Properties of crude Campylobacter jejuni heat-labile enterotoxin.
PDF (1.1M)
Journal: 1984/September - Infection and Immunity
ISSN: 0019-9567
PUBMED: 6746090
Abstract:
The amount of crude Campylobacter jejuni enterotoxin present in culture products was quantitated by comparing the response of these preparations with that of pure Escherichia coli heat-labile toxin (LT) in the Chinese hamster ovary assay and in enzyme-linked immunosorbent assays that used GM ganglioside or antisera to LT or both. Maximum C. jejuni enterotoxin production was achieved by growth at 42 degrees C for 24 h under agitation in supplemented GC medium. Adding polymyxin separately to either the broth supernatant or the cells enhanced the recovery of toxin; the yield from cell lysates was much lower. The quantity of C. jejuni enterotoxin produced by clinical isolates obtained locally or provided from Mexico varied widely, over a spectrum from none to large amounts; quantitative values for the amount of C. jejuni enterotoxin determined by the Chinese hamster ovary and enzyme-linked immunosorbent assays correlated with the degree of secretory potency of this material in ligated rat ileal loops. The cytotonic activity of C. jejuni enterotoxin in Chinese hamster ovary cells was abolished by heating at 96 degrees C for 10 min and by preincubation either with GM ganglioside or with LT or cholera toxin antisera. The secretory activity of C. jejuni enterotoxin in ligated rat ileal loops was passively neutralized by antiserum to LT, and immunizing rats with either LT or its B subunit significantly (P less than 0.001) reduced fluid response to active challenge with C. jejuni enterotoxin in ligated ileal loops. These observations indicate that strains of C. jejuni vary in their capacity to elaborate a heat-labile enterotoxin that has close immunological homology with LT and cholera toxin.
Open in
PUBMED | PMC | Google Scholar | Wikipedia
Relations:
Content
Citations
(24)
References
(47)
Drugs
(1)
Chemicals
(5)
Organisms
(4)
Processes
(1)
Similar articles
Articles by the same authors
Discussion board
Infect Immun 45(2): 314-319
PMID: 6746090

Properties of crude Campylobacter jejuni heat-labile enterotoxin.

Abstract

The amount of crude Campylobacter jejuni enterotoxin present in culture products was quantitated by comparing the response of these preparations with that of pure Escherichia coli heat-labile toxin (LT) in the Chinese hamster ovary assay and in enzyme-linked immunosorbent assays that used GM ganglioside or antisera to LT or both. Maximum C. jejuni enterotoxin production was achieved by growth at 42 degrees C for 24 h under agitation in supplemented GC medium. Adding polymyxin separately to either the broth supernatant or the cells enhanced the recovery of toxin; the yield from cell lysates was much lower. The quantity of C. jejuni enterotoxin produced by clinical isolates obtained locally or provided from Mexico varied widely, over a spectrum from none to large amounts; quantitative values for the amount of C. jejuni enterotoxin determined by the Chinese hamster ovary and enzyme-linked immunosorbent assays correlated with the degree of secretory potency of this material in ligated rat ileal loops. The cytotonic activity of C. jejuni enterotoxin in Chinese hamster ovary cells was abolished by heating at 96 degrees C for 10 min and by preincubation either with GM ganglioside or with LT or cholera toxin antisera. The secretory activity of C. jejuni enterotoxin in ligated rat ileal loops was passively neutralized by antiserum to LT, and immunizing rats with either LT or its B subunit significantly (P less than 0.001) reduced fluid response to active challenge with C. jejuni enterotoxin in ligated ileal loops. These observations indicate that strains of C. jejuni vary in their capacity to elaborate a heat-labile enterotoxin that has close immunological homology with LT and cholera toxin.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
