Human transferrin: cDNA characterization and chromosomal localization.
PDF (1.0M)
+3 authors
Journal: 1984/June - Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
PUBMED: 6585826
Abstract:
Transferrin (Tf) is the major iron binding protein in vertebrate serum. It shares homologous amino acid sequences with four other proteins: lactotransferrin, ovotransferrin, melanoma antigen p97, and HuBlym-1. Antigen p97 and the Tf receptor genes have been mapped on human chromosome 3. The goal of the study described here was to initiate the characterization of the Tf gene by identifying and characterizing its cDNA and mapping its chromosomal location. Recombinant plasmids containing human cDNA encoding Tf have been isolated by screening an adult human liver library with a mixed oligonucleotide probe. Within the 2.3 kilobase pairs of Tf cDNA analyzed, there is a probable leader sequence encoded by 57 nucleotides followed by 2037 nucleotides that encode the homologous amino and carboxyl domains. During evolution, three areas of the homologous amino and carboxyl domains have been strongly conserved, possibly reflecting functional constraints associated with iron binding. Chromosomal mapping by in situ hybridization and somatic cell hybrid analysis indicate that the Tf gene is located at q21-25 on human chromosome 3, consistent with linkage of the Tf, Tf receptor, and melanoma p97 loci.
Open in
PUBMED | PMC | Google Scholar | Wikipedia
Relations:
Content
Citations
(63)
References
(30)
Chemicals
(1)
Genes
(1)
Organisms
(1)
Processes
(2)
Anatomy
(1)
Similar articles
Articles by the same authors
Discussion board
Proc Natl Acad Sci U S A 81(9): 2752-2756
PMID: 6585826

Human transferrin: cDNA characterization and chromosomal localization.

Abstract

Transferrin (Tf) is the major iron binding protein in vertebrate serum. It shares homologous amino acid sequences with four other proteins: lactotransferrin, ovotransferrin, melanoma antigen p97, and HuBlym-1. Antigen p97 and the Tf receptor genes have been mapped on human chromosome 3. The goal of the study described here was to initiate the characterization of the Tf gene by identifying and characterizing its cDNA and mapping its chromosomal location. Recombinant plasmids containing human cDNA encoding Tf have been isolated by screening an adult human liver library with a mixed oligonucleotide probe. Within the 2.3 kilobase pairs of Tf cDNA analyzed, there is a probable leader sequence encoded by 57 nucleotides followed by 2037 nucleotides that encode the homologous amino and carboxyl domains. During evolution, three areas of the homologous amino and carboxyl domains have been strongly conserved, possibly reflecting functional constraints associated with iron binding. Chromosomal mapping by in situ hybridization and somatic cell hybrid analysis indicate that the Tf gene is located at q21-25 on human chromosome 3, consistent with linkage of the Tf, Tf receptor, and melanoma p97 loci.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
icon of scanned page 2752
2752
icon of scanned page 2753
2753
icon of scanned page 2754
2754
icon of scanned page 2755
2755
icon of scanned page 2756
2756

