Phytochemical investigation on the concentrate of Huangjing wine, resulted in the isolation of three new tyrosol derivatives 4<i>'''</i>-hydroxyphenethyl 2-(R)-hydroxy-3-phenylpropionate (<b>1</b>), 4<i>'''</i>-hydroxyphenethyl(4<i>'</i>-hydroxy-3<i>'</i>-methoxyphenyl)propionate (<b>2</b>) and 4<i>''</i>-hydroxyphenethyl ethyl succinate (<b>3</b>), together with 5 known compounds, ferulic acid (<b>4</b>), L-phenyllactic acid (<b>5</b>), hydroxytyrosol (<b>6</b>), dihydroferulic acid (<b>7</b>), cyclo(L-Pro-D-Tyr) (<b>8</b>). Their structures were elucidated using spectroscopic analysis and by comparison with the literature data. All compounds displayed antioxidant effect in the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical. Among them, the new compound <b>2</b> exhibited obvious antioxidant effect, and new compounds <b>1</b> and <b>3</b> exhibited medium antioxidant effect.

Keywords: Huangjing wine; anti-oxidation; extraction and isolation; structural identification; tyrosol derivative.