The objective of this study was to profile the chemical components and biological activities analysis of crude extract of Bryophyllum pinnatum and Oxalis corniculata . Results revealed that the analyzed plant materials encompass the high amount of total phenolic and flavonoids content and have significant antioxidant activities. Furthermore, methanol extracts are the potential source of α- amylase, α- glucosidase, lipase, tyrosinase and elastase inhibitors. High resolution mass spectrometry revealed the presence of diverse metabolites such as Quercetin 3-O-alpha-L-rhamnopyranoside, Myricetin 3-rhamnoside, Bersaldegenin 1, 3, 5-orthoacetate, Bryophyllin C, Syringic acid, Caffeic acid, p-Coumaric acid, and Quercetin in B. pinnatum and Isoorientin, Swertisin, Apigenin 7,4'-diglucoside, Vitexin, 4-Hydroxybenzoic acid, Vanillic acid, Ethyl gallate, 5,7-Dimethoxy-3,3',4'-trihydroxyflavone, and Diosmetin-7-O-beta-D-glucopyranoside in O. corniculata. Our finding suggested that these two plant species have high medicinal importance and are potential source of inhibitors for modern pharmaceuticals, nutraceuticals and cosmetics industries.