Mitogenic activity from trout embryos.
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Journal: 1990/June - Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
PUBMED: 2333296
Abstract:
An extract of 21-day rainbow trout embryos stimulated growth of several piscine cell lines in the absence of added serum. Established lines from trout (RTG-2 and STE-137), salmon (CHSE-214), carp (EPC), and goldfish (CAR) and early-passage cells initiated from trout embryos grew in serum-free medium containing the embryo extract. In addition the extract was sufficient for maintaining long-term cultures of CHSE-214 cells for several months through a minimum of 20 passages (approximately 50 population doublings) in the absence of serum. Optimal response was achieved with 100 micrograms of extract protein per ml, but a significant growth-promoting effect was observed with as little as 2.5 micrograms/ml. The activity was nondialyzable, protease-sensitive, and stable in 200 mM acetic acid. The level of mitogenic response induced by the extract could not be duplicated with purified mammalian growth factors added individually or in combination, and the extract did not stimulate DNA synthesis in quiescent mouse fibroblasts. These results suggest that trout embryo extract may contain a novel growth-promoting activity for fish cells.
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Proc Natl Acad Sci U S A 87(9): 3498-3502
PMID: 2333296

Mitogenic activity from trout embryos.

Abstract

An extract of 21-day rainbow trout embryos stimulated growth of several piscine cell lines in the absence of added serum. Established lines from trout (RTG-2 and STE-137), salmon (CHSE-214), carp (EPC), and goldfish (CAR) and early-passage cells initiated from trout embryos grew in serum-free medium containing the embryo extract. In addition the extract was sufficient for maintaining long-term cultures of CHSE-214 cells for several months through a minimum of 20 passages (approximately 50 population doublings) in the absence of serum. Optimal response was achieved with 100 micrograms of extract protein per ml, but a significant growth-promoting effect was observed with as little as 2.5 micrograms/ml. The activity was nondialyzable, protease-sensitive, and stable in 200 mM acetic acid. The level of mitogenic response induced by the extract could not be duplicated with purified mammalian growth factors added individually or in combination, and the extract did not stimulate DNA synthesis in quiescent mouse fibroblasts. These results suggest that trout embryo extract may contain a novel growth-promoting activity for fish cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Streisinger G, Coale F, Taggart C, Walker C, Grunwald DJ. Clonal origins of cells in the pigmented retina of the zebrafish eye. Dev Biol. 1989 Jan;131(1):60–69. [PubMed] [Google Scholar]
  • Streisinger G, Walker C, Dower N, Knauber D, Singer F. Production of clones of homozygous diploid zebra fish (Brachydanio rerio). Nature. 1981 May 28;291(5813):293–296. [PubMed] [Google Scholar]
  • Kuwada JY. Cell recognition by neuronal growth cones in a simple vertebrate embryo. Science. 1986 Aug 15;233(4765):740–746. [PubMed] [Google Scholar]
  • Streisinger G. Attainment of minimal biological variability and measurements of genotoxicity: production of homozygous diploid zebra fish. Natl Cancer Inst Monogr. 1984 May;65:53–58. [PubMed] [Google Scholar]
  • Anders F, Schartl M, Barnekow A. Xiphophorus as an in vivo model for studies on oncogenes. Natl Cancer Inst Monogr. 1984 May;65:97–109. [PubMed] [Google Scholar]
  • Hendricks JD, Meyers TR, Casteel JL, Nixon JE, Loveland PM, Bailey GS. Rainbow trout embryos: advantages and limitations for carcinogenesis research. Natl Cancer Inst Monogr. 1984 May;65:129–137. [PubMed] [Google Scholar]
  • Parsons JE, Thorgaard GH. Production of androgenetic diploid rainbow trout. J Hered. 1985 May-Jun;76(3):177–181. [PubMed] [Google Scholar]
  • Wales JH, Sinnhuber RO, Hendricks JD, Nixon JE, Eisele TA. Aflatoxin B1 induction of hepatocellular carcinoma in the embryos of rainbow trout (Salmo gairdneri). J Natl Cancer Inst. 1978 May;60(5):1133–1139. [PubMed] [Google Scholar]
