Eleven analytes were presented in the Urine Chemistry Survey Program during the year 1977. These included amylase, calcium, phosphorus, sodium, potassium, creatinine, osmolality, 17-ketosteroids, total protein, glucose, and urea nitrogen. More than 450 laboratories participated. Deficiencies in assay performance were found for amylase, 17-ketosteroids, and total protein. Sharp shifts in precision performance were seen in urea nitrogen analysis. Severe biases are apparent in glucose analysis using neocuproine and ferricyanide methods.