Ontogenetic Variation of Total Phenolics and Antioxidant Activity in Roots, Leaves and Flowers of Astragalus compactus Lam. (Fabaceae).
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Journal: 2013/May - BioImpacts
ISSN: 2228-5652
Abstract:
BACKGROUND
The potential health risks and toxicity of synthetic antioxidants resulted in an upsurge of interest in phytochemicals as new sources of natural antioxidants. Phenolics of Astragalus L. (Fabaceae) possess antioxidant properties and have been shown to have a protective effect against several degenerative diseases. The objective of this study was to determine total phenolics and antioxidant activity of methanolic extracts from different parts of A. compactus Lam. at different phenological phases and to investigate the correlations between antioxidation and the contents of the total phenolics.
METHODS
Total phenolic content (TPC) was determined using the Folin-Ciocalteau reagent and the antioxidant capacity was evaluated with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) test.
RESULTS
Generally, the TPC in leaves was higher than that of the roots and flowers. TPC in leaves, roots and flowers of the species varied from 5.01-8.25, 4.29-7.89 and 4.19 μg GAE/mg DW, respectively. In addition, roots and leaves at fructification stage possessed higher TPC than vegetative and flowering stages. Therefore, the leaf extracts at fructification phase showed the highest TPC that accompanied with best antioxidant activity. In the root extracts, fructification stage was also characterized by the highest antioxidant activity.
CONCLUSIONS
A positive relationship between antioxidant activity and TPC showed that phenolics were the dominant antioxidant components in the species. The results obtained suggest that A. compactus methanolic extracts may serve as potential sources of natural phenolic antioxidants and that the fructification phase could be considered as the best stage for the harvesting of this plant.
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Bioimpacts 2(2): 105-109
PMID: 23678448

Ontogenetic Variation of Total Phenolics and Antioxidant Activity in Roots, Leaves and Flowers of <em>Astragalus compactus</em> Lam. (Fabaceae)

Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
Drug Applied Research Center, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Horticulture, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Corresponding author: Somayeh Naghiloo (PhD), Tel.: +98-411-3844741, Fax: +98-411-3356027,ri.ca.uzirbat@oolihgan_S
Received 2012 Apr 23; Revised 2012 May 14; Accepted 2012 May 17.
Copyright © 2012 by Tabriz University of Medical Sciences

Abstract

Introduction

The potential health risks and toxicity of synthetic antioxidants resulted in an upsurge of interest in phytochemicals as new sources of natural antioxidants. Phenolics of Astragalus L. (Fabaceae) possess antioxidant properties and have been shown to have a protective effect against several degenerative diseases. The objective of this study was to determine total phenolics and antioxidant activity of methanolic extracts from different parts of A. compactus Lam. at different phenological phases and to investigate the correlations between antioxidation and the contents of the total phenolics.

Methods

Total phenolic content (TPC) was determined using the Folin-Ciocalteau reagent and the antioxidant capacity was evaluated with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) test.

Results

Generally, the TPC in leaves was higher than that of the roots and flowers. TPC in leaves, roots and flowers of the species varied from 5.01-8.25, 4.29-7.89 and 4.19 μg GAE/mg DW, respectively. In addition, roots and leaves at fructification stage possessed higher TPC than vegetative and flowering stages. Therefore, the leaf extracts at fructification phase showed the highest TPC that accompanied with best antioxidant activity. In the root extracts, fructification stage was also characterized by the highest antioxidant activity.

Conclusion

A positive relationship between antioxidant activity and TPC showed that phenolics were the dominant antioxidant components in the species. The results obtained suggest that A. compactus methanolic extracts may serve as potential sources of natural phenolic antioxidants and that the fructification phase could be considered as the best stage for the harvesting of this plant.

Keywords: Astragalus compactus, Antioxidant, Ontogenic Variation, Total Phenolics
Abstract

* Values with different superscript letters are statistically different (p < 0.05).Positive control (quercetin): 0.029 μg/ml.

Ontogenic variation of total phenolic content (TPC) (μg GAE/mg DW) in A. compactus leaf, root, and flower.

Ontogenic variation of antioxidant activity (IC50 (μg/ml)) in A. compactus leaf, root, and flower.

Acknowledgments

This work was funded in part by grants from Research Affairs of the University of Tabriz.

Acknowledgments

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