SIFT: predicting amino acid changes that affect protein function

Pauline Ng

Steven Henikoff

Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N A1-162, Seattle, WA 98109, USA

^{To whom correspondence should be addressed. Tel: +1 2066674515; Fax: +1 2066675889; Email:}gro.crchf@hevets

Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N A1-162, Seattle, WA 98109, USA

Received 2003 Jan 4; Revised 2003 Feb 28; Accepted 2003 Feb 28.

Abstract

Single nucleotide polymorphism (SNP) studies and random mutagenesis projects identify amino acid substitutions in protein-coding regions. Each substitution has the potential to affect protein function. SIFT (Sorting Intolerant From Tolerant) is a program that predicts whether an amino acid substitution affects protein function so that users can prioritize substitutions for further study. We have shown that SIFT can distinguish between functionally neutral and deleterious amino acid changes in mutagenesis studies and on human polymorphisms. SIFT is available at http://blocks.fhcrc.org/sift/SIFT.html.

Abstract

ACKNOWLEDGEMENTS

We thank Jorja Henikoff for advice and encouragement. This work was supported by a grant from NIH (GM29009).

ACKNOWLEDGEMENTS

REFERENCES

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