icon of scanned page 314
314
icon of scanned page 315
315
icon of scanned page 316
316
icon of scanned page 317
317
icon of scanned page 318
318
icon of scanned page 319
319

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Black RE, Merson MH, Huq I, Alim AR, Yunus M. Incidence and severity of rotavirus and Escherichia coli diarrhoea in rural Bangladesh. Implications for vaccine development. Lancet. 1981 Jan 17;1(8212):141–143. [PubMed] [Google Scholar]
  • Blaser MJ, Berkowitz ID, LaForce FM, Cravens J, Reller LB, Wang WL. Campylobacter enteritis: clinical and epidemiologic features. Ann Intern Med. 1979 Aug;91(2):179–185. [PubMed] [Google Scholar]
  • Blaser MJ, Glass RI, Huq MI, Stoll B, Kibriya GM, Alim AR. Isolation of Campylobacter fetus subsp. jejuni from Bangladeshi children. J Clin Microbiol. 1980 Dec;12(6):744–747.[PMC free article] [PubMed] [Google Scholar]
  • Blaser MJ, Reller LB. Campylobacter enteritis. N Engl J Med. 1981 Dec 10;305(24):1444–1452. [PubMed] [Google Scholar]
  • Bokkenheuser VD, Richardson NJ, Bryner JH, Roux DJ, Schutte AB, Koornhof HJ, Freiman I, Hartman E. Detection of enteric campylobacteriosis in children. J Clin Microbiol. 1979 Feb;9(2):227–232.[PMC free article] [PubMed] [Google Scholar]
  • Branski D, Roll D, Gross-Kieselstein E, Gross V, Greenberg L, Abrahamov A. Campylobacter enteritis in infants and young children. Isr J Med Sci. 1982 May;18(5):615–617. [PubMed] [Google Scholar]
  • Caldwell MB, Walker RI, Stewart SD, Rogers JE. Simple adult rabbit model for Campylobacter jejuni enteritis. Infect Immun. 1983 Dec;42(3):1176–1182.[PMC free article] [PubMed] [Google Scholar]
  • Caprioli A, Falbo V, Roda LG, Ruggeri FM, Zona C. Partial purification and characterization of an escherichia coli toxic factor that induces morphological cell alterations. Infect Immun. 1983 Mar;39(3):1300–1306.[PMC free article] [PubMed] [Google Scholar]
  • Clements JD, Finkelstein RA. Isolation and characterization of homogeneous heat-labile enterotoxins with high specific activity from Escherichia coli cultures. Infect Immun. 1979 Jun;24(3):760–769.[PMC free article] [PubMed] [Google Scholar]
  • Clements JD, Flint DC, Engert RF, Klipstein FA. Cloning and molecular characterization of the B subunit of Escherichia coli heat-labile enterotoxin. Infect Immun. 1983 May;40(2):653–658.[PMC free article] [PubMed] [Google Scholar]
  • Clements JD, Flint DC, Klipstein FA. Immunological and physicochemical characterization of heat-labile enterotoxins isolated from two strains of Escherichia coli. Infect Immun. 1982 Nov;38(2):806–809.[PMC free article] [PubMed] [Google Scholar]
  • Clements JD, Yancey RJ, Finkelstein RA. Properties of homogeneous heat-labile enterotoxin from Escherichia coli. Infect Immun. 1980 Jul;29(1):91–97.[PMC free article] [PubMed] [Google Scholar]
  • De Mol P, Bosmans E. Campylobacter enteritis in Central Africa. Lancet. 1978 Mar 18;1(8064):604–604. [PubMed] [Google Scholar]
  • Donta ST, Viner JP. Inhibition of the steroidogenic effects of cholera and heat-labile Escherichia coli enterotoxins by GM1 ganglioside: evidence for a similar receptor site for the two toxins. Infect Immun. 1975 May;11(5):982–985.[PMC free article] [PubMed] [Google Scholar]
  • Drake AA, Gilchrist MJ, Washington JA, 2nd, Huizenga KA, Van Scoy RE. Diarrhea due to Campylobacter fetus subspecies jejuni. A clinical review of 63 cases. Mayo Clin Proc. 1981 Jul;56(7):414–423. [PubMed] [Google Scholar]
  • Fernández H, Neto UF, Fernandes F, de Almeida Pedra M, Trabulsi LR. Culture supernatants of Campylobacter jejuni induce a secretory response in jejunal segments of adult rats. Infect Immun. 1983 Apr;40(1):429–431.[PMC free article] [PubMed] [Google Scholar]
  • Glass RI, Stoll BJ, Huq MI, Struelens MJ, Blaser M, Kibriya AK. Epidemiologic and clinical features of endemic Campylobacter jejuni infection in Bangladesh. J Infect Dis. 1983 Aug;148(2):292–296. [PubMed] [Google Scholar]
