Abstract:

Marked polyphenol production takes place in root tissue of sweet potato, Ipomoea batatas Lam. cv. Norin 1, in response to slicing. A possible intermediate, tentatively termed compound V, of chlorogenic acid biosynthesis was isolated from the root tissue administrated with t-cinnamic acid-2-(14)C. Compound V was proved to be an ester whose acid moiety was t-cinnamic acid, and the hydroxyl group-bearing moiety appeared to be a carbohydrate. Compound V was suggested to be the first intermediate after t-cinnamic acid involved in the chlorogenic acid biosynthetic pathway by the following three results. (a) label of t-cinnamic acid-2-(14)C was distributed in compound V first, then transferred to chlorogenic acid and isochlorogenic acid, isomers of dicaffeoylquinic acid; (b) specific radioactivity of compound V increased prior to that of the fraction containing chlorogenic acid and isochlorogenic acids and decreased prior to that of the latter; and (c) label of compound V was efficiently incorporated into chlorogenic acid and isochlorogenic acid.

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Studies on Chlorogenic Acid Biosynthesis in Sweet Potato Root Tissue in Special Reference to the Isolation of a Chlorogenic Acid Intermediate <sup><a href="#fn1" rid="fn1" class=" fn">1</a></sup>

M Kojima

I Uritani

Abstract

Marked polyphenol production takes place in root tissue of sweet potato, Ipomoea batatas Lam. cv. Norin 1, in response to slicing. A possible intermediate, tentatively termed compound V, of chlorogenic acid biosynthesis was isolated from the root tissue administrated with t-cinnamic acid-2-^{C. Compound V was proved to be an ester whose acid moiety was}t-cinnamic acid, and the hydroxyl group-bearing moiety appeared to be a carbohydrate. Compound V was suggested to be the first intermediate after t-cinnamic acid involved in the chlorogenic acid biosynthetic pathway by the following three results. (a) label of t-cinnamic acid-2-^{C was distributed in compound V first, then transferred to chlorogenic acid and isochlorogenic acid, isomers of dicaffeoylquinic acid; (}b) specific radioactivity of compound V increased prior to that of the fraction containing chlorogenic acid and isochlorogenic acids and decreased prior to that of the latter; and (c) label of compound V was efficiently incorporated into chlorogenic acid and isochlorogenic acid.

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Selected References

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GORTNER WA, KENT MJ. The coenzyme requirement and enzyme inhibitors of pineapple indoleacetic acid oxidase. J Biol Chem. 1958 Sep;233(3):731–735. [PubMed] [Google Scholar]
HANSON KR, ZUCKER M. The biosynthesis of chlorogenic acid and related conjugates of the hydroxycinnamic acids. Chromatographic separation and characterization. J Biol Chem. 1963 Mar;238:1105–1115. [PubMed] [Google Scholar]
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^{Institute for Biochemical Regulation, Faculty of Agriculture, Nagoya University, Chikusa, Nagoya, Japan 464}

^{This paper constitute Part 94 of the “Phytopathological Chemistry of Sweet Potato with Black Rot and Injury.”}

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Abstract

Marked polyphenol production takes place in root tissue of sweet potato, Ipomoea batatas Lam. cv. Norin 1, in response to slicing. A possible intermediate, tentatively termed compound V, of chlorogenic acid biosynthesis was isolated from the root tissue administrated with t-cinnamic acid-2-^{C. Compound V was proved to be an ester whose acid moiety was}t-cinnamic acid, and the hydroxyl group-bearing moiety appeared to be a carbohydrate. Compound V was suggested to be the first intermediate after t-cinnamic acid involved in the chlorogenic acid biosynthetic pathway by the following three results. (a) label of t-cinnamic acid-2-^{C was distributed in compound V first, then transferred to chlorogenic acid and isochlorogenic acid, isomers of dicaffeoylquinic acid; (}b) specific radioactivity of compound V increased prior to that of the fraction containing chlorogenic acid and isochlorogenic acids and decreased prior to that of the latter; and (c) label of compound V was efficiently incorporated into chlorogenic acid and isochlorogenic acid.

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