Images in this article

Image
Image
on p.2755
Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • MacGillivray RT, Mendez E, Shewale JG, Sinha SK, Lineback-Zins J, Brew K. The primary structure of human serum transferrin. The structures of seven cyanogen bromide fragments and the assembly of the complete structure. J Biol Chem. 1983 Mar 25;258(6):3543–3553. [PubMed] [Google Scholar]
  • Jeltsch JM, Chambon P. The complete nucleotide sequence of the chicken ovotransferrin mRNA. Eur J Biochem. 1982 Feb;122(2):291–295. [PubMed] [Google Scholar]
  • Williams J, Elleman TC, Kingston IB, Wilkins AG, Kuhn KA. The primary structure of hen ovotransferrin. Eur J Biochem. 1982 Feb;122(2):297–303. [PubMed] [Google Scholar]
  • Metz-Boutigue MH, Mazurier J, Jollès J, Spik G, Montreuil J, Jollès P. The present state of the human lactotransferrin sequence. Study and alignment of the cyanogen bromide fragments and characterization of N- and C-terminal domains. Biochim Biophys Acta. 1981 Sep 29;670(2):243–254. [PubMed] [Google Scholar]
  • Brown JP, Hewick RM, Hellström I, Hellström KE, Doolittle RF, Dreyer WJ. Human melanoma-associated antigen p97 is structurally and functionally related to transferrin. Nature. 1982 Mar 11;296(5853):171–173. [PubMed] [Google Scholar]
  • Goubin G, Goldman DS, Luce J, Neiman PE, Cooper GM. Molecular cloning and nucleotide sequence of a transforming gene detected by transfection of chicken B-cell lymphoma DNA. Nature. 1983 Mar 10;302(5904):114–119. [PubMed] [Google Scholar]
  • Diamond A, Cooper GM, Ritz J, Lane MA. Identification and molecular cloning of the human Blym transforming gene activated in Burkitt's lymphomas. Nature. 1983 Sep 8;305(5930):112–116. [PubMed] [Google Scholar]
  • Alvarado-Urbina G, Sathe GM, Liu WC, Gillen MF, Duck PD, Bender R, Ogilvie KK. Automated synthesis of gene fragments. Science. 1981 Oct 16;214(4518):270–274. [PubMed] [Google Scholar]
  • Prochownik EV, Markham AF, Orkin SH. Isolation of a cDNA clone for human antithrombin III. J Biol Chem. 1983 Jul 10;258(13):8389–8394. [PubMed] [Google Scholar]
  • Grunstein M, Hogness DS. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965.[PMC free article] [PubMed] [Google Scholar]
  • Wallace RB, Johnson MJ, Hirose T, Miyake T, Kawashima EH, Itakura K. The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA. Nucleic Acids Res. 1981 Feb 25;9(4):879–894.[PMC free article] [PubMed] [Google Scholar]
  • Denhardt DT. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. [PubMed] [Google Scholar]
  • Clewell DB, Helinski DR. Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli. J Bacteriol. 1972 Jun;110(3):1135–1146.[PMC free article] [PubMed] [Google Scholar]
  • Blair DG, Sherratt DJ, Clewell DB, Helinski DR. Isolation of supercoiled colicinogenic factor E 1 DNA sensitive to ribonuclease and alkali. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2518–2522.[PMC free article] [PubMed] [Google Scholar]
  • Tu CP, Cohen SN. 3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate. Gene. 1980 Jul;10(2):177–183. [PubMed] [Google Scholar]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed] [Google Scholar]
  • Sargent TD, Yang M, Bonner J. Nucleotide sequence of cloned rat serum albumin messenger RNA. Proc Natl Acad Sci U S A. 1981 Jan;78(1):243–246.[PMC free article] [PubMed] [Google Scholar]
  • Harper ME, Saunders GF. Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma. 1981;83(3):431–439. [PubMed] [Google Scholar]
  • Naylor SL, Elliott RW, Brown JA, Shows TB. Mapping of aminoacylase-1 and beta-galactosidase-A to homologous regions of human chromosome 3 and mouse chromosome 9 suggests location of additional genes. Am J Hum Genet. 1982 Mar;34(2):235–244.[PMC free article] [PubMed] [Google Scholar]
  • Thibodeau SN, Lee DC, Palmiter RD. Identical precursors for serum transferrin and egg white conalbumin. J Biol Chem. 1978 Jun 10;253(11):3771–3774. [PubMed] [Google Scholar]
  • Wang AC, Sutton HE. Human Transferrins C and D1: Chemical Difference in a Peptide. Science. 1965 Jul 23;149(3682):435–437. [PubMed] [Google Scholar]
  • Wang AC, Sutton HE, Howard PN. Human transferrins C and D-Chi: an amino acid difference. Biochem Genet. 1967 Jun;1(1):55–59. [PubMed] [Google Scholar]
  • Wang AC, Sutton HE, Riggs A. A chemical difference between human transferrins B2 and C. Am J Hum Genet. 1966 Sep;18(5):454–458.[PMC free article] [PubMed] [Google Scholar]
  • Yang F, Brune JL, Baldwin WD, Barnett DR, Bowman BH. Identification and characterization of human haptoglobin cDNA. Proc Natl Acad Sci U S A. 1983 Oct;80(19):5875–5879.[PMC free article] [PubMed] [Google Scholar]
  • SMITHIES O, CONNELL GE, DIXON GH. Chromosomal rearrangements and the evolution of haptoglobin genes. Nature. 1962 Oct 20;196:232–236. [PubMed] [Google Scholar]
  • Naylor SL, Elliott RW, Brown JA, Shows TB. Mapping of aminoacylase-1 and beta-galactosidase-A to homologous regions of human chromosome 3 and mouse chromosome 9 suggests location of additional genes. Am J Hum Genet. 1982 Mar;34(2):235–244.[PMC free article] [PubMed] [Google Scholar]
  • Plowman GD, Brown JP, Enns CA, Schröder J, Nikinmaa B, Sussman HH, Hellström KE, Hellström I. Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3. Nature. 1983 May 5;303(5912):70–72. [PubMed] [Google Scholar]
  • Goodfellow PN, Banting G, Sutherland R, Greaves M, Solomon E, Povey S. Expression of human transferrin receptor is controlled by a gene on chromosome 3: assignment using species specificity of a monoclonal antibody. Somatic Cell Genet. 1982 Mar;8(2):197–206. [PubMed] [Google Scholar]
  • Enns CA, Suomalainen HA, Gebhardt JE, Schröder J, Sussman HH. Human transferrin receptor: expression of the receptor is assigned to chromosome 3. Proc Natl Acad Sci U S A. 1982 May;79(10):3241–3245.[PMC free article] [PubMed] [Google Scholar]
  • Miller YE, Jones C, Scoggin C, Morse H, Seligman P. Chromosome 3q (22-ter) encodes the human transferrin receptor. Am J Hum Genet. 1983 Jul;35(4):573–583.[PMC free article] [PubMed] [Google Scholar]
  • McKusick VA, Conneally PM. Report of the Committee on Unassigned Linkage Groups. Cytogenet Cell Genet. 1984;37(1-4):205–209. [PubMed] [Google Scholar]
Copyright notice
Abstract
Transferrin (Tf) is the major iron binding protein in vertebrate serum. It shares homologous amino acid sequences with four other proteins: lactotransferrin, ovotransferrin, melanoma antigen p97, and HuBlym-1. Antigen p97 and the Tf receptor genes have been mapped on human chromosome 3. The goal of the study described here was to initiate the characterization of the Tf gene by identifying and characterizing its cDNA and mapping its chromosomal location. Recombinant plasmids containing human cDNA encoding Tf have been isolated by screening an adult human liver library with a mixed oligonucleotide probe. Within the 2.3 kilobase pairs of Tf cDNA analyzed, there is a probable leader sequence encoded by 57 nucleotides followed by 2037 nucleotides that encode the homologous amino and carboxyl domains. During evolution, three areas of the homologous amino and carboxyl domains have been strongly conserved, possibly reflecting functional constraints associated with iron binding. Chromosomal mapping by in situ hybridization and somatic cell hybrid analysis indicate that the Tf gene is located at q21-25 on human chromosome 3, consistent with linkage of the Tf, Tf receptor, and melanoma p97 loci.
Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.