Nucleic Acids Res 20(7): 1679-1684
Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ.
Abstract
A novel linker for the synthesis of oligonucleotides on a glass support is described. Oligonucleotides synthesised on the support remain tethered to the support after ammonia treatment and are shown to take part in sequence specific hybridisation reactions. These hybridizations were carried out with oligonucleotides synthesised on 'ballotini' solid sphere glass beads and microscope slides. The linker has a hexaethylene glycol spacer, bound to the glass via a glycidoxypropyl silane, terminating in a primary hydroxyl group that serves as starting point for automated or manual oligonucleotide synthesis.
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- Matthews JA, Kricka LJ. Analytical strategies for the use of DNA probes. Anal Biochem. 1988 Feb 15;169(1):1–25. [PubMed] [Google Scholar]
- Wallace RB, Shaffer J, Murphy RF, Bonner J, Hirose T, Itakura K. Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch. Nucleic Acids Res. 1979 Aug 10;6(11):3543–3557.[PMC free article] [PubMed] [Google Scholar]
- Conner BJ, Reyes AA, Morin C, Itakura K, Teplitz RL, Wallace RB. Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides. Proc Natl Acad Sci U S A. 1983 Jan;80(1):278–282.[PMC free article] [PubMed] [Google Scholar]
- Aviv H, Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412.[PMC free article] [PubMed] [Google Scholar]
- Saiki RK, Walsh PS, Levenson CH, Erlich HA. Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6230–6234.[PMC free article] [PubMed] [Google Scholar]
- Zhang Y, Coyne MY, Will SG, Levenson CH, Kawasaki ES. Single-base mutational analysis of cancer and genetic diseases using membrane bound modified oligonucleotides. Nucleic Acids Res. 1991 Jul 25;19(14):3929–3933.[PMC free article] [PubMed] [Google Scholar]
- Kremsky JN, Wooters JL, Dougherty JP, Meyers RE, Collins M, Brown EL. Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus. Nucleic Acids Res. 1987 Apr 10;15(7):2891–2909.[PMC free article] [PubMed] [Google Scholar]
- Van Ness J, Kalbfleisch S, Petrie CR, Reed MW, Tabone JC, Vermeulen NM. A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays. Nucleic Acids Res. 1991 Jun 25;19(12):3345–3350.[PMC free article] [PubMed] [Google Scholar]
- Ghosh SS, Musso GF. Covalent attachment of oligonucleotides to solid supports. Nucleic Acids Res. 1987 Jul 10;15(13):5353–5372.[PMC free article] [PubMed] [Google Scholar]
- Gingeras TR, Kwoh DY, Davis GR. Hybridization properties of immobilized nucleic acids. Nucleic Acids Res. 1987 Jul 10;15(13):5373–5390.[PMC free article] [PubMed] [Google Scholar]
- Khrapko KR, Lysov YuP, Khorlin AA, Ivanov IB, Yershov GM, Vasilenko SK, Florentiev VL, Mirzabekov AD. A method for DNA sequencing by hybridization with oligonucleotide matrix. DNA Seq. 1991;1(6):375–388. [PubMed] [Google Scholar]
- Mandenius CF, Mosbach K, Welin S, Lundström I. Reversible and specific interaction of dehydrogenases with a coenzyme-coated surface continuously monitored with a reflectometer. Anal Biochem. 1986 Sep;157(2):283–288. [PubMed] [Google Scholar]
- Aboul-ela F, Koh D, Tinoco I, Jr, Martin FH. Base-base mismatches. Thermodynamics of double helix formation for dCA3XA3G + dCT3YT3G (X, Y = A,C,G,T). Nucleic Acids Res. 1985 Jul 11;13(13):4811–4824.[PMC free article] [PubMed] [Google Scholar]
- Johnston RF, Pickett SC, Barker DL. Autoradiography using storage phosphor technology. Electrophoresis. 1990 May;11(5):355–360. [PubMed] [Google Scholar]