  • Greenberg HB, Sack DA, Rodriguez W, Sack RB, Wyatt RG, Kalica AR, Horswood RL, Chanock RM, Kapikian AZ. Microtiter solid-phase radioimmunoassay for detection of Escherichia coli heat-labile enterotoxin. Infect Immun. 1977 Sep;17(3):541–545.[PMC free article] [PubMed] [Google Scholar]
  • Guerrant RL, Brunton LL, Schnaitman TC, Rebhun LI, Gilman AG. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infect Immun. 1974 Aug;10(2):320–327.[PMC free article] [PubMed] [Google Scholar]
  • Harris JR, Wachsmuth IK, Davis BR, Cohen ML. High-molecular-weight plasmid correlates with Escherichia coli enteroinvasiveness. Infect Immun. 1982 Sep;37(3):1295–1298.[PMC free article] [PubMed] [Google Scholar]
  • Johnson WM, Lior H. Toxins produced by Campylobacter jejuni and Campylobacter coli. Lancet. 1984 Jan 28;1(8370):229–230. [PubMed] [Google Scholar]
  • Kantor HS, Tao P, Gorbach SL. Stimulation of intestinal adenyl cyclase by Escherichia coli enterotoxin: comparison of strains from an infant and an adult with diarrhea. J Infect Dis. 1974 Jan;129(1):1–9. [PubMed] [Google Scholar]
  • Kapikian AZ, Wyatt RG, Greenberg HB, Kalica AR, Kim HW, Brandt CD, Rodriguez WJ, Parrott RH, Chanock RM. Approaches to immunization of infants and young children against gastroenteritis due to rotaviruses. Rev Infect Dis. 1980 May-Jun;2(3):459–469. [PubMed] [Google Scholar]
  • Klipstein FA, Engert RF. Protective effect of immunization of rats with holotoxin or B subunit of Escherichia coli heat-labile enterotoxin. Infect Immun. 1981 Jan;31(1):144–150.[PMC free article] [PubMed] [Google Scholar]
  • Klipstein FA, Engert RF, Clements JD, Houghten RA. Vaccine for enterotoxigenic Escherichia coli based on synthetic heat-stable toxin crossed-linked to the B subunit of heat-labile toxin. J Infect Dis. 1983 Feb;147(2):318–326. [PubMed] [Google Scholar]
  • Klipstein FA, Engert RF, Houghten RA. Protection in rabbits immunized with a vaccine of Escherichia coli heat-stable toxin cross-linked to the heat-labile toxin B subunit. Infect Immun. 1983 Jun;40(3):888–893.[PMC free article] [PubMed] [Google Scholar]
  • Klipstein FA, Engert RF, Houghten RA, Rowe B. Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin. J Clin Microbiol. 1984 Jun;19(6):798–803.[PMC free article] [PubMed] [Google Scholar]
  • Lambert ME, Schofield PF, Ironside AG, Mandal BK. Campylobacter colitis. Br Med J. 1979 Mar 31;1(6167):857–859.[PMC free article] [PubMed] [Google Scholar]
  • Levine MM, Kaper JB, Black RE, Clements ML. New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development. Microbiol Rev. 1983 Dec;47(4):510–550.[PMC free article] [PubMed] [Google Scholar]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  • Manninen KI, Prescott JF, Dohoo IR. Pathogenicity of Campylobacter jejuni isolates from animals and humans. Infect Immun. 1982 Oct;38(1):46–52.[PMC free article] [PubMed] [Google Scholar]
  • Osborne JC, Moss J, Fishman PH, Nakaya S, Robertson DC. Specificity in protein-membrane associations: the interaction of gangliosides with Escherichia coli heat-labile enterotoxin and choleragen. Biophys J. 1982 Jan;37(1):168–169.[PMC free article] [PubMed] [Google Scholar]
  • Pai CH, Sorger S, Lackman L, Sinai RE, Marks MI. Campylobacter gastroenteritis in children. J Pediatr. 1979 Apr;94(4):589–591. [PubMed] [Google Scholar]
  • Peterson JW. Salmonella toxin. Pharmacol Ther. 1980;11(3):719–724. [PubMed] [Google Scholar]
  • Peterson JW, Sandefur PD. Evidence of a role for permeability factors in the pathogenesis of salmonellosis. Am J Clin Nutr. 1979 Jan;32(1):197–209. [PubMed] [Google Scholar]
  • Pitkänen T. Travellers' diarrhoea caused by Campylobacter jejuni. Ann Clin Res. 1982 Apr;14(2):111–113. [PubMed] [Google Scholar]
  • Rajan DP, Mathan VI. Prevalence of Campylobacter fetus subsp. jejuni in healthy populations in southern India. J Clin Microbiol. 1982 May;15(5):749–751.[PMC free article] [PubMed] [Google Scholar]
