Proc Natl Acad Sci U S A 74(8): 3259-3263
Packaging recombinant DNA molecules into bacteriophage particles in vitro.
Abstract
Recombinant phage genomes made in reactions with purified enzymes may be recovered directly by packaging into phage heads in vitro. The process is efficient and nonselective and offers containment in initial stages of handling recombinant DNA. Ligase [poly(deoxyribonucleotide):poly-(deoxyribonucleotide) ligase (AMP-forming), EC 6.5.1.1] reaction products can recombine with endogenous phage DNA during packaging, but UV-irradiation eliminates the biological activity of the endogenous DNA.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chang AC, Cohen SN. Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1030–1034.[PMC free article] [PubMed] [Google Scholar]
- Morrow JF, Cohen SN, Chang AC, Boyer HW, Goodman HM, Helling RB. Replication and transcription of eukaryotic DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1974 May;71(5):1743–1747.[PMC free article] [PubMed] [Google Scholar]
- Kedes LH, Chang AC, Houseman D, Cohen SN. Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli. Nature. 1975 Jun 12;255(5509):533–538. [PubMed] [Google Scholar]
- Chang AC, Lansman RA, Clayton DA, Cohen SN. Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids. Cell. 1975 Oct;6(2):231–244. [PubMed] [Google Scholar]
- Murray NE, Murray K. Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments. Nature. 1974 Oct 11;251(5475):476–481. [PubMed] [Google Scholar]
- Borck K, Beggs JD, Brammar WJ, Hopkins AS, Murray NE. The construction in vitro of transducing derivatives of phage lambda. Mol Gen Genet. 1976 Jul 23;146(2):199–207. [PubMed] [Google Scholar]
- Struhl K, Cameron JR, Davis RW. Functional genetic expression of eukaryotic DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1976 May;73(5):1471–1475.[PMC free article] [PubMed] [Google Scholar]
- Ratzkin B, Carbon J. Functional expression of cloned yeast DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1977 Feb;74(2):487–491.[PMC free article] [PubMed] [Google Scholar]
- Murray NE, Brammar WJ, Murray K. Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet. 1977 Jan 7;150(1):53–61. [PubMed] [Google Scholar]
- Lederberg EM, Cohen SN. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol. 1974 Sep;119(3):1072–1074.[PMC free article] [PubMed] [Google Scholar]
- Mandel M, Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. [PubMed] [Google Scholar]
- Hohn T, Wurtz M, Hohn B. Capsid transformation during packaging of bacteriophage lambdaDNA. Philos Trans R Soc Lond B Biol Sci. 1976 Nov 30;276(943):51–61. [PubMed] [Google Scholar]
- Hohn B. DNA as substrate for packaging into bacteriophage lambda, in vitro. J Mol Biol. 1975 Oct 15;98(1):93–106. [PubMed] [Google Scholar]
- Hohn B, Hohn T. Activity of empty, headlike particles for packaging of DNA of bacteriophage lambda in vitro. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2372–2376.[PMC free article] [PubMed] [Google Scholar]
- Casjens S, Horn T, Kaiser AD. Head assembly steps controlled by genes F and W in bacteriophage lambda. J Mol Biol. 1972 Mar 14;64(3):551–563. [PubMed] [Google Scholar]
- Syvanen M. In vitro genetic recombination of bacteriophage lambda. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2496–2499.[PMC free article] [PubMed] [Google Scholar]
- Drabkina LE, Konevega LV, Legina OK, Mosevitsky MI. Dependence of transfection efficiency of calcium treated Escherichia coli cells on bacterial genotype and form of Lambda DNA. Mol Gen Genet. 1976 Feb 27;144(1):83–86. [PubMed] [Google Scholar]
- Murray K, Murray NE. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. J Mol Biol. 1975 Nov 5;98(3):551–564. [PubMed] [Google Scholar]
- Nathans D, Smith HO. Restriction endonucleases in the analysis and restructuring of dna molecules. Annu Rev Biochem. 1975;44:273–293. [PubMed] [Google Scholar]
- Sharp PA, Sugden B, Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. [PubMed] [Google Scholar]
- Weiss B, Richardson CC. Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage. Proc Natl Acad Sci U S A. 1967 Apr;57(4):1021–1028.[PMC free article] [PubMed] [Google Scholar]
- Hohn T, Flick H, Hohn B. Petit lambda, a family of particles from coliphage lambda infected cells. J Mol Biol. 1975 Oct 15;98(1):107–120. [PubMed] [Google Scholar]
- Cameron JR, Panasenko SM, Lehman IR, Davis RW. In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3416–3420.[PMC free article] [PubMed] [Google Scholar]
- Sternberg N, Tiemeier D, Enquist L. In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1. Gene. 1977 May;1(3-4):255–280. [PubMed] [Google Scholar]
- Nash HA. Integrative recombination of bacteriophage lambda DNA in vitro. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1072–1076.[PMC free article] [PubMed] [Google Scholar]
- Gottesman S, Gottesman M. Excision of prophage lambda in a cell-free system. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2188–2192.[PMC free article] [PubMed] [Google Scholar]
- Weissmann C, Boll W. Reduction of possible hazards in the preparation of recombinant plasmid DNA. Nature. 1976 Jun 3;261(5559):428–429. [PubMed] [Google Scholar]
- Emmons SW, MacCosham V, Baldwin RL. Tandem genetic duplications in phage lambda. III. The frequency of duplication mutants in two derivatives of phage lambda is independent of known recombination systems. J Mol Biol. 1975 Jan 15;91(2):133–146. [PubMed] [Google Scholar]