Three new chromones, namely 10-(2',3'-dihydroxy-3'-methylbutanyl)-spatheliachromen, 10-(1',2',3'-trihydroxy-3'-methylbutanyl)spatheliachromen, and 6-(3'-hydroxy-3'-methyl-trans-but-1'-enyl)-O-methylisospatheliachrome n were isolated from the 10% aqueous MeOH extract of the twigs and leaves of Spathelia sorbifolia, together with five known chromones. Compound 2, the known spatheliabischromen, and 5-O-methylsorbifolin showed cytotoxicity against 9PS cells in culture.