalmological examination by slit lamp biomicroscopy revealed perilimbal corneal edema, excessive iridal surface structures, pupils that appeared to be shaped improperly (dyscoria), and suspected cataracts. Attempts to dilate the pupils with both dark-lighted conditions and repeated dosages of 10% phenylephrine and 1% atropine ophthalmic solution in each eye (OU) were unsuccessful. Ocular ultrasonography findings suggested bilateral cataracts with flattened anterior-posterior (A-P) diameter and possible persistent hyperplastic primary vitreous. It is possible that these structural congenital abnormalities could produce further ocular complications for this seal including uveitis, secondary glaucoma, retinal detachment, and/or vitreal hemorrhage in the future. This case demonstrates the importance of a thorough ophthalmological examination in stranded wild animals, especially if their symptoms appear neurological.