Abstract:

Investigation of polyphenol production in cut-injured sweet potato (Ipomoea batatas Lam. cv. Kokei 14) roots by histochemical and quantitative methods showed that polyphenols were produced in striking amounts in the proximal side of the tissue pieces (2 cm thick), but only in small amounts in cells of the distal side. In response to cut injury, formation of the enzymes related to polyphenol biosynthesis, phenylalanine ammonia-lyase and trans-cinnamic acid 4-hydroxylase, was also pronounced in the proximal side of the tissue pieces and slight in the distal side. The similar polarity was observed in the development of activities of various enzymes, such as NADPH-cytochrome c oxidoreductase, acid invertase, peroxidase, o-diphenol oxidase, and cytochrome c-O(2) oxidoreductase. Acropetal development of polyphenol contents and of various enzyme activities may be related to the acropetal movement of indoleacetic acid (IAA) in roots of various plants. Treatment of the distal surface of tissue pieces with IAA or 2,4-dichlorophenoxyacetic acid caused polyphenol production but treatment with gibberellic acid, abscisic acid, kinetin, or ethylene had little effect. The results suggest that IAA may play a role in the metabolic response to cut injury.

Open in

PUBMED | PMC | Google Scholar | Wikipedia

Relations:

Content

Citations

(3)

References

(8)

Drugs

(3)

Affiliates

(1)

Polarity of Production of Polyphenols and Development of Various Enzyme Activities in Cut-injured Sweet Potato Root Tissue <sup><a href="#fn1" rid="fn1" class=" fn">1</a></sup>

Y Tanaka

I Uritani

Abstract

Investigation of polyphenol production in cut-injured sweet potato (Ipomoea batatas Lam. cv. Kokei 14) roots by histochemical and quantitative methods showed that polyphenols were produced in striking amounts in the proximal side of the tissue pieces (2 cm thick), but only in small amounts in cells of the distal side. In response to cut injury, formation of the enzymes related to polyphenol biosynthesis, phenylalanine ammonia-lyase and trans-cinnamic acid 4-hydroxylase, was also pronounced in the proximal side of the tissue pieces and slight in the distal side. The similar polarity was observed in the development of activities of various enzymes, such as NADPH-cytochrome c oxidoreductase, acid invertase, peroxidase, o-diphenol oxidase, and cytochrome c-O₂ oxidoreductase. Acropetal development of polyphenol contents and of various enzyme activities may be related to the acropetal movement of indoleacetic acid (IAA) in roots of various plants. Treatment of the distal surface of tissue pieces with IAA or 2,4-dichlorophenoxyacetic acid caused polyphenol production but treatment with gibberellic acid, abscisic acid, kinetin, or ethylene had little effect. The results suggest that IAA may play a role in the metabolic response to cut injury.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (764K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Image
on p.564

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

Hyodo H, Uritani I. Properties of polyphenol oxidases produced in sweet potato tissue after wounding. Arch Biochem Biophys. 1967 Nov;122(2):299–309. [PubMed] [Google Scholar]
LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
Nakamura K, Asahi T. Changes in properties of the inner mitochondrial membrane during mitochondrial biogenesis in aging sweet potato tissue slices in relation to the development of cyanide-insensitive respiration. Arch Biochem Biophys. 1976 Jun;174(2):393–401. [PubMed] [Google Scholar]
REEVE RM. Histochemical tests for polyphenols in plant tissues. Stain Technol. 1951 Apr;26(2):91–96. [PubMed] [Google Scholar]
Tanaka Y, Uritani I. Immunochemical studies on fluctuation of phenylalanine ammonia-lyase activity in sweet potato in response to cut injury. J Biochem. 1976 Jan;79(1):217–219. [PubMed] [Google Scholar]
Tanaka Y, Uritani I. Synthesis and turnover of phenylalanine ammonia-lyase in root tissue of sweet potatoe injured by cutting. Eur J Biochem. 1977 Feb 15;73(1):255–260. [PubMed] [Google Scholar]
Watanabe A, Imaseki H. Induction of deoxyribonucleic Acid synthesis in potato tuber tissue by cutting. Plant Physiol. 1973 Apr;51(4):772–776.[PMC free article] [PubMed] [Google Scholar]
Wilkins MB, Scott TK. Auxin transport in roots. Nature. 1968 Sep 28;219(5161):1388–1389. [PubMed] [Google Scholar]

^{Laboratory of Biochemistry, Faculty of Agriculture, Nagoya University, Chikusa, Nagoya 464, Japan}

^{This work was supported in part by a grant from the Ministry of Education.}

Copyright notice

Abstract

Investigation of polyphenol production in cut-injured sweet potato (Ipomoea batatas Lam. cv. Kokei 14) roots by histochemical and quantitative methods showed that polyphenols were produced in striking amounts in the proximal side of the tissue pieces (2 cm thick), but only in small amounts in cells of the distal side. In response to cut injury, formation of the enzymes related to polyphenol biosynthesis, phenylalanine ammonia-lyase and trans-cinnamic acid 4-hydroxylase, was also pronounced in the proximal side of the tissue pieces and slight in the distal side. The similar polarity was observed in the development of activities of various enzymes, such as NADPH-cytochrome c oxidoreductase, acid invertase, peroxidase, o-diphenol oxidase, and cytochrome c-O₂ oxidoreductase. Acropetal development of polyphenol contents and of various enzyme activities may be related to the acropetal movement of indoleacetic acid (IAA) in roots of various plants. Treatment of the distal surface of tissue pieces with IAA or 2,4-dichlorophenoxyacetic acid caused polyphenol production but treatment with gibberellic acid, abscisic acid, kinetin, or ethylene had little effect. The results suggest that IAA may play a role in the metabolic response to cut injury.

Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.

Learn More