Abstract:

A reliable method has been developed for the determination of total serum T3, dialyzable fraction (DFT3), and absolute concentration of free T3 (AFT3). Total T3 values (mean +/- SD) were: healthy euthyroid subjects, 0.33 +/- 0.07 mug per 100 ml; hyperthyroid patients, 0.71 +/- 0.1 mug per 100 ml; hypothyroid, 0.10 +/- 0.03 mug per 100 ml. Values (mean +/- SD) for DFT3 in these groups were 0.46 +/- 0.14%, 0.78 +/- 0.17%, and 0.16 +/- 0.08%, respectively. Calculated values for AFT3 were: 1.51 +/- 0.4 mmug per 100 ml, 5.00 +/- 0.6 mmug per 100 ml and 0.24 +/- 0.1 mmug per 100 ml, respectively. Dilution of serum before dialysis lowered estimated DFT3 values. Enrichment of serum with labeled T3 in the range examined did not affect DFT3. However, DFT3 was increased by addition of Merthiolate to serum in concentration 1: 10,000 due to displacement of T3 from thyroxine-binding globulin to albumin. The data suggest that triiodothyronine may play a considerably more important role in normal and pathological physiology, as evidenced by kinetic analysis using these data. A metabolic role for T3 equal to that of T4 is indicated.

Open in

PUBMED | DOI | PMC | Google Scholar | Wikipedia

Relations:

Content

Citations

(24)

References

(17)

Drugs

(1)

Affiliates

(1)

Total and Free Triiodothyronine in Human Serum<sup><a href="#fn1" rid="fn1" class=" fn">*</a></sup>

J Nauman

A Nauman

S Werner

Abstract

A reliable method has been developed for the determination of total serum T3, dialyzable fraction (DFT3), and absolute concentration of free T3 (AFT3). Total T3 values (mean ± SD) were: healthy euthyroid subjects, 0.33 ± 0.07 μg per 100 ml; hyperthyroid patients, 0.71 ± 0.1 μg per 100 ml; hypothyroid, 0.10 ± 0.03 μg per 100 ml. Values (mean ± SD) for DFT3 in these groups were 0.46 ± 0.14%, 0.78 ± 0.17%, and 0.16 ± 0.08%, respectively. Calculated values for AFT3 were: 1.51 ± 0.4 mμg per 100 ml, 5.00 ± 0.6 mμg per 100 ml and 0.24 ± 0.1 mμg per 100 ml, respectively. Dilution of serum before dialysis lowered estimated DFT3 values. Enrichment of serum with labeled T3 in the range examined did not affect DFT3. However, DFT3 was increased by addition of Merthiolate to serum in concentration 1: 10,000 due to displacement of T3 from thyroxine-binding globulin to albumin. The data suggest that triiodothyronine may play a considerably more important role in normal and pathological physiology, as evidenced by kinetic analysis using these data. A metabolic role for T3 equal to that of T4 is indicated.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.7M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Image
on p.1349

