Host cell reactivation of Bacillus subtilis bacteriophages.
PDF (962K)
Journal: 1977/September - Journal of Bacteriology
ISSN: 0021-9193
PUBMED: 407209
Abstract:
Host cell reactivation of ultraviolet-irradiated phage can be used as a probe of the bacterial repair system and to determine phage and cellular contributions to the repair process. Using the Bacillus subtilis phages SPP1, SP01, phie, and phi29, we found that the uvr-1 and polA functions are involved in the host cell reactivation of the four phages. SPP1 was the only phage whose reactivation was also decreased in recA, recD, and recF mutant cells. We studied variations of host cell reactivation for SPP1 during spore outgrowth; at high ultraviolet doses the activity of a spore repair system requiring deoxyribonucleic acid polymerase I became evident. The spore repair system was completely replaced by the vegetative one by 120 min of outgrowth.
Open in
PUBMED | PMC | Google Scholar | Wikipedia
Relations:
Content
Citations
(2)
References
(37)
Chemicals
(2)
Organisms
(2)
Processes
(4)
Anatomy
(1)
Similar articles
Articles by the same authors
Discussion board
J Bacteriol 131(2): 382-388
PMID: 407209

Host cell reactivation of Bacillus subtilis bacteriophages.

Abstract

Host cell reactivation of ultraviolet-irradiated phage can be used as a probe of the bacterial repair system and to determine phage and cellular contributions to the repair process. Using the Bacillus subtilis phages SPP1, SP01, phie, and phi29, we found that the uvr-1 and polA functions are involved in the host cell reactivation of the four phages. SPP1 was the only phage whose reactivation was also decreased in recA, recD, and recF mutant cells. We studied variations of host cell reactivation for SPP1 during spore outgrowth; at high ultraviolet doses the activity of a spore repair system requiring deoxyribonucleic acid polymerase I became evident. The spore repair system was completely replaced by the vegetative one by 120 min of outgrowth.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (962K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
icon of scanned page 382
382
icon of scanned page 383
383
icon of scanned page 384
384
icon of scanned page 385
385
icon of scanned page 386
386
icon of scanned page 387
387
icon of scanned page 388
388

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Anderson DL, Hickman DD, Reilly BE. Structure of Bacillus subtilis bacteriophage phi 29 and the length of phi 29 deoxyribonucleic acid. J Bacteriol. 1966 May;91(5):2081–2089.[PMC free article] [PubMed] [Google Scholar]
  • Biswal N, Kleinschmidt AK, Spatz HC, Trautner TA. Physical properties of the DNA of bacteriophage SP50. Mol Gen Genet. 1967;100(1):39–55. [PubMed] [Google Scholar]
  • BOYCE RP, HOWARD-FLANDERS P. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12. Proc Natl Acad Sci U S A. 1964 Feb;51:293–300.[PMC free article] [PubMed] [Google Scholar]
  • Donnellan JE, Jr, Setlow RB. Thymine Photoproducts but not Thymine Dimers Found in Ultraviolet-Irradiated Bacterial Spores. Science. 1965 Jul 16;149(3681):308–310. [PubMed] [Google Scholar]
  • Donnellan JE, Jr, Stafford RS. The ultraviolet photochemistry and photobiology of vegetative cells and spores of Bacillus megaterium. Biophys J. 1968 Jan;8(1):17–28.[PMC free article] [PubMed] [Google Scholar]
  • Doudney CO. Ultraviolet light effects on the bacterial cell. Curr Top Microbiol Immunol. 1968;46:116–175. [PubMed] [Google Scholar]