  • Hendricks JD, Scanlan RA, Williams JL, Sinnhuber RO, Grieco MP. Carcinogenicity of N-methyl-N'-nitro-N-nitrosoguanidine to the livers and kidneys of rainbow trout (Salmo gairdneri) exposed as embryos. J Natl Cancer Inst. 1980 Jun;64(6):1511–1519. [PubMed] [Google Scholar]
  • Powers DA. Fish as model systems. Science. 1989 Oct 20;246(4928):352–358. [PubMed] [Google Scholar]
  • Hightower LE, Renfro JL. Recent applications of fish cell culture to biomedical research. J Exp Zool. 1988 Dec;248(3):290–302. [PubMed] [Google Scholar]
  • Fryer JL, Yusha A, Pilcher KS. The in vitro cultivation of tissue and cells of Pacific salmon and steelhead trout. Ann N Y Acad Sci. 1965 Aug 10;126(1):566–586. [PubMed] [Google Scholar]
  • Lee LE, Martinez A, Bols NC. Culture conditions for arresting and stimulating the proliferation of a rainbow trout fibroblast cell line, RTG-2. In Vitro Cell Dev Biol. 1988 Aug;24(8):795–802. [PubMed] [Google Scholar]
  • Barnes D, Sato G. Serum-free cell culture: a unifying approach. Cell. 1980 Dec;22(3):649–655. [PubMed] [Google Scholar]
  • Wolf K, Mann JA. Poikilotherm vertebrate cell lines and viruses: a current listing for fishes. In Vitro. 1980 Feb;16(2):168–179. [PubMed] [Google Scholar]
  • Barnes DW, Colowick SP. Stimulation of sugar uptake and thymidine incorporation in mouse 3T3 cells by calcium phosphate and other extracellular particles. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5593–5597.[PMC free article] [PubMed] [Google Scholar]
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed] [Google Scholar]
  • Loo DT, Fuquay JI, Rawson CL, Barnes DW. Extended culture of mouse embryo cells without senescence: inhibition by serum. Science. 1987 Apr 10;236(4798):200–202. [PubMed] [Google Scholar]
  • Chiang LC, Silnutzer J, Pipas JM, Barnes DW. Selection of transformed cells in serum-free media. In Vitro Cell Dev Biol. 1985 Dec;21(12):707–712. [PubMed] [Google Scholar]
  • Rosa F, Roberts AB, Danielpour D, Dart LL, Sporn MB, Dawid IB. Mesoderm induction in amphibians: the role of TGF-beta 2-like factors. Science. 1988 Feb 12;239(4841 Pt 1):783–785. [PubMed] [Google Scholar]
  • Kimelman D, Kirschner M. Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo. Cell. 1987 Dec 4;51(5):869–877. [PubMed] [Google Scholar]
  • Slack JM, Darlington BG, Heath JK, Godsave SF. Mesoderm induction in early Xenopus embryos by heparin-binding growth factors. Nature. 1987 Mar 12;326(6109):197–200. [PubMed] [Google Scholar]
  • de Pablo F, Chambers SA, Ota A. Insulin-related molecules and insulin effects in the sea urchin embryo. Dev Biol. 1988 Nov;130(1):304–310. [PubMed] [Google Scholar]
  • Singh JP, Chaikin MA, Stiles CD. Phylogenetic analysis of platelet-derived growth factor by radio-receptor assay. J Cell Biol. 1982 Nov;95(2 Pt 1):667–671.[PMC free article] [PubMed] [Google Scholar]
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-6503.
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-6503.
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Abstract
An extract of 21-day rainbow trout embryos stimulated growth of several piscine cell lines in the absence of added serum. Established lines from trout (RTG-2 and STE-137), salmon (CHSE-214), carp (EPC), and goldfish (CAR) and early-passage cells initiated from trout embryos grew in serum-free medium containing the embryo extract. In addition the extract was sufficient for maintaining long-term cultures of CHSE-214 cells for several months through a minimum of 20 passages (approximately 50 population doublings) in the absence of serum. Optimal response was achieved with 100 micrograms of extract protein per ml, but a significant growth-promoting effect was observed with as little as 2.5 micrograms/ml. The activity was nondialyzable, protease-sensitive, and stable in 200 mM acetic acid. The level of mitogenic response induced by the extract could not be duplicated with purified mammalian growth factors added individually or in combination, and the extract did not stimulate DNA synthesis in quiescent mouse fibroblasts. These results suggest that trout embryo extract may contain a novel growth-promoting activity for fish cells.
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