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

GROSS J, PITT-RIVERS R. The identification of 3:5:3'-L-triiodothyronine in human plasma. Lancet. 1952 Mar 1;1(6705):439–441. [PubMed] [Google Scholar]
ROCHE J, LISSITZKY S, MICHEL R. Sur la présence de triiodothyronine dans la thyroglobuline. C R Hebd Seances Acad Sci. 1952 Mar 10;234(11):1228–1230. [PubMed] [Google Scholar]
BENUA RS, DOBYNS BM, NINMER A. Triiodothyronine in the serum of patients treated with radioactive iodine. J Clin Endocrinol Metab. 1955 Nov;15(11):1367–1378. [PubMed] [Google Scholar]
ARONS WL, HYDOVITZ JD. The serum pattern of thyroid hormones in euthyroidism and hyperthyroidism. J Clin Endocrinol Metab. 1959 May;19(5):548–556. [PubMed] [Google Scholar]
SHIMAOKA K, JASANI BM. THE APPLICATION OF TWO-DIMENSIONAL PAPER CHROMATOGRAPHY AND LOW-LEVEL COUNTING TO THE STUDY OF TRIIODOTHYRONINE IN PLASMA. J Endocrinol. 1965 Apr;32:59–64. [PubMed] [Google Scholar]
PIND K. Paperchromatographic determination of thyroid hormone (3,5,3'-triiodothyronine) in serum without radio-iodine. Acta Endocrinol (Copenh) 1957 Nov;26(3):263–272. [PubMed] [Google Scholar]
Barker SB. Further studies on the ceric sulphate-arsenious acid reaction for the detection of various analogues of thyroxine. Biochem J. 1964 Jan;90(1):214–219.[PMC free article] [PubMed] [Google Scholar]
ROBBINS J, RALL JE. Proteins associated with the thyroid hormones. Physiol Rev. 1960 Jul;40:415–489. [PubMed] [Google Scholar]
INGBAR SH, FREINKEL N. Regulation of the peripheral metabolism of the thyroid hormones. Recent Prog Horm Res. 1960;16:353–403. [PubMed] [Google Scholar]
Ingbar SH, Braverman LE, Dawber NA, Lee GY. A new method for measuring the free thyroid hormone in human serum and an analysis of the factors that influence its concentration. J Clin Invest. 1965 Oct;44(10):1679–1689.[PMC free article] [PubMed] [Google Scholar]
Scriba PC, Heinze HG, Landgraf R, Frey KW, Schwarz K. Klinische Bedeutung der Bestimmung der Bindung von Trijodthyronin an Serumproteine mittels Dextran-Gel-Filtration. Klin Wochenschr. 1966 Feb 1;44(3):131–147. [PubMed] [Google Scholar]
MANDL RH, BLOCK RJ. Methods for the qualitative, semiquantitative and quantitative determination of iodoamino acids and of inorganic iodide in iodoprotein digests and in human serum. Arch Biochem Biophys. 1959 Mar;81(1):25–35. [PubMed] [Google Scholar]
MURPHY BE, PATTEE CJ. DETERMINATION OF THYROXINE UTILIZING THE PROPERTY OF PROTEIN-BINDING. J Clin Endocrinol Metab. 1964 Feb;24:187–196. [PubMed] [Google Scholar]
Sterling K, Brenner MA. Free thyroxine in human serum: simplified measurement with the aid of magnesium precipitation. J Clin Invest. 1966 Jan;45(1):153–163.[PMC free article] [PubMed] [Google Scholar]
OPPENHEIMER JH, SURKS MI. DETERMINATION OF FREE THYROXINE IN HUMAN SERUM: A THEORETICAL AND EXPERIMENTAL ANALYSIS. J Clin Endocrinol Metab. 1964 Aug;24:785–793. [PubMed] [Google Scholar]
West CD, Chavré VJ, Wolfe M. A serum thyroxine method: application in thyroid disease and iodine-treated patients. J Clin Endocrinol Metab. 1965 Sep;25(9):1189–1195. [PubMed] [Google Scholar]
FISHER DA, ODDIE TH. WHOLE-BODY COUNTING OF 131-I-LABELED TRIIODOTHYRONINE. J Clin Endocrinol Metab. 1964 Aug;24:733–739. [PubMed] [Google Scholar]

Department of Medicine, Columbia University College of Physicians & Surgeons and The Presbyterian Hospital in the City of New York

^{International postdoctoral research fellow (2-F05-TW-843-01), U. S. Public Health Service. Permanent address: II Department of Medicine, Postgraduate Medical School, Warsaw, Poland.}

^{Permanent address: II Department of Medicine, Postgraduate Medical School, Warsaw, Poland.}

^{Address all reprint requests to Dr. Sidney C. Werner, Department of Medicine, 630 West 168th Street, New York, N. Y. 10032.}

^{Submitted for publication March 3, 1967; accepted May 18, 1967.}

This study was aided by grant AM 00008 (C17 and 18), National Institutes of Health, U. S. Public Health Service.

Copyright notice

Abstract

A reliable method has been developed for the determination of total serum T3, dialyzable fraction (DFT3), and absolute concentration of free T3 (AFT3). Total T3 values (mean ± SD) were: healthy euthyroid subjects, 0.33 ± 0.07 μg per 100 ml; hyperthyroid patients, 0.71 ± 0.1 μg per 100 ml; hypothyroid, 0.10 ± 0.03 μg per 100 ml. Values (mean ± SD) for DFT3 in these groups were 0.46 ± 0.14%, 0.78 ± 0.17%, and 0.16 ± 0.08%, respectively. Calculated values for AFT3 were: 1.51 ± 0.4 mμg per 100 ml, 5.00 ± 0.6 mμg per 100 ml and 0.24 ± 0.1 mμg per 100 ml, respectively. Dilution of serum before dialysis lowered estimated DFT3 values. Enrichment of serum with labeled T3 in the range examined did not affect DFT3. However, DFT3 was increased by addition of Merthiolate to serum in concentration 1: 10,000 due to displacement of T3 from thyroxine-binding globulin to albumin. The data suggest that triiodothyronine may play a considerably more important role in normal and pathological physiology, as evidenced by kinetic analysis using these data. A metabolic role for T3 equal to that of T4 is indicated.

Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.

Learn More