  • Elder RL, Beers RF. Nonphotoreactivating Repair of Ultraviolet Light-Damaged Transforming Deoxyribonucleic Acid by Micrococcus lysodeikticus Extracts. J Bacteriol. 1965 Sep;90(3):681–686.[PMC free article] [PubMed] [Google Scholar]
  • ELLISON SA, FEINER RR, HILL RF. A host effect on bacteriophage survival after ultraviolet irradiation. Virology. 1960 May;11:294–296. [PubMed] [Google Scholar]
  • Falaschi A, Kornberg A. Biochemical studies of bacterial sporulation. II. Deoxy- ribonucleic acid polymerase in spores of Bacillus subtilis. J Biol Chem. 1966 Apr 10;241(7):1478–1482. [PubMed] [Google Scholar]
  • Ganesan AT, Yehle CO, Yu CC. DNA replication in a polymerase I deficient mutant and the identification of DNA polymerases II and 3 in Bacillus subtilis. Biochem Biophys Res Commun. 1973 Jan 4;50(1):155–163. [PubMed] [Google Scholar]
  • Gass KB, Hill TC, Goulian M, Strauss BS, Cozzarelli NR. Altered deoxyribonucleic acid polymerase activity in a methyl methanesulfonate-sensitive mutant of Bacillus subtilis. J Bacteriol. 1971 Oct;108(1):364–374.[PMC free article] [PubMed] [Google Scholar]
  • HARM W. On the relationship between host-cell reactivation and UV-reactivation in UV-inactivated phages. Z Vererbungsl. 1963;94:67–79. [PubMed] [Google Scholar]
  • Hoch JA, Anagnostopoulos C. Chromosomal location and properties of radiation sensitivity mutations in Bacillus subtilis. J Bacteriol. 1970 Aug;103(2):295–301.[PMC free article] [PubMed] [Google Scholar]
  • HOWARD-FLANDERS P, BOYCE RP, SIMSON E, THERIOT L. A genetic locus in E. coli K12 that controls the reactivation of UV-photoproducts associated with thymine in DNA. Proc Natl Acad Sci U S A. 1962 Dec 15;48:2109–2115.[PMC free article] [PubMed] [Google Scholar]
  • Laipis PJ, Ganesan AT. A deoxyribonucleic acid polymerase I-deficient mutant of Bacillus subtilis. J Biol Chem. 1972 Sep 25;247(18):5867–5871. [PubMed] [Google Scholar]
  • Mazza G, Fortunato A, Ferrari E, Canosi U, Falaschi A, Polsinelli M. Genetic and enzymic studies on the recombination process in Bacillus subtilis. Mol Gen Genet. 1975;136(1):9–30. [PubMed] [Google Scholar]
  • Munakata N, Rupert CS. Dark repair of DNA containing "spore photoproduct" in Bacillus subtilis. Mol Gen Genet. 1974 May 31;130(3):239–250. [PubMed] [Google Scholar]
  • Munakata N, Rupert CS. Effects of DNA-polymerase-defective and recombination-deficient mutations on the ultraviolet sensitivity of Bacillus subtilis spores. Mutat Res. 1975 Feb;27(2):157–169. [PubMed] [Google Scholar]
  • Okubo S, Romig WR. Comparison of ultraviolet sensitivity of Bacillus subtilis bacteriophage SPO2 and its infectious DNA. J Mol Biol. 1965 Nov;14(1):130–142. [PubMed] [Google Scholar]
  • Okubo S, Romig WR. Impaired transformability of Bacillus subtilis mutant sensitive to mitomycin C and ultraviolet radiation. J Mol Biol. 1966 Feb;15(2):440–454. [PubMed] [Google Scholar]
  • OKUBO S, STRAUSS B, STODOLSKY M. THE POSSIBLE ROLE OF RECOMBINATION IN THE INFECTION OF COMPETENT BACILLUS SUBTILIS BY BACTERIOPHAGE DEOXYRIBONUCLEIC ACID. Virology. 1964 Dec;24:552–562. [PubMed] [Google Scholar]
  • PETTIJOHN D, HANAWALT P. EVIDENCE FOR REPAIR-REPLICATION OF ULTRAVIOLET DAMAGED DNA IN BACTERIA. J Mol Biol. 1964 Aug;9:395–410. [PubMed] [Google Scholar]
  • Reiter H, Strauss B. Repair of damage induced by a monofunctional alkylating agent in a transformable, ultraviolet-sensitive strain of Bacillus subtilis. J Mol Biol. 1965 Nov;14(1):179–194. [PubMed] [Google Scholar]
  • Riklis E. Studies on mechanism of repair of ultraviolet-irradiated viral and bacterial DNAin vivo and in vitro. Can J Biochem. 1965 Jul;43(7):1207–1219. [PubMed] [Google Scholar]