  • Rowe B. The role of Escherichia coli in gastroenteritis. Clin Gastroenterol. 1979 Sep;8(3):625–644. [PubMed] [Google Scholar]
  • Ruiz-Palacios GM, Torres J, Torres NI, Escamilla E, Ruiz-Palacios BR, Tamayo J. Cholera-like enterotoxin produced by Campylobacter jejuni. Characterisation and clinical significance. Lancet. 1983 Jul 30;2(8344):250–253. [PubMed] [Google Scholar]
  • Ryder RW, Oquist CA, Greenberg H, Taylor DN, Orskov F, Orskov I, Kapikian AZ, Sack RB. Travelers' diarrhea in panamanian tourists in Mexico. J Infect Dis. 1981 Nov;144(5):442–448. [PubMed] [Google Scholar]
  • Ryder RW, Sack DA, Kapikian AZ, McLaughlin JC, Chakraborty J, Mizanur Rahman AS, Merson MH, Wells JG. Enterotoxigenic Escherichia coli and Reovirus-like agent in rural Bangladesh. Lancet. 1976 Mar 27;1(7961):659–663. [PubMed] [Google Scholar]
  • Sandefur PD, Peterson JW. Neutralization of Salmonella toxin-induced elongation of Chinese hamster ovary cells by cholera antitoxin. Infect Immun. 1977 Mar;15(3):988–992.[PMC free article] [PubMed] [Google Scholar]
  • Smith HR, Scotland SM, Rowe B. Plasmids that code for production of colonization factor antigen II and enterotoxin production in strains of Escherichia coli. Infect Immun. 1983 Jun;40(3):1236–1239.[PMC free article] [PubMed] [Google Scholar]
  • Snyder JD, Merson MH. The magnitude of the global problem of acute diarrhoeal disease: a review of active surveillance data. Bull World Health Organ. 1982;60(4):605–613.[PMC free article] [PubMed] [Google Scholar]
  • Speelman P, Struelens MJ, Sanyal SC, Glass RI. Detection of Campylobacter jejuni and other potential pathogens in travellers' diarrhoea in Bangladesh. Scand J Gastroenterol Suppl. 1983;84:19–23. [PubMed] [Google Scholar]
  • Svedhem A, Kaijser B. Campylobacter fetus subspecies jejuni: a common cause of diarrhea in Sweden. J Infect Dis. 1980 Sep;142(3):353–359. [PubMed] [Google Scholar]
  • Wadström T, Baloda SB, Krovacek K, Faris A, Bengtson S, Walder M. Swedish isolates of Campylobacter jejuni/coli do not produce cytotonic or cytotoxic enterotoxins. Lancet. 1983 Oct 15;2(8355):911–911. [PubMed] [Google Scholar]
Copyright notice
Abstract
The amount of crude Campylobacter jejuni enterotoxin present in culture products was quantitated by comparing the response of these preparations with that of pure Escherichia coli heat-labile toxin (LT) in the Chinese hamster ovary assay and in enzyme-linked immunosorbent assays that used GM ganglioside or antisera to LT or both. Maximum C. jejuni enterotoxin production was achieved by growth at 42 degrees C for 24 h under agitation in supplemented GC medium. Adding polymyxin separately to either the broth supernatant or the cells enhanced the recovery of toxin; the yield from cell lysates was much lower. The quantity of C. jejuni enterotoxin produced by clinical isolates obtained locally or provided from Mexico varied widely, over a spectrum from none to large amounts; quantitative values for the amount of C. jejuni enterotoxin determined by the Chinese hamster ovary and enzyme-linked immunosorbent assays correlated with the degree of secretory potency of this material in ligated rat ileal loops. The cytotonic activity of C. jejuni enterotoxin in Chinese hamster ovary cells was abolished by heating at 96 degrees C for 10 min and by preincubation either with GM ganglioside or with LT or cholera toxin antisera. The secretory activity of C. jejuni enterotoxin in ligated rat ileal loops was passively neutralized by antiserum to LT, and immunizing rats with either LT or its B subunit significantly (P less than 0.001) reduced fluid response to active challenge with C. jejuni enterotoxin in ligated ileal loops. These observations indicate that strains of C. jejuni vary in their capacity to elaborate a heat-labile enterotoxin that has close immunological homology with LT and cholera toxin.
Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.