Polyadenylic acid at the 3'-terminus of poliovirus RNA.
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Journal: 1972/September - Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
PUBMED: 4340162
Abstract:
Poliovirus RNA that has been derivatized at the 3'-end with NaIO(4)-NaB(3)H(4) yields, after hydrolysis with alkali or RNase T2, predominantly labeled residues of modified adenosine; no labeled nucleoside derivative is produced by digestion with RNase A or RNase T1. The 3'-terminal bases of the RNA are, therefore,...ApA(OH). Hydrolyzates of poliovirus [(32)P]RNA, after exhaustive digestion with RNase T1 or RNase A, contain, besides internal oligonucleotides, polynucleotides resistant to further action of ribonucleases T1 and A, respectively; these polynucleotides were isolated by membrane-filter binding or ion-exchange chromatography. The sequence of the T1-resistant polynucleotide was determined to be (Ap)(n)A(OH), that of the RNase A-resistant polynucleotide was GpGp(Ap)(n)A(OH). The chain length (n) of the polyadenylic acid, as analyzed by different methods, averages 89 nucleotides. Gel electrophoresis revealed heterogeneity of the size of poly(A). Poliovirus RNA, when labeled in vitro at the 3'-end, contains [3'-(3)H]poly(A); when labeled in vivo with [(3)H]A, it contains [(3)H](Ap)(n)A(OH). The data establish that... YpGpGp(Ap)([unk])A(OH) is the 3'-terminal sequence of poliovirus RNA, Type 1 (Mahoney). Since this mammalian virus reproduces in the cell cytoplasm, these observations may modify prior interpretations of the function of polyadenylate ends on messenger RNAs.
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Proc Natl Acad Sci U S A 69(7): 1877-1882
PMID: 4340162

Polyadenylic Acid at the 3′-Terminus of Poliovirus RNA<sup><a href="#fn1" rid="fn1" class=" fn">*</a></sup>

Abstract

Poliovirus RNA that has been derivatized at the 3′-end with NaIO4-NaBH4 yields, after hydrolysis with alkali or RNase T2, predominantly labeled residues of modified adenosine; no labeled nucleoside derivative is produced by digestion with RNase A or RNase T1. The 3′-terminal bases of the RNA are, therefore,...ApAOH. Hydrolyzates of poliovirus [P]RNA, after exhaustive digestion with RNase T1 or RNase A, contain, besides internal oligonucleotides, polynucleotides resistant to further action of ribonucleases T1 and A, respectively; these polynucleotides were isolated by membrane-filter binding or ion-exchange chromatography. The sequence of the T1-resistant polynucleotide was determined to be (Ap)nAOH, that of the RNase A-resistant polynucleotide was GpGp(Ap)nAOH. The chain length (n) of the polyadenylic acid, as analyzed by different methods, averages 89 nucleotides. Gel electrophoresis revealed heterogeneity of the size of poly(A). Poliovirus RNA, when labeled in vitro at the 3′-end, contains [3′-H]poly(A); when labeled in vivo with [H]A, it contains [H](Ap)nAOH. The data establish that... YpGpGp(Ap)[unk]AOH is the 3′-terminal sequence of poliovirus RNA, Type 1 (Mahoney). Since this mammalian virus reproduces in the cell cytoplasm, these observations may modify prior interpretations of the function of polyadenylate ends on messenger RNAs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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Department of Microbiology, St. Louis University School of Medicine, St. Louis, Missouri 63104
This is paper II in the series, “Sequence Studies of Poliovirus RNA.” An abstract of this work appeared in Bacteriol. Proc. 236 (1972).
Copyright notice
Abstract
Poliovirus RNA that has been derivatized at the 3′-end with NaIO4-NaBH4 yields, after hydrolysis with alkali or RNase T2, predominantly labeled residues of modified adenosine; no labeled nucleoside derivative is produced by digestion with RNase A or RNase T1. The 3′-terminal bases of the RNA are, therefore,...ApAOH. Hydrolyzates of poliovirus [P]RNA, after exhaustive digestion with RNase T1 or RNase A, contain, besides internal oligonucleotides, polynucleotides resistant to further action of ribonucleases T1 and A, respectively; these polynucleotides were isolated by membrane-filter binding or ion-exchange chromatography. The sequence of the T1-resistant polynucleotide was determined to be (Ap)nAOH, that of the RNase A-resistant polynucleotide was GpGp(Ap)nAOH. The chain length (n) of the polyadenylic acid, as analyzed by different methods, averages 89 nucleotides. Gel electrophoresis revealed heterogeneity of the size of poly(A). Poliovirus RNA, when labeled in vitro at the 3′-end, contains [3′-H]poly(A); when labeled in vivo with [H]A, it contains [H](Ap)nAOH. The data establish that... YpGpGp(Ap)[unk]AOH is the 3′-terminal sequence of poliovirus RNA, Type 1 (Mahoney). Since this mammalian virus reproduces in the cell cytoplasm, these observations may modify prior interpretations of the function of polyadenylate ends on messenger RNAs.
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