  • Riva S, Polsinelli M, Falaschi A. A new phage of Bacillus subtilis with infectious DNA having separable strands. J Mol Biol. 1968 Jul 28;35(2):347–356. [PubMed] [Google Scholar]
  • RORSCH A, EDELMAN A, COHEN JA. The gene-controlled radiation sensitivity in Escherichia coli. Biochim Biophys Acta. 1963 Feb 26;68:263–270. [PubMed] [Google Scholar]
  • ROERSCH A, VAN DER KAM PC, ADEMA J. DARK REACTIVATION OF ULTRAVIOLET IRRADIATED BACTERIOPHAGE DEOXYRIBONUCLEIC ACID IN VITRO. Biochim Biophys Acta. 1964 Feb 17;80:346–348. [PubMed] [Google Scholar]
  • SAUERBIER W. Evidence for a nonrecombinational mechanism of host cell reactivation of phage. Virology. 1962 Apr;16:398–404. [PubMed] [Google Scholar]
  • SETLOW RB, CARRIER WL. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM. Proc Natl Acad Sci U S A. 1964 Feb;51:226–231.[PMC free article] [PubMed] [Google Scholar]
  • Terano H, Tanooka H, Kadota H. Germination-induced repair of single-strand breaks of DNA in irradiated Bacillus subtilis spores. Biochem Biophys Res Commun. 1969 Sep 24;37(1):66–71. [PubMed] [Google Scholar]
  • Terano H, Tanooka H, Kadota H. Repair of radiation damage to deoxyribonucleic acid in germinating spores of Bacillus subtilis. J Bacteriol. 1971 Jun;106(3):925–930.[PMC free article] [PubMed] [Google Scholar]
  • Tyrrell RM, Moss SH, Davies DJ. The variation in UV sensitivity of four K12 strains of Escherichia coli as a function of their stage of growth. Mutat Res. 1972 Sep;16(7):1–12. [PubMed] [Google Scholar]
  • Wang TC, Rupert CS. Transitory germinative excision repair in Bacillus subtilis. J Bacteriol. 1977 Mar;129(3):1313–1319.[PMC free article] [PubMed] [Google Scholar]
  • Villani G, Canosi U, Fortunato A, Mazza G, Polsinelli M, Falaschi A. Properties of a Bacillus subtilis strain lacking DNA polymerase I. Nucleic Acids Res. 1974 Mar;1(3):461–477.[PMC free article] [PubMed] [Google Scholar]
  • Zamenhof S, Reddy TK. Induction of mutations by ultraviolet irradiation of spores of Bacillus subtilis. Radiat Res. 1967 May;31(1):112–120. [PubMed] [Google Scholar]
  • Yehle CO, Ganesan AT. Deoxyribonucleic acid synthesis in bacteriophage SPO1-infected Bacillus subtilis. I. Bacteriophage deoxyribonucleic acid synthesis and fate of host deoxyribonucleic acid in normal and polymerase-deficient strains. J Virol. 1972 Feb;9(2):263–272.[PMC free article] [PubMed] [Google Scholar]
  • Yehle CO, Ganesan AT. Deoxyribonucleic acid synthesis in bacteriophage SP01-infected Bacillus subtilis. II. Purification and catalytic properties of a deoxyribonucleic acid polymerase induced after infection. J Biol Chem. 1973 Nov 10;248(21):7456–7463. [PubMed] [Google Scholar]
Copyright notice
Abstract
Host cell reactivation of ultraviolet-irradiated phage can be used as a probe of the bacterial repair system and to determine phage and cellular contributions to the repair process. Using the Bacillus subtilis phages SPP1, SP01, phie, and phi29, we found that the uvr-1 and polA functions are involved in the host cell reactivation of the four phages. SPP1 was the only phage whose reactivation was also decreased in recA, recD, and recF mutant cells. We studied variations of host cell reactivation for SPP1 during spore outgrowth; at high ultraviolet doses the activity of a spore repair system requiring deoxyribonucleic acid polymerase I became evident. The spore repair system was completely replaced by the vegetative one by 120 min of outgrowth.
Collaboration tool especially designed for Life Science professionals.Drag-and-drop any entity to your messages.