J Bacteriol 137(1): 692-693
Simple method for identification of plasmid-coded proteins.
Abstract
Proteins encoded by plasmid DNA are specifically labeled in UV-irradiated cells of Escherichia coli carrying recA and uvrA mutations because extensive degradation of the chromosome DNA occurs concurrently with amplification of plasmid DNA.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (474K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Images in this article
Click on the image to see a larger version.
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Benzinger R. Transfection of Enterobacteriaceae and its applications. Microbiol Rev. 1978 Mar;42(1):194–236.[PMC free article] [PubMed] [Google Scholar]
- Clewell DB. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676.[PMC free article] [PubMed] [Google Scholar]
- Howard-Flanders P. Genes that control DNA repair and genetic recombination in Escherichia coli. Adv Biol Med Phys. 1968;12:299–317. [PubMed] [Google Scholar]
- Jaskunas SR, Lindahl L, Nomura M. Identification of two copies of the gene for the elongation factor EF-Tu in E. coli. Nature. 1975 Oct 9;257(5526):458–462. [PubMed] [Google Scholar]
- Kennedy N, Beutin L, Achtman M, Skurray R, Rahmsdorf U, Herrlich P. Conjugation proteins encoded by the F sex factor. Nature. 1977 Dec 15;270(5638):580–585. [PubMed] [Google Scholar]
- Meagher RB, Tait RC, Betlach M, Boyer HW. Protein expression in E. coli minicells by recombinant plasmids. Cell. 1977 Mar;10(3):521–536. [PubMed] [Google Scholar]
- Miozzari GF, Yanofsky C. Translation of the leader region of the Escherichia coli tryptophan operon. J Bacteriol. 1978 Mar;133(3):1457–1466.[PMC free article] [PubMed] [Google Scholar]
- Ptashne M. ISOLATION OF THE lambda PHAGE REPRESSOR. Proc Natl Acad Sci U S A. 1967 Feb;57(2):306–313.[PMC free article] [PubMed] [Google Scholar]
- Sancar A, Rupert CS. Determination of plasmid molecular weights from ultraviolet sensitivities. Nature. 1978 Mar 30;272(5652):471–472. [PubMed] [Google Scholar]
- Silerman M, Matsumura P, Draper R, Edwards S, Simon MI. Expression of flagellar genes carried by bacteriophage lambda. Nature. 1976 May 20;261(5557):248–250. [PubMed] [Google Scholar]

