Tissue transglutaminase as the autoantigen of coeliac disease.
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Journal: 1997/December - Gut
ISSN: 0017-5749
PUBMED: 9391261
Abstract:
Dieterich et al show that immunoprecipitation of human fibrosarcoma cell lysates (cell line HT1080) using the IgA fraction from serum samples of patients with coeliac disease results in a single protein band with a molecular weight of 85 kDa. Immunoprecipitation occurred exclusively with 25 coeliac disease serum samples, but with none of the 25 control samples. The 85 kDa antigen was cleaved with endoproteinase Asp-N, and after amino-terminal sequence analysis, the three cleavage products tested all yielded sequences that could be assigned to tissue transglutaminase (EC 2.3.2.13; tTG). In order to prove that tTG obtained from the fibrosarcoma cells binds to the endomysial antibody fraction of coeliac serum IgA, the authors performed indirect immunofluorescence with high titre coeliac disease serum samples on monkey oesophagus with or without prior incubation of those samples with a commercially available tTG extract (Sigma, Deisenhofer, Germany). Pretreatment with tTG almost completely abolished endomysial antibody labelling. Also, when the Sigma tTG was used as antigen in an enzyme linked immunosorbent assay (ELISA), 12 coeliac disease patient samples but none of the seven control serum samples displayed increased IgA immunoreactivity. Dieterich et al conclude they have identified tTG as the unknown endomysial autoantigen.
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Gut 41(4): 565-566
PMID: 9391261

Tissue transglutaminase as the autoantigen of coeliac disease

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  • Popović OS, Kostić KM, Milović VB, Milutinović-Djurić S, Miletić VD, Sesić L, Djordjević M, Bulajić M, Bojić P, Rubinić M, et al. Primary bile acid malabsorption. Histologic and immunologic study in three patients. Gastroenterology. 1987 Jun;92(6):1851–1858. [PubMed] [Google Scholar]
  • Milutinović-Djurić S, Popović OS, Milović V, Necić D, Kostić KM. Postcholecystectomy diarrhea from villous atrophy of the terminal ileum. J Clin Gastroenterol. 1993 Apr;16(3):227–230. [PubMed] [Google Scholar]
  • Veress B, Löfberg R, Bergman L. Microscopic colitis syndrome. Gut. 1995 Jun;36(6):880–886.[PMC free article] [PubMed] [Google Scholar]
  • Bitoun A, Thérond JP, Nisard A, Bernier JJ. Apport de l'endoscopie au diagnostic de la maladie coeliaque de l'adulte. Gastroenterol Clin Biol. 1981 Mar;5(3):282–286. [PubMed] [Google Scholar]
  • Isaacs P, Whittaker SM, Turnberg LA. Diarrhea associated with medullary carcinoma of the thyroid. Studies of intestinal function in a patient. Gastroenterology. 1974 Sep;67(3):521–526. [PubMed] [Google Scholar]
  • Flourié B, Leblond A, Florent C, Rautureau M, Bisalli A, Rambaud JC. Starch malabsorption and breath gas excretion in healthy humans consuming low- and high-starch diets. Gastroenterology. 1988 Aug;95(2):356–363. [PubMed] [Google Scholar]
  • Williams AJ, Merrick MV, Eastwood MA. Idiopathic bile acid malabsorption--a review of clinical presentation, diagnosis, and response to treatment. Gut. 1991 Sep;32(9):1004–1006.[PMC free article] [PubMed] [Google Scholar]
  • Zeroogian JM, Chopra S. Collagenous colitis and lymphocytic colitis. Annu Rev Med. 1994;45:105–118. [PubMed] [Google Scholar]
  • Pimentel RR, Achkar E, Bedford R. Collagenous colitis. A treatable disease with an elusive diagnosis. Dig Dis Sci. 1995 Jun;40(6):1400–1404. [PubMed] [Google Scholar]
  • Hamilton I, Sanders S, Hopwood D, Bouchier IA. Collagenous colitis associated with small intestinal villous atrophy. Gut. 1986 Nov;27(11):1394–1398.[PMC free article] [PubMed] [Google Scholar]
  • Austin LL, Dobbins WO. Studies of the rectal mucosa in coeliac sprue: the intraepithelial lymphocyte. Gut. 1988 Feb;29(2):200–205.[PMC free article] [PubMed] [Google Scholar]
  • Cadiot G, Flourie B, Galian A, Lavergne A, Modigliani R. Maladie coeliaque et colite collagène. Une association fortuite? Presse Med. 1990 Oct 27;19(35):1621–1622. [PubMed] [Google Scholar]
  • Wolber R, Owen D, Freeman H. Colonic lymphocytosis in patients with celiac sprue. Hum Pathol. 1990 Nov;21(11):1092–1096. [PubMed] [Google Scholar]
  • Lewis FW, Warren GH, Goff JS. Collagenous colitis with involvement of terminal ileum. Dig Dis Sci. 1991 Aug;36(8):1161–1163. [PubMed] [Google Scholar]
  • Järnerot G, Tysk C, Bohr J, Eriksson S. Collagenous colitis and fecal stream diversion. Gastroenterology. 1995 Aug;109(2):449–455. [PubMed] [Google Scholar]
  • Rampton DS, Baithun SI. Is microscopic colitis due to bile-salt malabsorption? Dis Colon Rectum. 1987 Dec;30(12):950–952. [PubMed] [Google Scholar]
  • Andersen T, Andersen JR, Tvede M, Franzmann MB. Collagenous colitis: are bacterial cytotoxins responsible? Am J Gastroenterol. 1993 Mar;88(3):375–377. [PubMed] [Google Scholar]
  • Sciarretta G, Furno A, Morrone B, Malaguti P. Absence of histopathological changes of ileum and colon in functional chronic diarrhea associated with bile acid malabsorption, assessed by SeHCAT test: a prospective study. Am J Gastroenterol. 1994 Jul;89(7):1058–1061. [PubMed] [Google Scholar]
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