Abstract:

The paramagnetic resonance spectra of N-oxyl-4','-dimethyloxazolidine derivatives of 12-ketostearic acid, 5-ketostearic acid, 5-ketotricosanoic acid, and 5alpha-androstan-3-one-17beta-ol have been observed in sonicated phospholipid dispersions, the walking-leg nerve fibers of Homarus americanus, and in erythrocytes oriented by hydrodynamic shear. The preferred orientation of these spin labels in the nerve and erythrocyte membranes is one in which the long amphiphilic axis is perpendicular to the membrane surface. The resonance spectra indicate that these labels in the nerve fiber are bound to a lipid bilayer very similar to that found in the sonicated phospholipid dispersion, whereas any lipid bilayer in the erythrocyte must be much more rigid.

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ORIENTATION AND MOTION OF AMPHIPHILIC SPIN LABELS IN MEMBRANES<sup><a href="#fn1" rid="fn1" class=" fn">*</a></sup>

W Hubbell

H McConnell

Abstract

The paramagnetic resonance spectra of N-oxyl-4′,′-dimethyloxazolidine derivatives of 12-ketostearic acid, 5-ketostearic acid, 5-ketotricosanoic acid, and 5α-androstan-3-one-17β-ol have been observed in sonicated phospholipid dispersions, the walking-leg nerve fibers of Homarus americanus, and in erythrocytes oriented by hydrodynamic shear. The preferred orientation of these spin labels in the nerve and erythrocyte membranes is one in which the long amphiphilic axis is perpendicular to the membrane surface. The resonance spectra indicate that these labels in the nerve fiber are bound to a lipid bilayer very similar to that found in the sonicated phospholipid dispersion, whereas any lipid bilayer in the erythrocyte must be much more rigid.

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Selected References

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Keith AD, Waggoner AS, Griffith OH. Spin-labeled mitochondrial lipids in Neurospora crassa. Proc Natl Acad Sci U S A. 1968 Nov;61(3):819–826.[PMC free article] [PubMed] [Google Scholar]
Hubbell WL, McConnell HM. Spin-label studies of the excitable membranes of nerve and muscle. Proc Natl Acad Sci U S A. 1968 Sep;61(1):12–16.[PMC free article] [PubMed] [Google Scholar]

STAUFFER LABORATORY FOR PHYSICAL CHEMISTRY, STANFORD, CALIFORNIA

^{Supported by a National Institutes of Health predoctoral fellowship.}

^{Sponsored by the National Institutes of Health under grant NB 08058-01. We are indebted to the Advanced Research Project Agency through the Center for Materials Research at Stanford University for facilities made available for this work.}

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Abstract

The paramagnetic resonance spectra of N-oxyl-4′,′-dimethyloxazolidine derivatives of 12-ketostearic acid, 5-ketostearic acid, 5-ketotricosanoic acid, and 5α-androstan-3-one-17β-ol have been observed in sonicated phospholipid dispersions, the walking-leg nerve fibers of Homarus americanus, and in erythrocytes oriented by hydrodynamic shear. The preferred orientation of these spin labels in the nerve and erythrocyte membranes is one in which the long amphiphilic axis is perpendicular to the membrane surface. The resonance spectra indicate that these labels in the nerve fiber are bound to a lipid bilayer very similar to that found in the sonicated phospholipid dispersion, whereas any lipid bilayer in the erythrocyte must be much more rigid.

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