Abstract:

1. A computerized technique is described for the quantitative determination of radiometabolites from incubation studies. 2. Seven steroid substrates have been incubated with human endometrial tissue. The principal radiometabolites were identified and determined after 2hr. incubation without the addition of cofactors and after 4hr. incubation with cofactors. 3. The main products from progesterone were 20alpha-dihydroprogesterone and 5alpha-pregnanedione with lower yields of 5beta-pregnanedione and 20beta-dihydroprogesterone. There was no evidence for 17alpha-hydroxylase activity. 4. 17alpha-Hydroxyprogesterone was transformed into small yields of 17alpha,20alpha- and 17alpha,20beta-dihydroxypregn-4-en-3-one. In one incubation there was evidence for conversion into androstenedione. 5. Dehydroepiandrosterone was transformed into small amounts of androstenedione, 5alpha-androstanedione and androsterone. 6. Androstenedione and testosterone were interconvertible, the reaction favouring the formation of androstenedione. 5alpha-Androstanedione and androsterone were formed from both substrates. There was no evidence for the formation of phenolic steroids. 7. Oestrone and oestradiol-17beta were interconvertible, the reaction favouring the formation of oestrone.

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Studies on steroid metabolism in human endometrial tissue

Abstract

1. A computerized technique is described for the quantitative determination of radiometabolites from incubation studies. 2. Seven steroid substrates have been incubated with human endometrial tissue. The principal radiometabolites were identified and determined after 2hr. incubation without the addition of cofactors and after 4hr. incubation with cofactors. 3. The main products from progesterone were 20α-dihydroprogesterone and 5α-pregnanedione with lower yields of 5β-pregnanedione and 20β-dihydroprogesterone. There was no evidence for 17α-hydroxylase activity. 4. 17α-Hydroxyprogesterone was transformed into small yields of 17α,20α- and 17α,20β-dihydroxypregn-4-en-3-one. In one incubation there was evidence for conversion into androstenedione. 5. Dehydroepiandrosterone was transformed into small amounts of androstenedione, 5α-androstanedione and androsterone. 6. Androstenedione and testosterone were interconvertible, the reaction favouring the formation of androstenedione. 5α-Androstanedione and androsterone were formed from both substrates. There was no evidence for the formation of phenolic steroids. 7. Oestrone and oestradiol-17β were interconvertible, the reaction favouring the formation of oestrone.

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Selected References

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Bryson MJ, Sweat ML. Metabolism of progesterone in human proliferative endometrium. Endocrinology. 1967 Oct;81(4):729–734. [PubMed] [Google Scholar]
Collins WP, Sisterson JM, Koullapis EN, Mansfield MD, Sommerville IF. The evaluation of a gas-liquid chromatographic method for the determination of plasms testosterone using nickel-63 electron capture detection. J Chromatogr. 1968 Sep 24;37(1):33–45. [PubMed] [Google Scholar]
KREBS HA. Body size and tissue respiration. Biochim Biophys Acta. 1950 Jan;4(1-3):249–269. [PubMed] [Google Scholar]
Sweat ML, Bryson MJ, Young RB. Metabolism of 17 beta-estradiol and estrone by human proliferative endometrium and myometrium. Endocrinology. 1967 Aug;81(2):167–172. [PubMed] [Google Scholar]

Endocrine Laboratory Unit, Chelsea Hospital for Women, London S.W.3, and Institute of Obstetrics and Gynaecology, University of London

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Abstract

1. A computerized technique is described for the quantitative determination of radiometabolites from incubation studies. 2. Seven steroid substrates have been incubated with human endometrial tissue. The principal radiometabolites were identified and determined after 2hr. incubation without the addition of cofactors and after 4hr. incubation with cofactors. 3. The main products from progesterone were 20α-dihydroprogesterone and 5α-pregnanedione with lower yields of 5β-pregnanedione and 20β-dihydroprogesterone. There was no evidence for 17α-hydroxylase activity. 4. 17α-Hydroxyprogesterone was transformed into small yields of 17α,20α- and 17α,20β-dihydroxypregn-4-en-3-one. In one incubation there was evidence for conversion into androstenedione. 5. Dehydroepiandrosterone was transformed into small amounts of androstenedione, 5α-androstanedione and androsterone. 6. Androstenedione and testosterone were interconvertible, the reaction favouring the formation of androstenedione. 5α-Androstanedione and androsterone were formed from both substrates. There was no evidence for the formation of phenolic steroids. 7. Oestrone and oestradiol-17β were interconvertible, the reaction favouring the formation of oestrone.

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