Natamycin as a fungicide in agar media.
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Journal: 2010/June - Applied and Environmental Microbiology
ISSN: 0099-2240
PUBMED: 16348667
Abstract:
Fungal inhibition in four commonly used agar media was improved by substituting natamycin (pimaricin) for cycloheximide. The recovery of bacteria was not affected by natamycin, whereas fungal contamination from a variety of samples was significantly suppressed. Furthermore, natamycin lacks the occupational health hazards of cycloheximide. Medium-dependent natamycin degradation occurred during the preparation and refrigerated storage of agar plates, but the addition of natamycin at 21.6 mug/ml resulted in effective residual activity.
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Appl Environ Microbiol 58(3): 1064-1066
PMID: 16348667

Natamycin as a Fungicide in Agar Media

Abstract

Fungal inhibition in four commonly used agar media was improved by substituting natamycin (pimaricin) for cycloheximide. The recovery of bacteria was not affected by natamycin, whereas fungal contamination from a variety of samples was significantly suppressed. Furthermore, natamycin lacks the occupational health hazards of cycloheximide. Medium-dependent natamycin degradation occurred during the preparation and refrigerated storage of agar plates, but the addition of natamycin at 21.6 μg/ml resulted in effective residual activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Basić-Zaninović T, Papes D, Franekić J. Cycloheximide genotoxicity in in vitro and in vivo test systems. Mutat Res. 1991 Aug;263(4):203–210. [PubMed] [Google Scholar]
  • Kobayashi GS, Medoff G. Antifungal agents: recent developments. Annu Rev Microbiol. 1977;31:291–308. [PubMed] [Google Scholar]
  • VAN UDEN N, DO SOUSA LC. Yeasts from the bovine caecum. J Gen Microbiol. 1957 Apr;16(2):385–395. [PubMed] [Google Scholar]
Department of Marine Ecology and Microbiology, National Environmental Research Institute, Frederiksborgvej 399, P.O. Box 358, DK-4000 Roskilde, Denmark
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Abstract
Fungal inhibition in four commonly used agar media was improved by substituting natamycin (pimaricin) for cycloheximide. The recovery of bacteria was not affected by natamycin, whereas fungal contamination from a variety of samples was significantly suppressed. Furthermore, natamycin lacks the occupational health hazards of cycloheximide. Medium-dependent natamycin degradation occurred during the preparation and refrigerated storage of agar plates, but the addition of natamycin at 21.6 μg/ml resulted in effective residual activity.
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