J Virol 44(3): 1039-1046
Protease bypass of temperature-sensitive murine leukemia virus maturation mutants.
Abstract
Cells infected with certain temperature-sensitive mutants of Moloney murine leukemia virus synthesize the virion precursor proteins but neither bud virions nor cleave precursor proteins to their mature form. Addition of proteases to cells infected with these mutants caused cleavage of the precursor proteins Pr65gag and Pr180gag-pol to their mature forms at the nonpermissive temperature. Concomitantly there was release from the cells of morphologically normal virions. The enzymatically inactive Pr180gag-pol was cleaved to active reverse transcriptase (p85), which was found in the released particles. External protease treatment apparently bypassed the lesion in these viral mutants, suggesting that their defect may involve a virus-specific protease.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bonner WM, Laskey RA. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. [PubMed] [Google Scholar]
- Demsey A, Kawka D, Galuska S, Stackpole CW. Assembly of a temperature-sensitive mutant of Rauscher murine leukemia virus at the cell surface induced by low temperature and by ligands. Virology. 1979 May;95(1):235–240. [PubMed] [Google Scholar]
- Dittmar KJ, Moelling K. Biochemical properties of p15-associated protease in an avian RNA tumor virus. J Virol. 1978 Oct;28(1):106–118.[PMC free article] [PubMed] [Google Scholar]
- Kessler SW. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
- Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed] [Google Scholar]
- Lu AH, Soong MM, Wong PK. Maturation of Moloney murine leukemia virus. Virology. 1979 Feb;93(1):269–274. [PubMed] [Google Scholar]
- Paskind MP, Weinberg RA, Baltimore D. Dependence of Moloney murine leukemia virus production on cell growth. Virology. 1975 Sep;67(1):242–248. [PubMed] [Google Scholar]
- Rohrschneider JM, Diggelmann H, Ogura H, Friis RR, Bauer H. Defective cleavage of a precursor polypeptide in a temperature-sensitive mutant of avian sarcoma virus. Virology. 1976 Nov;75(1):177–187. [PubMed] [Google Scholar]
- Rowe WP, Pugh WE, Hartley JW. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. [PubMed] [Google Scholar]
- Stephenson JR, Aaronson SA. Characterization of temperature-sensitive mutants of murine leukemia virus. Virology. 1973 Jul;54(1):53–59. [PubMed] [Google Scholar]
- van de Ven WJ, van Zaane D, Onnekink C, Bloemers HP. Impaired processing of precursor polypeptides of temperature-sensitive mutants of Rauscher murine leukemia virus. J Virol. 1978 Feb;25(2):553–561.[PMC free article] [PubMed] [Google Scholar]
- von der Helm K. Cleavage of Rous sarcoma viral polypeptide precursor into internal structural proteins in vitro involves viral protein p15. Proc Natl Acad Sci U S A. 1977 Mar;74(3):911–915.[PMC free article] [PubMed] [Google Scholar]
- Wallach D, Anderson W, Pastan I. Activation of adenylate cyclase in cultured fibroblasts by trypsin. J Biol Chem. 1978 Jan 10;253(1):24–26. [PubMed] [Google Scholar]
- Werb Z, Aggeler J. Proteases induce secretion of collagenase and plasminogen activator by fibroblasts. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1839–1843.[PMC free article] [PubMed] [Google Scholar]
- Witte ON, Baltimore D. Relationship of retrovirus polyprotein cleavages to virion maturation studied with temperature-sensitive murine leukemia virus mutants. J Virol. 1978 Jun;26(3):750–761.[PMC free article] [PubMed] [Google Scholar]
- Wong PK, MacLeod R. Studies on the budding process of a temperature-sensitive mutant of murine leukemia virus with a scanning electron microscope. J Virol. 1975 Aug;16(2):434–442.[PMC free article] [PubMed] [Google Scholar]
- Wong PK, McCarter JA. Studies of two temperature-sensitive mutants of Moloney murine leukemia virus. Virology. 1974 Apr;58(2):396–408. [PubMed] [Google Scholar]
- Yeger H, Kalnins VI, Stephenson JR. Type-C retrovirus maturation and assembly: post-translational cleavage of the gag-gene coded precursor polypeptide occurs at the cell membrane. Virology. 1978 Aug;89(1):34–44. [PubMed] [Google Scholar]
- Yoshinaka Y, Ishigame K, Ohno T, Kageyama S, Shibata K, Luftig RB. Preparations enriched for "immature" murine leukemia virus particles that remain in tissue culture fluids are deficient in Pr65gag proteolyic activity. Virology. 1980 Jan 15;100(1):130–140. [PubMed] [Google Scholar]
- Yoshinaka Y, Luftig RB. Properties of a P70 proteolytic factor of murine leukemia viruses. Cell. 1977 Nov;12(3):709–719. [PubMed] [Google Scholar]
- Yoshinaka Y, Luftig RB. Morphological conversion of 'immature' Rauscher leukaemia virus cores to a 'mature' form after addition of the P65-70 (gag gene product) proteolytic factor. J Gen Virol. 1978 Jul;40(1):151–160. [PubMed] [Google Scholar]